Enterobacter cloacae harbouring blaKPC-2 and qnrB-1 isolated from a cystic fibrosis patient: a case report

Alves, D.P.; Carvalho-Assef, Ana Paula D’A.; Neto, Orlando Carlos da Conceição; Aires, Caio Augusto Martins; Albano, Rodolpho Mattos; Folescu, Tânia Wrobel; Ornelas, S.C.S.; Leão, Robson Souza; Marques, Elizabeth Andrade

doi:10.1016/j.nmni.2018.06.010

Cited by 3 publications

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References 4 publications

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“…Further, the authors found that the bla KPC gene was detected in 80 % of the isolates, corroborating the data in this study, where we found an occurrence of 88.8 % for the bla KPC gene. These occurrence rates of the bla KPC gene identified in this study highlight the persistence of this gene in K. pneumoniae , and since its detection in 2006 from Recife, Brazil 13 , it remains the main carbapenemase associated with carbapenem-resistant K. pneumoniae samples in several Brazilian states, including Recife-PE 1 , 5 , 32 .…”

Section: Discussionsupporting

confidence: 58%

“…The excessive and indiscriminate use of beta-lactam antimicrobials has culminated in the emergence of antibiotic-resistant K. pneumoniae strains and other carbapenem-resistant Enterobacteria 1 , 5 , 6 , 7 . The development of resistance is related to the production of beta-lactamases which is mediated by conjugative plasmids 8 , 9 , 10 , 11 , 12 .…”

Section: Introductionmentioning

confidence: 99%

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High plasmid variability, and the presence of IncFIB, IncQ, IncA/C, IncHI1B, and IncL/M in clinical isolates of Klebsiella pneumoniae with bla KPC and bla NDM from patients at a public hospital in Brazil.

Oliveira

Beltrão

Scavuzzi

et al. 2020

Rev. Soc. Bras. Med. Trop.

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Introduction: Antibiotic resistance in carbapenemase-producing Klebsiella pneumoniae is acquired and disseminated mainly by plasmids. Therefore, we aimed to investigate the occurrence of carbapenemase genes, analyze the genetic diversity by ERIC-PCR, and examine the most common plasmid incompatibility groups (Incs) in clinical isolates of K. pneumoniae from colonization and infection in patients from a hospital in Brazil. Methods: Twenty-seven isolates of carbapenem-resistant K. pneumoniae were selected and screened for the presence of carbapenemase genes and Incs by PCR, followed by amplicon sequencing. Results: The bla KPC and bla NDM genes were detected in 24 (88.8 %) and 16 (59.2 %) of the isolates, respectively. Thirteen isolates (48.1 %) were positive for both genes. The IncFIB (92.6 %) and IncQ (88.8 %) were the most frequent plasmids, followed by IncA/C, IncHI1B, and IncL/M, indicating that plasmid variability existed in these isolates. To our knowledge, this is the first report of IncHI1B in Brazil. We found eight isolates with clonal relationship distributed in different sectors of the hospital. Conclusions: The accumulation of resistance determinants, the variability of plasmid Incs, and the clonal dissemination detected in K. pneumoniae isolates demonstrate their potential for infection, colonization, and the dissemination of different resistance genes and plasmids.

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