Detection and Characterization of Weissellicin 110, a Bacteriocin Produced by Weissella cibaria

Wu, Heng; Srionnual, Sirinat; Yanagida, Fujitoshi; Chen, Yisheng

doi:10.15171/ijb.1159

Cited by 3 publications

(3 citation statements)

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“…We showed that this putative bacteriocin is larger (17 kDa) in size and when combined with EDTA inhibits the growth of the two indicator strains, E. coli and Salmonella enterica , at both the early and later logarithmic phase of growth ( Tenea et al, 2020b ). Previous research indicated that W. cibaria 110 produce a class IId bacteriocin, weissellicin 110 ( Wu et al, 2015 ), while the strains SP7 and SP19 harbored colicin V bacteriocin ( Patrone et al, 2020 ). The genome information presented here will help further specific studies of this strain and exploit its probiotic and antimicrobial potential.…”

Section: Resultsmentioning

confidence: 99%

Next-Generation Sequencing for Whole-Genome Characterization of Weissella cibaria UTNGt21O Strain Originated From Wild Solanum quitoense Lam. Fruits: An Atlas of Metabolites With Biotechnological Significance

Tenea

Hurtado

2021

Front. Microbiol.

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The whole genome of Weissella cibaria strain UTNGt21O isolated from wild fruits of Solanum quitoense (naranjilla) shrub was sequenced and annotated. The similarity proportions based on the genus level, as a result of the best hits for the entire contig, were 54.84% with Weissella, 6.45% with Leuconostoc, 3.23% with Lactococcus, and 35.48% no match. The closest genome was W. cibaria SP7 (GCF_004521965.1) with 86.21% average nucleotide identity (ANI) and 3.2% alignment coverage. The genome contains 1,867 protein-coding genes, among which 1,620 were assigned with the EggNOG database. On the basis of the results, 438 proteins were classified with unknown function from which 247 new hypothetical proteins have no match in the nucleotide Basic Local Alignment Search Tool (BLASTN) database. It also contains 78 tRNAs, six copies of 5S rRNA, one copy of 16S rRNA, one copy of 23S rRNA, and one copy of tmRNA. The W. cibaria UTNGt21O strain harbors several genes responsible for carbohydrate metabolism, cellular process, general stress responses, cofactors, and vitamins, conferring probiotic features. A pangenome analysis indicated the presence of various strain-specific genes encoded for proteins responsible for the defense mechanisms as well as gene encoded for enzymes with biotechnological value, such as penicillin acylase and folates; thus, W. cibaria exhibited high genetic diversity. The genome characterization indicated the presence of a putative CRISPR-Cas array and five prophage regions and the absence of acquired antibiotic resistance genes, virulence, and pathogenic factors; thus, UTNGt21O might be considered a safe strain. Besides, the interaction between the peptide extracts from UTNGt21O and Staphylococcus aureus results in cell death caused by the target cell integrity loss and the release of aromatic molecules from the cytoplasm. The results indicated that W. cibaria UTNGt21O can be considered a beneficial strain to be further exploited for developing novel antimicrobials and probiotic products with improved technological characteristics.

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Section: Resultsmentioning

confidence: 99%

Next-Generation Sequencing for Whole-Genome Characterization of Weissella cibaria UTNGt21O Strain Originated From Wild Solanum quitoense Lam. Fruits: An Atlas of Metabolites With Biotechnological Significance

Tenea

Hurtado

2021

Front. Microbiol.

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“…From 2014 up to date, further bacteriocins have been discovered in these bacteria. In particular, weissellicin 110 produced by a strain isolated from the yan-dong-gua (fermented wax gourd), namely, W. cibaria 860106, was characterized by Wu et al ( 2015 ). The bacteriocin A3 produced by W. confusa A3, a strain of dairy origin, was isolated and characterized by Goh and Philip ( 2015 ).…”

Section: An Update Of the Probiotic Potential Of Weissella ...mentioning

confidence: 99%

The Weissella and Periweissella genera: up-to-date taxonomy, ecology, safety, biotechnological, and probiotic potential

Fusco,

Chieffi,

Fanelli

et al. 2023

Front. Microbiol.

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Bacteria belonging to the genera Weissella and Periweissella are lactic acid bacteria, which emerged in the last decades for their probiotic and biotechnological potential. In 2015, an article reviewing the scientific literature till that date on the taxonomy, ecology, and biotechnological potential of the Weissella genus was published. Since then, the number of studies on this genus has increased enormously, several novel species have been discovered, the taxonomy of the genus underwent changes and new insights into the safety, and biotechnological and probiotic potential of weissellas and periweissellas could be gained. Here, we provide an updated overview (from 2015 until today) of the taxonomy, ecology, safety, biotechnological, and probiotic potential of these lactic acid bacteria.

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“…In particular W. cibaria , this species is a key member of the genus and exhibits a variety of traits and abilities. For instance, W. cibaria can produce bacteriocins such as weissellicin 110, which has strong activity against Gram-positive bacteria ( Srionnual et al, 2007 ; Wu et al, 2015 ). Some W. cibaria strains were reported to encode and harbor several genes with useful biotechnological properties ( Carlosama Adriana et al, 2021 ; Tenea and Hurtado, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Complete Genome Sequence of Weissella cibaria NH9449 and Comprehensive Comparative-Genomic Analysis: Genomic Diversity and Versatility Trait Revealed

et al. 2022

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Lactic acid bacteria (LAB) in the genus Weissella spp. contain traits in their genome that confer versatility. In particular, Weissella cibaria encodes several beneficial genes that are useful in biotechnological applications. The complete genome of W. cibaria NH9449 was sequenced and an in silico comparative analysis was performed to gain insight into the genomic diversity among members of the genus Weissella. A total of 219 Weissella genomes were used in a bioinformatics analysis of pan-genomes, phylogenetics, self-defense mechanisms, virulence factors, antimicrobial resistance, and carbohydrate-active enzymes. These investigations showed that the strain NH9449 encodes several restriction-modification-related genes and a CRISPR-Cas region in its genome. The identification of carbohydrate-active enzyme-encoding genes indicated that this strain could be beneficial in biotechnological applications. The comparative genomic analysis reveals the very high genomic diversity in this genus, and some marked differences in genetic variation and genes among Weissella species. The calculated average amino acid identity (AAI) and phylogenetic analysis of core and accessory genes shows the possible existence of three new species in this genus. These new genomic insights into Weissella species and their biological functions could be useful in the food industry and other applications.

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Detection and Characterization of Weissellicin 110, a Bacteriocin Produced by Weissella cibaria

Cited by 3 publications

References 20 publications

Next-Generation Sequencing for Whole-Genome Characterization of Weissella cibaria UTNGt21O Strain Originated From Wild Solanum quitoense Lam. Fruits: An Atlas of Metabolites With Biotechnological Significance

Next-Generation Sequencing for Whole-Genome Characterization of Weissella cibaria UTNGt21O Strain Originated From Wild Solanum quitoense Lam. Fruits: An Atlas of Metabolites With Biotechnological Significance

The Weissella and Periweissella genera: up-to-date taxonomy, ecology, safety, biotechnological, and probiotic potential

Complete Genome Sequence of Weissella cibaria NH9449 and Comprehensive Comparative-Genomic Analysis: Genomic Diversity and Versatility Trait Revealed

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