Enterotoxigenic Escherichia coli and Staphylococcus aureus in fish and seafood from the southern region of Brazil

Ayulo, Andres Mane Romero; Machado, Ruben Abreu; Scussel, V. M.

doi:10.1016/0168-1605(94)90116-3

Cited by 80 publications

(59 citation statements)

References 14 publications

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“…However, in a study conducted in the coastal region of Santa Catarina, Ayulo et al (2) observed a 20% contamination rate with Staphylococcus aureus of fish, crab, shrimp and bivalve meat samples. These authors detected that 60% of the bivalve meat samples contained coagulase positive S. aureus.…”

Section: Resultsmentioning

confidence: 99%

“…coli (Table 4) was detected in 9% (4/45) of oyster samples collected in the cultivation area, and in 35.5% (16/45) of samples obtained from commercial establishments. Eyles and Davey (6) detected E. coli in 43% of all samples, and Ayulo et al (2) reported contamination with E. coli in 37.7% of fish, crab, shrimp and bivalve meat samples. This high prevalence of E. coli agrees with the present results.…”

Section: Resultsmentioning

confidence: 99%

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Microbiological quality of oysters (Crassostrea gigas) produced and commercialized in the coastal region of Florianópolis - Brazil

Pereira¹,

Nunes²,

Nuernberg³

et al. 2006

Braz. J. Microbiol.

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Oysters are filter feeders able to ingest particles in suspension that may carry pathogenic microorganisms. In this respect, the consumption of raw oysters can cause foodborne diseases in humans. The aim of the present study was to evaluate the microbiological quality of Crassostrea gigas oysters cultivated and commercialized in the coastal region of Florianópolis, SC, Brazil. The study comprised counts of coliforms at 35ºC and at 45ºC, Escherichia coli and coagulase-positive staphylococci, and detection of Salmonella sp, Vibrio cholerae and Vibrio parahaemolyticus. Ninety samples were analyzed, 45 of them collected at seafood commercial establishments and the other 45 were collected in the cultivation area. All analyses were performed according to methods of the American Public Health Association. Vibrio cholerae, V. parahaemolyticus and Salmonella sp. were not detected in any of the samples. Coagulase-positive staphylococci were observed in only one sample (80 CFU/g). The counts of coliforms at 35 and 45ºC indicated that samples obtained from both the cultivation area and place of sale were contaminated. E. coli was detected in 4 (9%) samples collected in the cultivation area and in 16 (35.5%) samples obtained from commercial establishments. These results indicate the need for monitoring the quality of raw oysters, including the implantation of programs for good mollusk manipulation and management practices.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Microbiological quality of oysters (Crassostrea gigas) produced and commercialized in the coastal region of Florianópolis - Brazil

Pereira¹,

Nunes²,

Nuernberg³

et al. 2006

Braz. J. Microbiol.

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“…According to Ayulo, Machado and Scussel (1994), fish and mussels produced and commercialized in the state of Santa Catarina were positive for S. aureus in 20% of the analyzed samples and also 60% of the bivalve samples presented S. aureus, but only 9 out of 109 strains of S. aureus produced enteroxin, including enteroxins A (4), D (1) and B (4).…”

Section: Inoculationmentioning

confidence: 99%

Quality of mussels cultivated and commercialized in Ubatuba, SP, Brazil: monitoration Bacillus cereus and Staphylococcus aureus growth after post-harvest processing

Salán¹,

Galvão²,

Furlan³

et al. 2008

Ciênc. Tecnol. Aliment.

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“…When the methicillin sensitive S. aureus (MSSA) acquires the genes responsible for methicillin resistance, the organism causes higher morbidity and mortality in humans with prolonged period of hospitalisation and are called as methicillin resistant S. aureus (MRSA) (Datta and Huang, 2008). There are a few reports on the prevalence of S. aureus in fish and fishery products from local retail markets and imported samples (Ayulo et al, 1994;Simon and Sanjeev, 2007;Saito et al, 2011;Bujjamma and Padmavathi, 2015;Obaidat et al, 2015). MRSA and methicillin resistant coagulase negative staphylococci (MR-CoNS) are recognised as zoonotic multidrug resistant pathogens causing hospital and community acquired infections in humans as well as infections in animals (Dahms et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of methicillin resistant Staphylococcus aureus in selected seafood markets and aquaculture farms in Kerala, south-west coast of India

Murugadas

Joseph²,

Reshmi³

et al. 2016

Indian J. Fish.

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A study was carried out to understand the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in selected seafood commodities, market environment as well as in aquaculture farms in Kerala, along the south-west coast of India. Two hundred and thirty three samples comprising finfish, crustaceans, molluscs and fishery environmental samples from markets as well as aquaculture farms were collected and screened for the presence of MRSA. The S. aureus isolates obtained from the samples were checked for resistance to methicillin, oxacillin and cefoxitin by antimicrobial sensitivity test and further confirmed by oxacillin agar screening method. A multiplex PCR was employed targeting mecA, nuc and 16s rDNA genes to differentiate S. aureus and MRSA from other staphylococci. It was found that 13.4% of the samples harboured MRSA while the overall prevalence of S. aureus was found to be 36.5%. This study has clearly indicated high prevalence of methicillin resistant S. aureus strains in the seafood as well as in environmental samples from seafood markets and aquaculture farms in Kerala. The prevalence of methicillin resistant strains of staphylococci in fish indicates high risk associated with fish handlers and fish farmers. Staphylococcus aureus, a well known pathogen of hospital acquired infection and food poisoning (Khan et al., 2015), is a commensal organism of skin and mucous membrane of different species of animals as well as human beings and is not generally considered as the normal microflora of fish (Huss, 1988; Mc Carthy et al., 2012). Remarkably, 20% of individuals are persistently and 30% are transiently colonised with S. aureus in the nose (Liu, 2009). When the methicillin sensitive S. aureus (MSSA) acquires the genes responsible for methicillin resistance, the organism causes higher morbidity and mortality in humans with prolonged period of hospitalisation and are called as methicillin resistant S. aureus (MRSA) (Datta and Huang, 2008). There are a few reports on the prevalence of S. aureus in fish and fishery products from local retail markets and imported samples (Ayulo et al., 1994;Simon and Sanjeev, 2007;Saito et al., 2011;Bujjamma and Padmavathi, 2015;Obaidat et al., 2015). MRSA and methicillin resistant coagulase negative staphylococci (MR-CoNS) are recognised as zoonotic multidrug resistant pathogens causing hospital and community acquired infections in humans as well as infections in animals (Dahms et al., 2014). Resistance to methicillin is determined by mecA, encoded for low affinity penicillin binding protein PBP2A (Beck et al., 1986). Non-mecA mediated methicillin resistance in S. aureus may be due to excess production of beta-lactamase resulting in low level resistance to oxacillin called as borderline oxacillin resistant Staphylococcus aureus (BORSA) (Maalej et al., 2012) or due to presence of a novel mecA gene homologue (mecALGA251 or mecC) causing resistance to beta-lactam antibiotics (Garcia-Alvarez et al., 2011). The prevalence of MRSA in fish was first reported from Malay...

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Enterotoxigenic Escherichia coli and Staphylococcus aureus in fish and seafood from the southern region of Brazil

Cited by 80 publications

References 14 publications

Microbiological quality of oysters (Crassostrea gigas) produced and commercialized in the coastal region of Florianópolis - Brazil

Microbiological quality of oysters (Crassostrea gigas) produced and commercialized in the coastal region of Florianópolis - Brazil

Quality of mussels cultivated and commercialized in Ubatuba, SP, Brazil: monitoration Bacillus cereus and Staphylococcus aureus growth after post-harvest processing

Prevalence of methicillin resistant Staphylococcus aureus in selected seafood markets and aquaculture farms in Kerala, south-west coast of India

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