Escherichia coli (STEC) serotype O104 outbreak causing haemolytic syndrome (HUS) in Germany and France

Rubino, Salvatore; Cappuccinelli, Piero; Kelvin, David J.

doi:10.3855/jidc.2172

Cited by 41 publications

(19 citation statements)

References 16 publications

(20 reference statements)

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“…Because of this, effective expression of these genes and the production of toxins is observed only after induction of the prophage, initiation of the lytic cycle and lysis of the bacteria [15]. The induction of prophages might be spontaneous or induced by external triggers.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Value of Serological Tests Against Verotoxigenic Escherichia coli in Hemolytic Uremic Syndrome in Children

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Diagnostic Value of Serological Tests Against Verotoxigenic Escherichia coli in Hemolytic Uremic Syndrome in Children

et al. 2015

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“…This assumption is supported by the following epidemiological, demographical, biological, microbiological, pathological, and clinical features of German Hemolytic Uremic Syndrome/Enterohemorrhagic Escherichia Coli (GHUSEC): high pathogenicity and extended spectrum beta-lactamase antibiotic resistance of a chimeric (enterohaemorrhagic/enteroaggregative) O104:H4 strain (1,(4)(5)(6)(7)(8)(9)(10), sudden appearance and disappearance of the pathogen (1), suitability for genetic manipulation (11), long term carriage and excretion of the pathogen (9,11,12), easy pathogen spreading by different transmission routes (9,12), longer incubation period than typical for known EHEC serotypes (1,12), and no definite conclusions about the use of antibiotics in acute EHEC diarrhoea as well as for the causal therapy at all (2). There was a rapidly increasing number of patients with severe diarrhoea and unusually high proportion of patients with HUS, very fast supra-regional spread throughout Germany and in 13 other European countries plus cases in the USA and Canada and unexpectedly high incidence in adults and females (13)(14)(15). The causative role of fenugreek seeds exported from Egypt was questionable (12) due to difficulties to isolate the pathogen from fenugreek seeds and to identify sources of infection, reservoirs and modus of contamination of the food (1).…”

Section: Introductionmentioning

confidence: 96%

Analysis of Escherichia Coli O104:H4 Outbreak in Germany in 2011 Using Differentiation Method for Unusual Epidemiological Events

Radosavljević¹,

Finke²,

Belojević

2016

Cent Eur J Public Health

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SUMMARYAim: The aim of the study was to further clarify the origin of Escherichia coli O104:H4 outbreak in Germany in 2011 (German Ec) as the likelihood of a deliberate act has not been excluded in previous analyses.Methods : We use an original and the most detailed scoring method so far, with 33 parameters pertaining to the source of infection/reservoir or possible perpetrator, pathogen or biological agent, transmission mechanism/factors or means/media of delivery, and population at risk or target.Results: Total scores for a deliberate or accidental epidemic indicate that the outbreak was more probably caused unintentionally, presumably due to technical accidents or hygienic shortcomings in the food chain.Conclusions: The validity of the present assessment is limited by the lack of data on the reservoir of the pathogen, the source of infection, and the mode of food contamination. Conclusive evidences on these parameters are essential for the final clarification of the outbreak origin.

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“…The locus of enterocyte effacement codes for intimin (gene eae) and other proteins implicated in attaching and effacing lesions on the intestinal mucosa. Several patho genic serotypes of STEC have been described since the 1980s and have been implicated in outbreaks and hemolyticuremic syndrome cases (9,35,61,73). However, the first STEC serotype to be isolated, E. coli 0157:H7, has been implicated in most of the foodbome outbreaks and hemolytic-uremic syndrome cases (17,19,38,55,58,68).…”

mentioning

confidence: 99%

Novel Real-Time PCR Method To Detect Escherichia coli O157:H7 in Raw Milk Cheese and Raw Ground Meat

Miszczycha

Ganet

Dunière

et al. 2012

Journal of Food Protection

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Raw milk, raw milk cheeses, and raw ground meat have been implicated in Escherichia coli O157:H7 outbreaks. Developing methods to detect these bacteria in raw milk and meat products is a major challenge for food safety. The aim of our study was to develop a real-time PCR assay to detect E. coli O157:H7 in raw milk cheeses and raw ground meat. Well-known primers targeting a mutation at position +93 of the uidA gene in E. coli O157:H7 were chosen, and a specific TaqMan-minor groove binder probe was designed. This probe targets another mutation, at position +191 of the uidA gene in E. coli O157:H7. The first step in the study was to evaluate the specificity of this probe with 156 different O157:H7/NM strains and 48 non-O157:H7/NM strains of E. coli. The sensitivity of the method was evaluated by pre- and postinoculation of cheeses and meat enrichments with different E. coli O157:H7 strains. All the E. coli O157:H7 isolates tested were positive, and none of the other bacteria were detected. Our results indicate that this method is sensitive enough to detect 10(2) E. coli O157:H7 isolates per ml of cheese or meat enrichment broth (24 h at 41.5° C) and is more sensitive than the International Organization for Standardization reference method. We can conclude that this new real-time PCR protocol is a useful tool for rapid, specific, and sensitive detection of E. coli O157:H7 in raw milk and raw ground meat products.

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Escherichia coli (STEC) serotype O104 outbreak causing haemolytic syndrome (HUS) in Germany and France

Cited by 41 publications

References 16 publications

Diagnostic Value of Serological Tests Against Verotoxigenic Escherichia coli in Hemolytic Uremic Syndrome in Children

Diagnostic Value of Serological Tests Against Verotoxigenic Escherichia coli in Hemolytic Uremic Syndrome in Children

Analysis of Escherichia Coli O104:H4 Outbreak in Germany in 2011 Using Differentiation Method for Unusual Epidemiological Events

Novel Real-Time PCR Method To Detect Escherichia coli O157:H7 in Raw Milk Cheese and Raw Ground Meat

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