Enterobacter xiangfangensis sp. nov., isolated from Chinese traditional sourdough, and reclassification of Enterobacter sacchari Zhu et al. 2013 as Kosakonia sacchari comb. nov.

Abstract

A Gram-stain-negative bacterial strain, 10-17T, was isolated from traditional sourdough in Heilongjiang Province, China. The bacterium was characterized by a polyphasic approach, including 16S rRNA gene sequence analysis, RNA polymerase β subunit (rpoB) gene sequence analysis, DNA gyrase (gyrB) gene sequence analysis, initiation translation factor 2 (infB) gene sequence analysis, ATP synthase β subunit (atpD) gene sequence analysis, fatty acid methyl ester analysis, determination of DNA G+C content, DNA–DNA hybridization and an analysis of phenotypic features. Strain 10-17T was phylogenetically related to Enterobacter hormaechei CIP 103441T, Enterobacter cancerogenus LMG 2693T, Enterobacter asburiae JCM 6051T, Enterobacter mori LMG 25706T, Enterobacter ludwigii EN-119T and Leclercia adecarboxylata LMG 2803T, having 99.5 %, 99.3 %, 98.7 %, 98.5 %, 98.4 % and 98.4 % 16S rRNA gene sequence similarity, respectively. On the basis of polyphasic characterization data obtained in the present study, a novel species, Enterobacter xiangfangensis sp. nov., is proposed and the type strain is 10-17T ( = LMG 27195T = NCIMB 14836T = CCUG 62994T). Enterobacter sacchari Zhu et al. 2013 was reclassified as Kosakonia sacchari comb. nov. on the basis of 16S rRNA, rpoB, gyrB, infB and atpD gene sequence analysis and the type strain is strain SP1T( = CGMCC 1.12102T = LMG 26783T).

Enterobacter bugandensis sp. nov., isolated from neonatal blood

“…Fatty acids were extracted from colonies grown after incubation for 24 h at 30 8C on nutrient agar (Gu et al, 2014). Peaks were compared against the TSBA40 library version 4.10.…”

“…Peaks were compared against the TSBA40 library version 4.10. The total fatty acid profiles obtained were compared with those of the type strains of the most closely related Enterobacter species determined under identical experimental conditions as described previously (Gu et al, 2014;Kämpfer et al, 2015). A comparison of the fatty acid composition of the strains is given in Table S4.…”

“…lignolyticus'. As a result of taxonomic conflicts, 20 species have been transferred from the genus Enterobacter to other genera (Table S1) (Brady et al, 2013;Gu et al, 2014;Oren & Garrity, 2014;Stephan et al, 2014). Members of the genus Enterobacter have been isolated as environmental organisms, and as plant and human pathogens.…”

Diazotrophic bacteria associated to sugarcane varieties cropped at Northeast Region of Brazil

“…Table 2. Characterization of isolates in groups based on colony morphology and growth on semi-solid medium and their taxonomic identification based on 16S rRNA blast analysis into the new genus Kosakonia (Gu et al, 2014). The type strain SP1T of this species was isolated from stems of sugarcane cultivar GT11 in China and its genome was completely sequenced by Chen et al (2014).…”

Reclassification of Enterobacter oryziphilus and Enterobacter oryzendophyticus as Kosakonia oryziphila comb. nov. and Kosakonia oryzendophytica comb. nov.

“…Since 2013, 12 members (Enterobacter nimipressuralis, Enterobacter amnigenus, Enterobacter gergoviae, Enterobacter pyrinus, Enterobacter cowanii, Enterobacter radicincitans, Enterobacter oryzae, Enterobacter arachidis, Enterobacter helveticus, Enterobacter pulveris, Enterobacter turicensis and Enterobacter sacchari) of the genus Enterobacter have been transferred to five novel genera (Lelliottia, Pluralibacter, Kosakonia, Franconibacter and Siccibacter) by Brady et al (2013), Gu et al (2014) and Stephan et al (2014).…”