Enrichment of endogenous fractalkine and anti-inflammatory cells via aptamer-functionalized hydrogels

Enam, Syed Faaiz; Krieger, Jack R.; Saxena, Tarun; Watts, Brian; Olingy, Claire; Botchwey, Edward A.; Bellamkonda, Ravi V.

doi:10.1016/j.biomaterials.2017.07.013

Cited by 32 publications

(31 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In turn, newly formed blood vessels have been shown to guide Schwann cells and hence axon regrowth across PN injuries 68 , 69 . In support of these observations, alternatively activated, anti-inflammatory (M2-like) macrophages are linked to improved PN remodeling and positive outcomes 70 and anti-inflammatory signaling can increase Schwann cell differentiation and migration 71 and axon growth 67 . Moreover, PN-specific ECM bioscaffolds can increase M2-like macrophage polarization and Schwann cell migration 72 .…”

Section: Discussionmentioning

confidence: 82%

Fetal extracellular matrix nerve wraps locally improve peripheral nerve remodeling after complete transection and direct repair in rat

Ren

Faust

Merwe

et al. 2018

Sci Rep

View full text Add to dashboard Cite

In peripheral nerve (PN) injuries requiring surgical repair, as in PN transection, cellular and ECM remodeling at PN epineurial repair sites is hypothesized to reduce PN functional outcomes by slowing, misdirecting, or preventing axons from regrowing appropriately across the repair site. Herein this study reports on deriving and analyzing fetal porcine urinary bladder extracellular matrix (fUB-ECM) by vacuum assisted decellularization, fabricating fUBM-ECM nerve wraps, and testing fUB-ECM nerve wrap biocompatibility and bioactivity in a trigeminal, infraorbital nerve (ION) branch transection and direct end-to-end repair model in rat. FUB-ECM nerve wraps significantly improved epi- and endoneurial organization and increased both neovascularization and growth associated protein-43 (GAP-43) expression at PN repair sites, 28-days post surgery. However, the number of neurofilament positive axons, remyelination, and whisker-evoked response properties of ION axons were unaltered, indicating improved tissue remodeling per se does not predict axon regrowth, remyelination, and the return of mechanoreceptor cortical signaling. This study shows fUB-ECM nerve wraps are biocompatible, bioactive, and good experimental and potentially clinical devices for treating epineurial repairs. Moreover, this study highlights the value provided by precise, analytic models, like the ION repair model, in understanding how PN tissue remodeling relates to axonal regrowth, remyelination, and axonal response properties.

show abstract

Section: Discussionmentioning

confidence: 82%

Fetal extracellular matrix nerve wraps locally improve peripheral nerve remodeling after complete transection and direct repair in rat

Ren

Faust

Merwe

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“… 73 , 74 Similarly, the ease with which terminal-amines can be introduced during oligonucleotide synthesis has led to a number of reports on EDC/NHS-mediated biomaterial-aptamer functionalization. 75 , 76 …”

Section: Chemical Conjugationmentioning

confidence: 99%

“… 199 , 348 − 351 Similarly, due to the widespread availability of the synthetic oligonucleotide phosphoramidite building block “Acrydite”, free-radical polymerization remains one of the most common methods through which to form DNA and RNA functionalized biomaterials. 76 , 352 − 356 By undertaking polymerization in the presence of a comonomer, the density of biomolecule presentation can be easily tuned, allowing potential difficulties from steric hindrance to be overcome. 342 Initiation of polymerization can be triggered by a number of means, including heat, UV and visible light (as discussed in section 7.1 ), redox reactions, and electrochemistry.…”

Section: Polymerizations Of Low Molecular Weight Monomersmentioning

confidence: 99%

“…Indeed, many of the examples of biomaterial–oligonucleotide conjugation described in this review act as a means to subsequently bind and control the release of growth factors, cytokines, and ECM proteins. 76 , 158 , 352 , 353 , 355 , 356 , 482 DNA and RNA are inherently less stable than peptides and often more expensive to access synthetically. However, the ability to access long aptamer sequences is attractive, allowing the production of constructs with increased binding affinity.…”

Section: Noncovalent Systemsmentioning

confidence: 99%

See 1 more Smart Citation

Achieving Controlled Biomolecule–Biomaterial Conjugation

2018

View full text Add to dashboard Cite

The conjugation of biomolecules can impart materials with the bioactivity necessary to modulate specific cell behaviors. While the biological roles of particular polypeptide, oligonucleotide, and glycan structures have been extensively reviewed, along with the influence of attachment on material structure and function, the key role played by the conjugation strategy in determining activity is often overlooked. In this review, we focus on the chemistry of biomolecule conjugation and provide a comprehensive overview of the key strategies for achieving controlled biomaterial functionalization. No universal method exists to provide optimal attachment, and here we will discuss both the relative advantages and disadvantages of each technique. In doing so, we highlight the importance of carefully considering the impact and suitability of a particular technique during biomaterial design.

show abstract

“…This study demonstrates that the concept of modulating cell responses by locally enriching the concentration of a given factor is plausible, without delivery of any exogenous proteins or cells. [166] Aptamers can also be useful for manipulating cell adhesion in dynamic culture systems, for example, to generate cell sheets. [178] Reversible intermolecular hybridization is particularly important for this purpose, ensuring a controllable way to activate and inactivate adhesion motifs without any harsh conditions that could affect cellular viability.…”

Section: Selective Ligands For Gf Capturementioning

confidence: 99%

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Teixeira

Domingues

Shevchuk

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

Growth factors (GFs) are proteins secreted by cells that regulate a variety of biological processes. Although they have long been proposed as potent therapeutic agents, their administration in a soluble form has proven costly and ineffective due to their short half-lives in biological environments. Biomaterial-based approaches are increasingly sought as alternatives to improve the efficacy or, ideally, replace the need for exogenous administration of GFs in regenerative medicine strategies. The means by which these systems evolve from biomaterials for conventional controlled release of GFs to the recent extracellular matrix (ECM)-inspired approaches for sequestering these labile molecules and regulating their spatiotemporal activity and presentation are reviewed. Focus is placed on biomaterials functionalized either with ECM components, which show promiscuous GF binding, or with targeted GF ligands (antibodies, aptamers, or peptides). The potential of synthetic platforms with abiotic affinity as cost-effective alternatives to the current biological ligands is also discussed. Overall, the various GF sequestering systems developed so far have remarkably improved the activity of GFs at reduced doses and, in some cases, completely avoided the need for their exogenous administration to guide cell fates. These bioinspired concepts thus enable the rational exploration of the full therapeutic potential of GFs in regenerative medicine.

show abstract

Enrichment of endogenous fractalkine and anti-inflammatory cells via aptamer-functionalized hydrogels

Cited by 32 publications

References 79 publications

Fetal extracellular matrix nerve wraps locally improve peripheral nerve remodeling after complete transection and direct repair in rat

Fetal extracellular matrix nerve wraps locally improve peripheral nerve remodeling after complete transection and direct repair in rat

Achieving Controlled Biomolecule–Biomaterial Conjugation

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Contact Info

Product

Resources

About