Enteroviruses and Parechoviruses

Jd, Cherry

doi:10.1128/9781555816728.ch85

Cited by 9 publications

(1 citation statement)

References 103 publications

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“…The sensitivity of cell cultures for rhino/enterovirus was also low and could be partially attributed to the fact that group C rhinoviruses do not grow on standard cell cultures (Jacobs et al, 2013). Enteroviruses grow inconsistently on cell cultures, depending on their type, and no cell line enables the detection of all strains (Stellrecht et al, 2011). Unfortunately, rhinoviruses and enteroviruses in this study were not typed.…”

Section: Analytical Performances and Influencing Factors Of Non-molecmentioning

confidence: 83%

Prospective evaluation of diagnostic tools for respiratory viruses in children and adults

Busson¹,

Bartiaux

Brahim

et al. 2019

Journal of Virological Methods

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A B S T R A C TAim: To compare the performances of molecular and non-molecular tests to diagnose respiratory viral infections and to evaluate the pros and contras of each technique. Methods: Two hundred ninety-nine respiratory samples were prospectively explored using multiplex molecular techniques (FilmArray Respiratory Panel, Clart Pneumovir), immunological techniques (direct fluorescent assay, lateral flow chromatography) and cell cultures. Findings: Molecular techniques permitted the recovery of up to 50% more respiratory pathogens in comparison to non-molecular methods. FilmArray detected at least 30% more pathogens than Clart Pneumovir which could be explained by the differences in their technical designs. The turnaround time under 2 hours for the FilmArray permitted delivery of results when patients were still in the emergency room.⁎ Corresponding author at: LHUB-ULB -Microbiology,

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Section: Analytical Performances and Influencing Factors Of Non-molecmentioning

confidence: 83%

Prospective evaluation of diagnostic tools for respiratory viruses in children and adults

Busson¹,

Bartiaux

Brahim

et al. 2019

Journal of Virological Methods

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Enteroviruses in Humans

Romero

2012

Encyclopedia of Life Sciences

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Enteroviruses are significant ribonucleic acid (RNA) viral pathogens of humans with a worldwide distribution. Humans are the only known hosts for the enteroviruses. Their major route of infection is that of the faecal–oral route. In temperate climates enteroviral infection occurs primarily in the summer and early fall. Infants represent the majority of cases. Enteroviral infection results in viremia and prolonged gastrointestinal shedding of the virus. Enteroviral syndromes involve almost every organ system. Although the majority of infections are asymptomatic or result in a self‐limited infection, fatalities do occur. Especially in neonates or individuals with B‐cell immunodeficiencies. Advances in technology are providing improved understanding of their taxonomy, epidemiology and means of detection. Key Concepts: Enteroviruses have RNA genomes. The 5′NTR of the enteroviruses contain areas of conserved nucleotide identity. Originally five species were described within the genus: polioviruses, the group A and B coxsackievirus, the echoviruses and the numbered enteroviruses. Using modern molecular virologic methods the species have been redefined and novel members of the genus have been identified. Enteroviruses have a worldwide distribution and exhibit strong seasonality. The coding sequence of VP1 is used for the detailed analysis of enteroviruses. Humans are the sole reservoir for the enteroviruses. Infections by enteroviruses are significantly higher in infants. Faecal–oral transmission is the major mode of transmission of the enteroviruses. Infection results in prolonged shedding of the enteroviruses. Understanding the pathophysiology of the enteroviruses is based on the study of prototypic members of the genus: the polioviruses. The major determinant of susceptibility to the enteroviruses is the presence of the receptor required for viral attachment. Reverse transcription‐polymerase chain reaction (RT‐PCR) has replaced tissue culture and mouse inoculation for detection of the enteroviruses. RT‐PCR is more sensitive and faster for the detection of the enteroviruses than tissue culture or mouse inoculation. RT‐PCR diagnosis of enteroviral infections can significantly impact on patient care. Management of enteroviral infections is supportive in nature.

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Pediatric Group B Coxsackievirus Infections

Romero

Current Topics in Microbiology and Immunology

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Enteroviruses and Parechoviruses

Cited by 9 publications

References 103 publications

Prospective evaluation of diagnostic tools for respiratory viruses in children and adults

Prospective evaluation of diagnostic tools for respiratory viruses in children and adults

Enteroviruses in Humans

Pediatric Group B Coxsackievirus Infections

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