The Impaired Viability of Prostate Cancer Cell Lines by the Recombinant Plant Kallikrein Inhibitor

Ferreira, Joana Gasperazzo; Diniz, Paula Malloy Motta; Paula, Cláudia Alessandra Andrade de; Lobo, Yara Aparecida; Paredes‐Gamero, Edgar Julian; Paschoalin, Thaysa; Nogueira‐Pedro, Amanda; Maza, Paloma Korehisa; Toledo, Marcos S.; Suzuki, Érika; Oliva, Maria Luíza Vilela

doi:10.1074/jbc.m112.404053

Cited by 21 publications

(13 citation statements)

References 66 publications

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“…Many of those naturally found PIs were further characterized from different plant species which mainly included trypsin from serine protease group which have been tested for various diseases (Richardson, 1991; Tamir et al, 1996; Majumdar, 2013). This review explains about PIs of all earlier reported plant species that have been used as therapeutic agents and tested against different diseases and human disorders (Table 1; Murugesan et al, 2001; Neuhof et al, 2003; Troncoso et al, 2003; Kobayashi et al, 2004; Lanza et al, 2004; Clemente et al, 2005, 2012; Kim et al, 2005; Suzuki et al, 2005; Capaldi et al, 2007; Banerjee et al, 2008; Tochi et al, 2008; Caccialupi et al, 2010; Hsieh et al, 2010; Joanitti et al, 2010; García-Gasca et al, 2012; Magee et al, 2012; de Paula et al, 2012a; Borodin et al, 2013; Ferreira et al, 2013; Rakashanda et al, 2013b; Clemente and Arques, 2014; Souza et al, 2014) and future scope to search for new species, which are as follows:…”

Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 99%

“…Kallikreins play an important role in the establishment of a common prostate cancer. RGD/RGE motifs of the inhibitor BrTI from Bauhinia rufa is included into rBbKIm to form the recombinant B. bauhinioides kallikreins (Ferreira et al, 2013). This rBbKIm inhibited trypsin (K i app = 1.6 nM), chymotrypsin (K i app = 7.4 nM), and human plasma kallikrein (K i app = 3.6 nM).…”

Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 99%

“…This rBbKIm inhibited trypsin (K i app = 1.6 nM), chymotrypsin (K i app = 7.4 nM), and human plasma kallikrein (K i app = 3.6 nM). Also, it was reported that the viability of fibroblasts was not affected when rBbKIm inhibited the cell viability of DU145 and PC3 prostate cancer cells (Ferreira et al, 2013). …”

Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 99%

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Plant Protease Inhibitors in Therapeutics-Focus on Cancer Therapy

2016

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Plants are known to have many secondary metabolites and phytochemical compounds which are highly explored at biochemical and molecular genetics level and exploited enormously in the human health care sector. However, there are other less explored small molecular weight proteins, which inhibit proteases/proteinases. Plants are good sources of protease inhibitors (PIs) which protect them against diseases, insects, pests, and herbivores. In the past, proteinaceous PIs were considered primarily as protein-degrading enzymes. Nevertheless, this view has significantly changed and PIs are now treated as very important signaling molecules in many biological activities such as inflammation, apoptosis, blood clotting and hormone processing. In recent years, PIs have been examined extensively as therapeutic agents, primarily to deal with various human cancers. Interestingly, many plant-based PIs are also found to be effective against cardiovascular diseases, osteoporosis, inflammatory diseases and neurological disorders. Several plant PIs are under further evaluation in in vitro clinical trials. Among all types of PIs, Bowman-Birk inhibitors (BBI) have been studied extensively in the treatment of many diseases, especially in the field of cancer prevention. So far, crops such as beans, potatoes, barley, squash, millet, wheat, buckwheat, groundnut, chickpea, pigeonpea, corn, and pineapple have been identified as good sources of PIs. The PI content of such foods has a significant influence on human health disorders, particularly in the regions where people mostly depend on these kind of foods. These natural PIs vary in concentration, protease specificity, heat stability, and sometimes several PIs may be present in the same species or tissue. However, it is important to carry out individual studies to identify the potential effects of each PI on human health. PIs in plants make them incredible sources to determine novel PIs with specific pharmacological and therapeutic effects due to their peculiarity and superabundance.

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Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 99%

Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 99%

Section: Roles Of Plant Protease Inhibitors In Health and Disease Conmentioning

confidence: 99%

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Plant Protease Inhibitors in Therapeutics-Focus on Cancer Therapy

2016

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“…It is a physiological process where epithelial cells proliferate to promote neovascularization. In diabetes patients, neovascularization impairs improvement in diabetic foot ulcers and causes infertility due to delayed endometrium maturation [17,18]. Heparin can have an inhibitory effect on the angiogenesis process [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Effects of Enoxaparin Emulsion on Dimethylbenzanthracene-induced Breast Cancer in Female Rats

Bolandpayeh

Hassanpour-Ezzati

Mousavi

2018

RMM

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Introduction: Enoxaparin is an anticoagulant medication. Anticoagulation inhibits tumor cell-mediated release of angiogenic proteins and diminishes angiogenic response. Angiogenesis is an important event in various cancers such as breast cancer. Angiogenesis provide oxygen and nutrients to tumor cells and causes tumor progression. The aim of the present study was to evaluate the anti-angiogenesis effect of an enoxaparin cream on breast cancer induced by dimethylbenzanthracene in rats. Methods: In this experimental in vivo study, 50 Wistar female rats were divided into negative control (vehicle), positive control (cream base), and 3 groups with enoxaparin treatment (40, 60, and 80 mg/ml). After one month of treatment along with breast cancer induction by dimethylbenzanthracene, breast tissue samples were isolated and stained with hematoxylin-eosin, and tumor growth suppression rate was calculated. Tumor size (length and width) was measured using a clipper, and the tumor volume was calculated using the following formula: V = (L × W × W)/2, where V is tumor volume, W is tumor width, L is tumor length. The data were analyzed using one-way ANOVA and Tukey’s post hoc test. Results: Tumor suppression was significantly increased in enoxaparin treatment groups compared to the positive control group (40 mg/ml of enoxaparin treated versus positive control group; P = 0.017, 60 mg/ml of enoxaparin treated versus positive control; P = 0.015, 40 mg/ml of enoxaparin treated versus positive control; P = 0.009, 60 mg/ml of enoxaparin treated versus 40 mg/ml of enoxaparin treated; P = 0.019, and 80 mg/ml of enoxaparin treated versus 40 mg/ml of enoxaparin treated; P = 0.011 in a dose-dependent manner. Conclusion: Enoxaparin inhibits breast cancer in a dose-dependent manner. The application of enoxaparin cream in patients with breast cancer may considerably reduce tumor growth.

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“…In recent years, the physiological functions and medicinal properties of buckwheat have been widely studied. However, the studies on buckwheat proteins, especially on buckwheat protease inhibitors, have mainly focused on their antitumor activities (Cui et al, 2013;Ferreira et al, 2013;Liu et al, 2001). It has been well known that buckwheat can reduce levels of blood sugar, lower cholesterol, and protect against heart disease (Hosaka et al, 2014;Lee et al, 2012;Stringer et al, 2013), partly due to its high level of protease inhibitors.…”

Section: Introductionmentioning

confidence: 99%