Enterocin T, a novel class IIa bacteriocin produced by Enterococcus sp. 812

Abstract

Enterococcus sp. 812, isolated from fresh broccoli, was previously found to produce a bacteriocin active against a number of Gram-positive bacteria, including Listeria monocytogenes. Bacteriocin activity decreased slightly after autoclaving (121 °C for 15 min), but was inactivated by protease K. Mass spectrometry analysis revealed the bacteriocin mass to be approximately 4,521.34 Da. N-terminal amino acid sequencing yielded a partial sequence, NH2-ATYYGNGVYXDKKKXWVEWGQA, by Edman degradation, which contained the consensus class IIa bacteriocin motif YGNGV in the N-terminal region. The obtained partial sequence showed high homology with some enterococcal bacteriocins; however, no identical peptide or protein was found. This peptide was therefore considered to be a novel bacteriocin produced by Enterococcus sp. 812 and was termed enterocin T.

Biosynthesis, classification, properties, and applications of Weissella bacteriocins

“…Ultra-purification was achieved using pre-equilibrated Sepharose or Sephadex columns and purity check using methods like HPLC or LCMS as per the requirement (Wu et al, 2015 ). After the completion of purification, application-based approaches have been used for food-grade development toward food safety and similar processed products (Pal and Ramana, 2010 ; Papagianni and Papamichael, 2011 ; Chen et al, 2013 ; Bancalari et al, 2020 ).…”

Detection and Characterization of Weissellicin 110, a Bacteriocin Produced by Weissella cibaria

“…The harvested cell-free supernatant was desalted and purified using a C 18 cartridge (Sep-Pak C 18 ; Waters, Milford, MA, USA) following Chen et al (17). Antimicrobial activity of the peptide fractions was tested using agar-spot diffusion assay (18).…”

Characterization of a Bacteriocin Produced by Enterococcus gallinarum CRL 1826 Isolated from Captive Bullfrog: Evaluation of its Mode of Action against Listeria monocytogenes and Gram-Negatives

“…Enterocins are bacteriocins produced by Enterococcus species that are able to inhibit Gram-positives such as L. monocytogenes [18][19][20][21][22][23] and some Gram-negatives in a lesser degree [24][25][26]. associated with L. catesbeianus hatchery environments [4,6,27].…”