Enteroaggregative E. coli O104 from an outbreak of HUS in Germany 2011, could it happen again?

Chattaway, Marie Anne; Dallman, Tim; Okeke, Iruka N.; Wain, John

doi:10.3855/jidc.2166

Cited by 57 publications

(45 citation statements)

References 65 publications

(57 reference statements)

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“…This strain was recovered from a female traveller returning from Germany who had developed hemolytic uremic syndrome and thrombotic thrombocytopenic purpura. The strain was confirmed as typical of an outbreak strain (ST678, stx-2 ositive and intimin negative) [13].…”

Section: Creation Of Reference Assemblymentioning

confidence: 89%

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Performance comparison of benchtop high-throughput sequencing platforms

Loman

Misra²,

Dallman³

et al. 2012

Nat Biotechnol

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Three bench-top high-throughput sequencing instruments are now available. The 454 GS Junior (Roche), MiSeq (Illumina) and Ion Torrent PGM (Life Technologies) are laser-printer sized and offer modest set-up and running costs. Each instrument can generate a draft bacterial genome sequence in days, making them attractive for use in the identification and characterization of pathogens in the clinical setting. We compared the performance of these instruments by sequencing isolates of Escherichia coli O104:H4 from the German outbreak of 2011. We compared performance of the platforms, analysing throughput, read length, read error profile and rate, de novo assembly quality and completeness. MiSeq had the highest throughput and lowest error rate. The 454 Junior generated the longest reads and best assemblies. The Ion Torrent PGM produced intermediate throughput with the shortest reads. The Ion Torrent PGM and 454 GS Junior both suffer from errors in homopolymers

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Section: Creation Of Reference Assemblymentioning

confidence: 89%

“…Two E. coli O104 isolates were not linked to the German outbreak and were included as comparators. Isolates were grown according to the protocol described in Chattaaway et al [13].…”

Section: Collection Of Isolatesmentioning

confidence: 99%

Performance comparison of benchtop high-throughput sequencing platforms

Loman

Misra²,

Dallman³

et al. 2012

Nat Biotechnol

Self Cite

1,212

1,001

View full text Add to dashboard Cite

show abstract

“…Most of them identified the presence of EAEC based on only one gene, when several should have been analysed given that EAEC strains can harbor different combinations of genes (Chattaway et al 2011). Most studies have focused on VT-producing EAEC O104:H4 from cattle; in contrast, we are aware of only one epidemiological study on sheep as potential faecal carriers of EAEC (Cassar et al 2004) and we have not found any such study on goats.…”

Section: Introductionmentioning

confidence: 72%

Ruminants are not a reservoir of enteroaggregative Escherichia coli

Orden

Domínguez‐Bernal

Horcajo

et al. 2017

Austral j. vet. sci.

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ABSTRACT. Enteroaggregative Escherichia coli (EAEC) causes both acute and persistent diarrhoea among children and adults in developing and developed countries. In addition, the large outbreak of food-borne illness in Europe in the summer of 2011 was caused by a verotoxin-producing EAEC O104:H4 strain. The public health threat posed by EAEC makes it important to identify animal reservoirs that might lead to epidemics in humans. Based on only one study in sheep and a few in cattle, researchers have suggested that EAEC are not found in ruminants. To expand the available data on this question, the present study applied polymerase chain reaction to faecal samples from 920 cattle, sheep and goats to detect EAEC. None of the samples tested positive for any of the EAEC genes tested, suggesting that goats, like cattle and sheep, are unlikely to be a significant reservoir of EAEC.Key words: enteroaggregative E. coli, ruminants. RESUMEN.Las Escherichia coli enteroagregativas (ECEA) causan tanto diarrea aguda como persistente en niños y adultos en países desarrollados y en vías de desarrollo. Además, el gran brote de transmisión alimentaria que sucedió en el verano de 2011 fue causado por una cepa de ECEA O104:H4 productora de verotoxinas. La amenaza a la Salud Pública debida a las ECEA hace importante identificar los reservorios animales a partir de los cuales se podrían originar epidemias o brotes en humanos. Basados en un único estudio en ovejas y unos pocos en vacas, algunos investigadores han sugerido que los ECEA no se aíslan de rumiantes. Para ampliar los datos disponibles acerca de esta cuestión, en este estudio se usó la reacción en cadena de la polimerasa para detectar ECEA en 920 muestras fecales de vacas, ovejas y cabras. Ninguna de las muestras resultó positiva a los genes de ECEA analizados, sugiriendo que las cabras, al igual que las vacas y ovejas, no son probablemente un reservorio importante de ECEA.

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“…Further information can be obtained from the detailed articles by Askar et al [8], Struelens et al [9], Frank et al [4,5], Scheutz et al [10] and from the review by Chattaway et al [3].…”

Section: Epidemiologymentioning

confidence: 99%

“…It now appears both outbreaks may have been caused by the same pathogen, Escherichia coli O104:H4 [2]. Please see the review article describing in more detail the characteristics of this bacterium by Chattaway et al [3] that is available open access in this issue of JIDC. Many challenges now face epidemiologists, microbiologists and health regulatory officials in preventing and managing these and future outbreaks of STEC 0104.…”

mentioning

confidence: 99%