Enterobacter mori sp. nov., associated with bacterial wilt on Morus alba L.

Abstract

Two isolates of mulberry-pathogenic bacteria isolated from diseased mulberry roots were investigated in a polyphasic taxonomic study. Comparative 16S rRNA gene sequence analysis combined with rpoB gene sequence analysis allocated strains R18-2(T) and R3-3 to the genus Enterobacter, with Enterobacter asburiae, E. amnigenus, E. cancerogenus, E. cloacae subsp. cloacae, E. cloacae subsp. dissolvens and E. nimipressuralis as their closest relatives. Cells of the isolates were Gram-negative, facultatively anaerobic rods, 0.3-1.0 µm wide and 0.8-2.0 µm long, with peritrichous flagella, showing a DNA G+C content of 55.1 ± 0.5 mol%. Calculation of a similarity index based on phenotypic features and fatty acid methyl ester (FAME) analysis suggested that these isolates are members of E. cancerogenus or E. asburiae or a closely related species. Biochemical data revealed that the isolates could be differentiated from their nearest neighbours by the presence of lysine decarboxylase activity and their ability to utilize d-arabitol. DNA-DNA relatedness also distinguished the two isolates from phylogenetically closely related Enterobacter strains. Based on these data, it is proposed that the isolates represent a novel species of the genus Enterobacter, named Enterobacter mori sp. nov. The type strain is R18-2(T) ( = CGMCC 1.10322(T) = LMG 25706(T)).

New report of additional enterobacterial species causing wilt in West Bengal, India

“…However, the use of 16S rRNA gene sequence alone for identification has been questioned especially in the Enterobacteriaceae (Dahllöf et al 2000) due to various reasons, including multiple heterogeneous copies of the 16S rRNA gene within a genome (Crosby and Criddle 2003;Case et al 2007). The use of alternative core housekeeping genes, such as the RNA polymerase subunit gene (rpoB) (Zhu et al 2011;Yabuchhi et al 1996), is hence suggested for further identification of these isolates.…”

Detection of AI-2 Receptors in Genomes of Enterobacteriaceae Suggests a Role of Type-2 Quorum Sensing in Closed Ecosystems

“…In plant-associated bacteria that are not N 2 -fixing symbionts, the genes encoding the Lsr receptor complex could be identified only in the genomes of the mulberry-pathogen Enterobacter mori [89], in the plant growth-promoting endophyte Enterobacter sp. 638 [90], and in Enterobacter cancerogenus , which was originally isolated from poplars ( Populus spp.)…”

Enterobacter xiangfangensis sp. nov., isolated from Chinese traditional sourdough, and reclassification of Enterobacter sacchari Zhu et al. 2013 as Kosakonia sacchari comb. nov.

“…FAMEs were extracted and prepared according to the protocol of Sasser (1990). As described by Zhu et al (2011) and Brady et al (2013), major fatty acids of species of the genus Enterobacter are C 16 : 0 , C 17 : 0 cyclo and C 18 : 1 v7c. Comparative fatty acid compositions of strain 10-17 T and phylogenetically related reference strains are given in Table 3.…”

“…nov., with the type strain SP1 T . Strains: 1, 10-17 T (this study; n51); 2, Leclercia adecarboxylata (data from Tamura et al, 1986;n586);3, Enterobacter hormaechei (O'Hara et al, 1989;n523); 4, Enterobacter cancerogenus (Dickey & Zumoff, 1988;n53); 5, Enterobacter asburiae (Brenner et al, 1986;n568); 6, Enterobacter ludwigii (Hoffmann et al, 2005a;n516); 7, Enterobacter mori (Zhu et al, 2011;n52); 8, Enterobacter cloacae subsp. cloacae (Hoffmann et al, 2005b;n510).…”