2003
DOI: 10.1128/aem.69.5.2975-2984.2003
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Enterolysin A, a Cell Wall-Degrading Bacteriocin fromEnterococcus faecalisLMG 2333

Abstract: A novel antimicrobial protein, designated enterolysin A, was purified from an Enterococcus faecalis LMG 2333 culture. Enterolysin A inhibits growth of selected enterococci, pediococci, lactococci, and lactobacilli. Antimicrobial activity was initially detected only on solid media, but by growing the bacteria in a fermentor under optimized production conditions (MRS broth with 4% [wt/vol] glucose, pH 6.5, and a temperature between 25 and 35°C), the bacteriocin activity was increased to 5,120 bacteriocin units m… Show more

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Cited by 243 publications
(201 citation statements)
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“…All of the strains characterized in this study encoded multiple bacteriocins, but the number and type of bacteriocins did not correlate with the level of BV-associated bacteria in the microbiome. However, VMC3 was the only strain that lacked a gene for a bacteriocin, similar to Enterolysin A from Enterococcus faecalis (Nilsen et al, 2003). Thus, VMC3, isolated from a subject with BV at the time of sampling, could have diminished bactericidal capacity due to diminished lactic acid production and the lack of an Enterolysin A family bacteriocin.…”
Section: Discussionmentioning
confidence: 99%
“…All of the strains characterized in this study encoded multiple bacteriocins, but the number and type of bacteriocins did not correlate with the level of BV-associated bacteria in the microbiome. However, VMC3 was the only strain that lacked a gene for a bacteriocin, similar to Enterolysin A from Enterococcus faecalis (Nilsen et al, 2003). Thus, VMC3, isolated from a subject with BV at the time of sampling, could have diminished bactericidal capacity due to diminished lactic acid production and the lack of an Enterolysin A family bacteriocin.…”
Section: Discussionmentioning
confidence: 99%
“…Class II can be subdivided into three subclasses: II.1, enterocin of the pediocin family (enterocin A, enterocin P and bacteriocin 31); II.2, enterocins synthesized without a leader peptide (enterocin L50A/B, and enterocin Q); II.3, other linear, non-pediocin-type enterocins (enterocin B). Class III enterocins includes cyclic antibacterial peptides like enterocin AS-48 and Class IV enterocins are large proteins such as enterolysin A [5]. A major portion of the bacteriocin-producing enterococci have been isolated from foods (cheese, meat, fish and vegetables), animals, and humans [6].…”
Section: Introductionmentioning
confidence: 99%
“…Bacteriocin 41 only showed bacteriocin activity against E. faecalis, which suggested that the bacL 1 -encoded protein of bacteriocin 41 was highly specific for the glycan strand of the E. faecalis cell wall. Recently, another group reported the discovery of a novel cell wall-degrading bacteriocin, which has been named enterolysin A (EnlA), in an E. faecalis strain isolated from fish (36). The bacteriocin gene enlA encodes a 343-amino-acid preprotein with a sec-dependent signal peptide of 27 amino acids.…”
Section: Discussionmentioning
confidence: 99%