Enterolobium contortisiliquum Trypsin Inhibitor (EcTI), a Plant Proteinase Inhibitor, Decreases in Vitro Cell Adhesion and Invasion by Inhibition of Src Protein-Focal Adhesion Kinase (FAK) Signaling Pathways

Paula, Cláudia Alessandra Andrade de; Coulson‐Thomas, Vivien Jane; Ferreira, Joana Gasperazzo; Maza, Paloma Korehisa; Suzuki, Érika; Nakahata, Adriana Miti; Nader, Helena B.; Sampaio, Misako U.; Oliva, Maria Luiza Vilela

doi:10.1074/jbc.m111.263996

Cited by 40 publications

(48 citation statements)

References 75 publications

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“…In general, Fabaceae seeds have high levels of protease inhibitors and these molecules have been purifi ed from several legume species (Gomes et al 2005, Migliolo et al 2010, Paula et al 2012, Chevreuil et al 2014. For example, in Pithecelobium dumosum, two trypsin inhibitors were purifi ed (Oliveira et al 2007) and in Poincianella pyramidalis seeds, a Kunitz inhibitor with insecticide effect was isolated (Guimarães et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Because of the versatility in inhibiting different enzymes, bifunctional inhibitors have been investigated to understand their mechanistic inhibitory strategies, in order to elucidate their interaction with their target enzymes (Migliolo et al 2010) and their potential applications as therapeutic drugs to treat cancer and to control pests and crop diseases in transgenic plants (Pandey et al 2007, Mosolov & Valueva 2008, Paula et al 2012, Cruz-Silva et al 2013.…”

Section: Discussionmentioning

confidence: 99%

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Partial purification of trypsin/papain inhibitors from Hymenaea courbaril L. seeds and antibacterial effect of protein fractions

Brito

Melo

Alves

et al. 2016

Hoehnea

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-(Partial purification of trypsin/papain inhibitors from Hymenaea courbaril L. seeds and antibacterial effect of protein fractions). The crude extract and protein fractions of Hymenaea courbaril L. seeds were investigated for the presence of trypsin and papain inhibitors and antimicrobial activity against Vibrio parahaemolyticus, Staphylococcus aureus, and Escherichia coli. Protein fractions were obtained from the crude extract after precipitation with ammonium sulfate into three saturation ranges (0-30%, 30-60%, and 60-90%), called Hc030, Hc3060, and Hc6090, respectively. The crude extract and protein fractions inhibited trypsin and papain activity, but to different degrees. Antimicrobial activity was observed in Hc030 and Hc3060 fractions, but only against V. parahaemolyticus.The inhibitor isolated from the Hc3060 fraction was more effective in inhibiting trypsin (100% inhibition) than papain (54% inhibition), and showed an apparent molecular mass of 20 kDa. This study shows that H. courbaril seeds contain proteins with protease-inhibiting and antibacterial activity, indicating that this species is a source of bioactive compounds. Keywords: antimicrobial, cystatin, Fabaceae, protease RESUMO -(Purificação parcial de um inibidor de tripsina/papaína de sementes de Hymenaea courbaril L. e atividade antibacteriana de suas frações proteicas). O extrato bruto e frações proteicas de sementes de Hymenaea courbaril foram investigados para a presença de inibidores de tripsina e papaína e atividade antimicrobiana contra Vibrio parahaemolyticus, Staphylococcus aureus e Escherichia coli. As frações foram obtidas após precipitação do extrato bruto com sulfato de amônio em três faixas de saturação (0-30%, 30-60% e 60-90%) e denominadas Hc030, Hc3060 e Hc6090, respectivamente. O extrato bruto e as frações apresentaram diferente especificidade em inibir a atividade da tripsina e papaína. A atividade antimicrobiana foi observada nas frações Hc030 e Hc3060 e somente contra V. parahaemolyticus. O inibidor isolado a partir de Hc3060 foi mais eficiente na inibição da tripsina (100% de inibição) do que da papaína (54% de inibição), e mostrou uma massa molecular aparente de 20 kDa. Este estudo mostra que as sementes de H. courbaril possuem inibidores de proteases e proteínas com atividade antibacteriana, indicando que a espécie é uma fonte de compostos bioativos.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Partial purification of trypsin/papain inhibitors from Hymenaea courbaril L. seeds and antibacterial effect of protein fractions

Brito

Melo

Alves

et al. 2016

Hoehnea

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“…As with E. cyclocarpum, its fruits are smooth, shiny, indehiscent and deep brown (Rocha and Aguilar, 2001a) and the seeds are hard, ovoid and brown (Janzen, 1982). Recently, it was discovered that a proteinase inhibitor of this species, Enterolobium contortisiliquum trypsin inhibitor (EcTI), inhibited the invasion of gastric cancer cells, providing a possible candidate for therapy against tumors (Paula et al, 2012). This tree has been removed from natural areas owing to the advance of cattle grazing and agriculture in Brazilian SDTFs due to its poisonous fruits.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and structure of the tree Enterolobium contortisiliquum (Fabaceae) associated with remnants of a seasonally dry tropical forest

Moreira

Brandão

Araújo

et al. 2015

Flora - Morphology, Distribution, Functional Ecology of Plants

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a b s t r a c tFragmentation of tropical forests is one of the major threats to biodiversity and viable natural populations. Brazilian seasonally dry tropical forests (SDTF) are endangered because of human occupation, conversion of lands to agriculture and high deforestation rates in these fertile soils. Enterolobium contortisiliquum has been removed from SDTF natural areas due to the advance of cattle grazing and agriculture in Brazilian SDTFs. To aid conservation efforts of this species we used molecular markers to study the genetic diversity and population structure of E. contortisiliquum in Brazilian SDTF remnants. A total of 263 individuals in 13 forest patches were analyzed with 103 ISSR fragments. In spite of being found scattered among the SDTF patches, E. contortisiliquum populations exhibited high genetic diversity (mean Shannon's index of diversity = 0.384; mean genetic diversity = 0.280) and genetic divergence between populations was detected (˚S T = 0.155, p < 0.0001). UPGMA dendrogram, principal coordinate analysis and Bayesian analysis showed that E. contortisiliquum populations were clustered into three groups that were not related to geographic distance (r = 0.119, p = 0.197). However, these populations are spatially structured into groups distributed in the West, Central and East of the study area. This genetic structure may be related with landscape and both the Espinhaç o Range and São Francisco River may act as a partial geographic barrier limiting the gene flow. Because the highest rates of genetic diversity were found outside of conservation units, we propose the creation of protected areas in different geographic regions that include E. contortisiliquum populations from different genetic clusters.

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“…Integrins play a central role in these processes (12)(13)(14). Integrins are heterodimeric receptors that are formed by noncovalently associated ␣ and ␤ subunits (10).…”

mentioning

confidence: 99%

The Impaired Viability of Prostate Cancer Cell Lines by the Recombinant Plant Kallikrein Inhibitor

Ferreira

Diniz

Paula

et al. 2013

Journal of Biological Chemistry

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Background: Kallikreins play a pivotal role in establishing prostate cancer. Results: In contrast to the classical Kunitz plant inhibitor SbTI, the recombinant kallikrein inhibitor (rBbKIm) led to prostate cancer cell death, whereas fibroblast viability was not affected. Conclusion: rBbKIm shows selective cytotoxic effect and angiogenesis inhibition against prostate cancer cells. Significance: New actions of rBbKIm may contribute to understanding the mechanisms of prostate cancer.

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Enterolobium contortisiliquum Trypsin Inhibitor (EcTI), a Plant Proteinase Inhibitor, Decreases in Vitro Cell Adhesion and Invasion by Inhibition of Src Protein-Focal Adhesion Kinase (FAK) Signaling Pathways

Cited by 40 publications

References 75 publications

Partial purification of trypsin/papain inhibitors from Hymenaea courbaril L. seeds and antibacterial effect of protein fractions

Partial purification of trypsin/papain inhibitors from Hymenaea courbaril L. seeds and antibacterial effect of protein fractions

Genetic diversity and structure of the tree Enterolobium contortisiliquum (Fabaceae) associated with remnants of a seasonally dry tropical forest

The Impaired Viability of Prostate Cancer Cell Lines by the Recombinant Plant Kallikrein Inhibitor

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