Enteric methane emissions in response to ruminal inoculation of Propionibacterium strains in beef cattle fed a mixed diet

Vyas, D.; Alazzeh, Awfa Y.; McGinn, S. M.; McAllister, Tim A.; Harstad, Odd Magne; Holo, Helge; Beauchemin, K. A.

doi:10.1071/an14801

Cited by 14 publications

(18 citation statements)

References 15 publications

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“…The increases in CH 4 emissions observed with the supplementation of P. freudenreichii 53-W in our study cannot be explained by the above hypothesis as, as mentioned above, there were no changes in VFA profiles (Additional file 2: Table S2). However, in our previous study with wethers, this strain also increased CH 4 emissions (g CH 4 /kg DMI) [5] and a similar observation (increased trend in g CH 4 /kg DMI) was reported by Vyas et al [27] in beef heifers fed a mixed diet (60:40 forage to concentrate ratio on DM basis) with Propionibacterium supplementation ( P. freudenreichii T114, T54 and P. thoenii T159). In the present study, the starch level of HSD was similar to the study of Vyas et al [27].…”

Section: Resultssupporting

confidence: 84%

Bacterial direct-fed microbials fail to reduce methane emissions in primiparous lactating dairy cows

Jeyanathan

Martin

Eugène

et al. 2019

J Animal Sci Biotechnol

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Direct-fed microbials (DFM) are considered as a promising technique to improve animal productivity without affecting animal health or harming the environment. The potential of three bacterial DFM to reduce methane (CH 4 ) emissions, modulate ruminal fermentation, milk production and composition of primiparous dairy cows was examined in this study. As previous reports have shown that DFM respond differently to different diets, two contrasting diets were used in this study. Eight lactating primiparous cows were randomly divided into two groups that were fed a corn silage-based, high-starch diet (HSD) or a grass silage-based, high-fiber diet (HFD). Cows in each dietary group were randomly assigned to four treatments in a 4 × 4 Latin square design. The bacterial DFM used were selected for their proven CH 4 -reducing effect in vitro . Treatments included control (without DFM) and 3 DFM treatments: Propionibacterium freudenreichii 53-W (2.9 × 10 10 colony forming units (CFU)/cow per day), Lactobacillus pentosus D31 (3.6 × 10 11 CFU/cow per day) and Lactobacillus bulgaricus D1 (4.6 × 10 10 CFU/cow per day). Each experimental period included 4 weeks of treatment and 1 week of wash-out, with measures performed in the fourth week of the treatment period. Enteric CH 4 emissions were measured during 3 consecutive days using respiration chambers. Rumen samples were collected for ruminal fermentation parameters and quantitative microbial analyses. Milk samples were collected for composition analysis. Body weight of cows were recorded at the end of each treatment period. Irrespective of diet, no mitigating effect of DFM was observed on CH 4 emissions in dairy cows. In contrast, Propionibacterium increased CH 4 intensity by 27% (g CH 4 /kg milk) in cows fed HSD. There was no effect of DFM on other fermentation parameters and on bacterial, archaeal and protozoal numbers. Similarly, the effect of DFM on milk fatty acid composition was negligible. Propionibacterium and L. pentosus DFM tended to increase body weight gain with HSD. We conclude that, contrary to the effect previously observed in vitro , bacterial DFM Propionibacterium freudenreichii 53-W, Lactobacillus pentosus D31 and Lactobacillus bulgaricus D1 did not alter ruminal fermentation and failed to reduce CH 4 emissions in lactating primiparous cows fed high-starch or high-fiber diets. Electronic supplementary material The online version of this article (10.1186/s40104-019-0342-9) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 84%

Bacterial direct-fed microbials fail to reduce methane emissions in primiparous lactating dairy cows

Jeyanathan

Martin

Eugène

et al. 2019

J Animal Sci Biotechnol

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“…Competition for [H] with methanogenesis was therefore in their experiment controlled by enzyme kinetics, but because propionate production was not affected by addition of M. jalaludinii and succinate increased only temporarily, it is difficult to conclude which was the competing [H] sink or sinks. On the other hand, addition of propionibacteria in vivo was unsuccessful to decrease CH 4 production or increase propionate molar proportion (Aikman et al, 2011 ; Vyas et al, 2014a , b , 2015 ). Vyas et al ( 2014a ) quantified the added propionibacteria strains throughout the day and concluded that added propionibacteria did not persist long enough in the rumen to cause effects on CH 4 and propionate.…”

Section: Discussionmentioning

confidence: 99%

Limits to Dihydrogen Incorporation into Electron Sinks Alternative to Methanogenesis in Ruminal Fermentation

Ungerfeld

2015

Front. Microbiol.

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Research is being conducted with the objective of decreasing methane (CH4) production in the rumen, as methane emissions from ruminants are environmentally damaging and a loss of digestible energy to ruminants. Inhibiting ruminal methanogenesis generally results in accumulation of dihydrogen (H2), which is energetically inefficient and can inhibit fermentation. It would be nutritionally beneficial to incorporate accumulated H2 into propionate or butyrate production, or reductive acetogenesis. The objective of this analysis was to examine three possible physicochemical limitations to the incorporation of accumulated H2 into propionate and butyrate production, and reductive acetogenesis, in methanogenesis-inhibited ruminal batch and continuous cultures: (i) Thermodynamics; (ii) Enzyme kinetics; (iii) Substrate kinetics. Batch (N = 109) and continuous (N = 43) culture databases of experiments with at least 50% inhibition in CH4 production were used in this meta-analysis. Incorporation of accumulated H2 into propionate production and reductive acetogenesis seemed to be thermodynamically feasible but quite close to equilibrium, whereas this was less clear for butyrate. With regard to enzyme kinetics, it was speculated that hydrogenases of ruminal microorganisms may have evolved toward high-affinity and low maximal velocity to compete for traces of H2, rather than for high pressure accumulated H2. Responses so far obtained to the addition of propionate production intermediates do not allow distinguishing between thermodynamic and substrate kinetics control.

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“…In another study, supplementing rumen batch cultures with fumaratereducing enterococci caused large decreases in CH 4 and increases in propionate concentration . A slight decrease in CH 4 production per kilogram of ingested feed occurred when supplementing Propionibacterium strains to heifers fed a highforage (Vyas et al, 2014a), but not a mixed (Vyas et al, 2016), or a high-concentrate (Vyas et al, 2014b), diet. Rumen succinate (2011) Isolate H3HH Rumen 1390 Boccazzi and Patterson (2011) producers W. succinogenes and Mannheimia succiniciproducens could be interesting candidates to compete with methanogens at low H 2 concentrations, as they possess [NiFe]-hydrogenases for H 2 uptake (Søndergaard et al, 2016).…”

Section: The Competition For Dihydrogenmentioning

confidence: 96%

Metabolic Hydrogen Flows in Rumen Fermentation: Principles and Possibilities of Interventions

2020

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Rumen fermentation affects ruminants productivity and the environmental impact of ruminant production. The release to the atmosphere of methane produced in the rumen is a loss of energy and a cause of climate change, and the profile of volatile fatty acids produced in the rumen affects the post-absorptive metabolism of the host animal. Rumen fermentation is shaped by intracellular and intercellular flows of metabolic hydrogen centered on the production, interspecies transfer, and incorporation of dihydrogen into competing pathways. Factors that affect the growth of methanogens and the rate of feed fermentation impact dihydrogen concentration in the rumen, which in turn controls the balance between pathways that produce and incorporate metabolic hydrogen, determining methane production and the profile of volatile fatty acids. A basic kinetic model of competition for dihydrogen is presented, and possibilities for intervention to redirect metabolic hydrogen from methanogenesis toward alternative useful electron sinks are discussed. The flows of metabolic hydrogen toward nutritionally beneficial sinks could be enhanced by adding to the rumen fermentation electron acceptors or direct fed microbials. It is proposed to screen hydrogenotrophs for dihydrogen thresholds and affinities, as well as identifying and studying microorganisms that produce and utilize intercellular electron carriers other than dihydrogen. These approaches can allow identifying potential microbial additives to compete with methanogens for metabolic hydrogen. The combination of adequate microbial additives or electron acceptors with inhibitors of methanogenesis can be effective approaches to decrease methane production and simultaneously redirect metabolic hydrogen toward end products of fermentation with a nutritional value for the host animal. The design of strategies to redirect metabolic hydrogen from methane to other sinks should be based on knowledge of the physicochemical control of rumen fermentation pathways. The application of new -omics techniques together with classical biochemistry methods and mechanistic modeling can lead to exciting developments in the understanding and manipulation of the flows of metabolic hydrogen in rumen fermentation.

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Enteric methane emissions in response to ruminal inoculation of Propionibacterium strains in beef cattle fed a mixed diet

Cited by 14 publications

References 15 publications

Bacterial direct-fed microbials fail to reduce methane emissions in primiparous lactating dairy cows

Bacterial direct-fed microbials fail to reduce methane emissions in primiparous lactating dairy cows

Limits to Dihydrogen Incorporation into Electron Sinks Alternative to Methanogenesis in Ruminal Fermentation

Metabolic Hydrogen Flows in Rumen Fermentation: Principles and Possibilities of Interventions

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