Enrichment of IFN-γ producing cells in different murine adipose tissue depots upon infection with an apicomplexan parasite

Teixeira, Luzia; Marques, Raquel M.; Ferreirinha, Pedro; Bezerra, Filipa; Melo, Joana; Moreira, João; Pinto, Ana; Correia, Alexandra; Ferreira, Paula; Vilanova, Manuel

doi:10.1038/srep23475

Cited by 18 publications

(37 citation statements)

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“…Various immune cells, such as NK, NK T, and TCRγδ + cells, CD4 + and CD8 + TCRβ + lymphocytes account for the production of IFN-γ, and interferon-inducible GTPases and nitric oxide synthase are upregulated with the increase in IFN-γ production. All of these effects promote host control of intracellular parasite growth ( 54 ). In our study, WT mice infected with N. caninum produced a high level of IFN-γ in serum, while the IFN-γ production of Nlrp3 −/− , Asc −/− , and Caspase-1/11 −/− mice was significantly impaired, accompanied by severe defects in IL-18 production.…”

Section: Discussionmentioning

confidence: 99%

NLRP3 Inflammasome Participates in Host Response to Neospora caninum Infection

et al. 2018

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Neospora caninum is an intracellular protozoan parasite closely related to Toxoplasma gondii that mainly infects canids as the definitive host and cattle as the intermediate host, resulting in abortion in cattle and leading to financial losses worldwide. Commercial vaccines or drugs are not available for the prevention and treatment of bovine neosporosis. Knowledge about the hallmarks of the immune response to this infection could form the basis of important prevention strategies. The innate immune system first responds to invading parasite and subsequently initiates the appropriate adaptive immune response against this parasite. Upon infection, activation of host pattern-recognition receptors expressed by immune cells triggers the innate immune response. Toll-like receptors, NOD-like receptors, and C-type lectin receptors play key roles in recognizing protozoan parasite. Therefore, we aimed to explore the role of the NLRP3 inflammasome during the acute period of N. caninum infection. In vitro results showed that N. caninum infection of murine bone marrow-derived macrophages activated the NLRP3 inflammasome, accompanied by the release of IL-1β and IL-18, cleavage of caspase-1, and induction of cell death. K+ efflux induced by N. caninum infection participated in the activation of the inflammasome. Infection of mice deficient in NLRP3, ASC, and caspase-1/11 resulted in decreased production of IL-18 and reduced IFN-γ in serum. Elevated numbers of monocytes/macrophages and neutrophils were found at the initial infection site, but they failed to limit N. caninum replication. These findings suggest that the NLRP3 inflammasome is involved in the host response to N. caninum infection at the acute stage and plays an important role in limiting parasite growth, and it may enhance Th1 response by inducing production of IFN-γ. These findings may help devise protocols for controlling neosporosis.

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Section: Discussionmentioning

confidence: 99%

NLRP3 Inflammasome Participates in Host Response to Neospora caninum Infection

et al. 2018

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“…comparative analysis of myeloid cell populations in the adipose tissue of animals seropositive or seronegative for Neospora caninum. It has been shown in the murine model that N. caninum can impact on the frequencies of immune populations present in adipose tissue 12,53 . Since N. caninum infection is highly prevalent in Portuguese bovine herds, where seropositivity to this parasite can reach 80% 54 , we reanalysed our data splitting the animals in seropositive and seronegative to N. caninum.…”

Section: Cd45 Negative Cells In Bovine Adipose Tissue Flow Cytometrymentioning

confidence: 99%

“…from whole blood were prepared as described above and stained by a previously described protocol with some modifications 12 . Briefly, peroxidase activity was blocked by treatment with 3% hydrogen peroxide in methanol (Merck, Darmstadt, Germany) for 20 min.…”

Section: Immunocytochemistry Cytospins Of Svf Cells Isolated From Mamentioning

confidence: 99%

“…Moreover, lymphocytes have an important role in maintaining homeostasis of the adipose tissue 2,9 . On the other hand the adipose tissue can also have important contributions for the immune function 8,10 , as for example being a reservoir for memory T cells with potential to protect from infection 11,12 . The adipose tissue has also gained major interest as a source of non-immune cells, such as stem cells due to their potential in human [13][14][15] and veterinary medicine 16,17 .…”

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Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows

Oliveira

Pinto

Correia

et al. 2020

Sci Rep

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immune cells resident in adipose tissue have important functions in local and systemic metabolic homeostasis. Nevertheless, these immune cell populations remain poorly characterized in bovines. Recently, we described diverse lymphocyte subpopulations in adipose tissue of Holstein-Friesian cows. Here, we aimed at characterising myeloid cell populations present in bovine adipose tissue using multicolour flow cytometry, cell sorting and histochemistry/immunohistochemistry. Macrophages, CD14 + CD11b + MHc-ii + CD45 + cells, were identified in mesenteric and subcutaneous adipose tissue, though at higher proportions in the latter. Mast cells, identified as SSC-A high CD11b −/+ CD14 − MHcii − CH138A − CD45 + cells, were also observed in adipose tissue and found at higher proportions than macrophages in mesenteric adipose tissue. Neutrophils, presenting a CH138A + CD11b + phenotype, were also detected in mesenteric and subcutaneous adipose tissue, however, at much lower frequencies than in the blood. Our gating strategy allowed identification of eosinophils in blood but not in adipose tissue although being detected by morphological analysis at low frequencies in some animals. A population not expressing CD45 and with the CH138A + CD11b − MHc-ii − phenotype, was found abundant and present at higher proportions in mesenteric than subcutaneous adipose tissue. The work reported here may be useful for further studies addressing the function of the described cells.www.nature.com/scientificreports www.nature.com/scientificreports/ colour-flow cytometry revealed the presence of CD14 + cells, CD172a + cells, CD11c + cells and CD163 + cells in the stromal vascular fraction (SVF) of subcutaneous and omental adipose tissue 23,24 . Recently, Aylward et al. 25 reported the presence of CD172a + CD11b + cells, CD11c + MHC-II + cells, and CD11b + CD11c + cells in mesenteric adipose tissue. However, these markers are not exclusive to macrophages but common to other myeloid cell populations or can be even expressed in non-hematopoietic cells. As an example, studies in humans and mice have shown that CD14 can be expressed in non-myeloid cells 26 such as preadipocytes 27 and epithelial cells 28 . Also, CD172a is expressed by granulocytes such as eosinophils 29 and mast cells 30 beside its expression in monocytes/ macrophages. Since mast cells 31,32 and eosinophils 3,33 have already been described in adipose tissue, single markers analysis limits the interpretation of the data. Therefore, to characterize the different myeloid cell populations in bovine adipose tissue we used a multicolour flow cytometry panel associated with cell sorting and histochemistry/immunohistochemistry analysis. Our strategy allowed identification and isolation of macrophages, mast cells and neutrophils with macrophages being observed at different proportions in mesenteric adipose tissue (MAT) and subcutaneous adipose tissue (SAT). We also showed that CD45 negative cells represent more than 40% of all the cells in the adipose tissue SVF of MAT. A non-leukocyte populat...

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“…Teixeira e colaboradores evidenciaram um aumento rápido das células produtoras de IFN-γ, o qual se mantem, em tecidos adiposos distintos de camundongos infectados com N. caninum. Após o desafio com o parasita, as células inatas, tais como NK, NK T, mas também os linfócitos T CD4 + e CD8 + contribuíram para a produção de IFNγ observada desde o primeiro dia da infecção (TEIXEIRA et al, 2016). IFN-γ é responsável por criar o ambiente de citocinas adequado para a ativação da resposta imune adaptativa Th1.…”

Section: Papel Da Gal-1 Na Resposta Imune Adaptativa Contra N Caninumunclassified

Função da galectina-1 na resistência do hospedeiro contra a infecção experimental por Neospora caninum

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Enrichment of IFN-γ producing cells in different murine adipose tissue depots upon infection with an apicomplexan parasite

Cited by 18 publications

References 59 publications

NLRP3 Inflammasome Participates in Host Response to Neospora caninum Infection

NLRP3 Inflammasome Participates in Host Response to Neospora caninum Infection

Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows

Função da galectina-1 na resistência do hospedeiro contra a infecção experimental por Neospora caninum

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