Entangling Relation of Micro RNA-let7, miRNA-200 and miRNA-125 with Various Cancers

Masood, Nosheen; Yasmin, Azra

doi:10.1007/s12253-016-0184-0

Cited by 23 publications

(16 citation statements)

References 64 publications

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“…Peng et al demonstrated that the let-7b microRNA expression was positively associated with patient age and severity of lens opacity [53]. The lncRNA XIST/let-7/TGF-β regulatory network might play a role in the pathogenesis of ARC [54][55][56]. LncRNA-MIAT is shown to be specifically up-regulated both in the plasma fraction of whole blood and aqueous humour of cataract patients.…”

Section: Discussionmentioning

confidence: 99%

Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function

Liu

Shan

et al. 2018

Cell Physiol Biochem

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Background/Aims: Age-related cataract (ARC) remains the leading cause of visual impairment among the elderly population. Long non-coding RNAs (lncRNAs) have emerged as potential regulators in many ocular diseases. However, the role of lncRNAs in nuclear ARC, a subtype of ARC, requires further elucidation. Methods: LncRNA sequencing was performed to identify differentially expressed lncRNAs between the capsules of transparent and nuclear ARC lenses. Expression validation was confirmed by qRT-PCR. MTT assay, Calcein-AM and propidium iodide double staining, Rhodamine 123 and Hoechst double staining, EdU and transwell assay were used to determine the role of H19 or miR-675 in the viability, apoptosis, proliferation and migration of primary cultured human lens epithelial cells (HLECs). Bioinformatics and luciferase reporter assays were used to identify the binding target of miR-675. Results: Sixty-three lncRNAs are differentially expressed between the capsules of transparent and nuclear ARC lenses. One top abundantly expressed lncRNA, H19, is significantly up-regulated in the nuclear ARC lens capsules and positively associated with nuclear ARC grade. H19 knockdown accelerates apoptosis development and reduces the proliferation and migration of HLECs upon oxidative stress. H19 is the precursor of miR-675, and a reduction of H19 inhibits miR-675 expression. miR-675 regulates CRYAA expression by targeting the binding site within the 3’UTR. Moreover, miR-675 increases the proliferation and migration while decreasing the apoptosis of HLECs upon oxidative stress. Conclusion: H19 regulates HLECs function through miR-675-mediated CRYAA expression. This finding would provide a novel insight into the pathogenesis of nuclear ARC.

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Section: Discussionmentioning

confidence: 99%

Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function

Liu

Shan

et al. 2018

Cell Physiol Biochem

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“…It has been observed that the expression of let-7 family members is downregulated in many cancer types during tumor progression promoting cell cycle progression. A number of studies revealed in cancer patients significant let-7 family downregulation in renal cell, lung, bladder, colon, gastric, hepatocellular, breast, uterine and ovarian cancer 12 .…”

Section: Introductionmentioning

confidence: 99%

Next Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research

Guelfi

Cochetti

Stefanetti

et al. 2018

Sci Rep

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There is emerging evidence that microRNAs (miRNAs) dysregulation is involved in the genesis and the progression of Prostate Cancer (PCa), thus potentially increasing their use in urological clinical practice. This is the first pilot study which utilizes Illumina Deep Sequencing to examine the entire miRNAs spectrum existent in urine exfoliated prostate cells (UEPCs) of PCa patients. A total of 11 male patients with histological diagnosis of PCa were enrolled in the present study. First-catch urine (30 mL) was collected following a prostate massage. Total RNA was extracted from urine and sequenced using an HiSeq2500 System (Illumina). QPCR assay was used to validate the highest NGS results in PCA patients and in age-matched, caucasian men. Remarkably, PCA let-7 family was down-regulated (P < 0.01), compared to the controls. The results of our study support the notion of a relatively high diagnostic value of miRNA family for PCa detection, especially in the let-7 family. The present research confirmed the potential use of miRNAs as non-invasive biomarkers in the diagnosis of PCa, potentially reducing the invasiveness of actual clinical strategy.

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“…Along with the complementary roles of let-7 and miRNA-125 in inflammatory signaling, these miRNAs are also clustered together by their genomic location on the long arm of chromosome 21 (21q21.1) [113]. Let-7, miR-125b, and miR-200 are all involved in pathways of cancer progression, with miR-125b known to control apoptosis and cell proliferation [114]. Chang et al found that miR-125b directly binds to BMPR1B, a tumor suppressor of in ovarian cancer, and found single-nucleotide polymorphisms in this binding site that correlated with susceptibility to endometriosis [115].…”

Section: Mir-125bmentioning

confidence: 99%

MicroRNAs in endometriosis: biological function and emerging biomarker candidates†

Björkman

Taylor

2019

Biology of Reproduction

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MicroRNAs (miRNAs), a class of small noncoding RNA molecules, have been recognized as key post-transcriptional regulators associated with a multitude of human diseases. Global expression profiling studies have uncovered hundreds of miRNAs that are dysregulated in several diseases, and yielded many candidate biomarkers. This review will focus on miRNAs in endometriosis, a common chronic disease affecting nearly 10% of reproductive-aged women, which can cause pelvic pain, infertility, and a myriad of other symptoms. Endometriosis has delayed time to diagnosis when compared to other chronic diseases, as there is no current accurate, easily accessible, and noninvasive tool for diagnosis. Specific miRNAs have been identified as potential biomarkers for this disease in multiple studies. These and other miRNAs have been linked to target genes and functional pathways in disease-specific pathophysiology. Highlighting investigations into the roles of tissue and circulating miRNAs in endometriosis, published through June 2018, this review summarizes new connections between miRNA expression and the pathophysiology of endometriosis, including impacts on fertility. Future applications of miRNA biomarkers for precision medicine in diagnosing and managing endometriosis treatment are also discussed.

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Entangling Relation of Micro RNA-let7, miRNA-200 and miRNA-125 with Various Cancers

Cited by 23 publications

References 64 publications

Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function

Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function

Next Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research

MicroRNAs in endometriosis: biological function and emerging biomarker candidates†

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