Fetal extracellular matrix nerve wraps locally improve peripheral nerve remodeling after complete transection and direct repair in rat

Ren, Tanchen; Faust, Anne E.; Merwe, Yolandi van der; Bo, Xiaochen; Johnson, Scott A.; Kandakatla, Apoorva; Gorantla, Vijay S.; Badylak, Stephen F.; Washington, Kia M.; Steketee, Michael B.

doi:10.1038/s41598-018-22628-8

Cited by 25 publications

(21 citation statements)

References 106 publications

(129 reference statements)

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“…However, these outcomes are not satisfactory and various improvements to these methods have been investigated (Eren, Öksüz, Küçükodaci, et al, 2016). Many studies have reported that tubular materials in which a cellular component is combined with a neurotropic factor and extracellular matrix (ECM) increases the regenerative potential (Chen, Yu, Ng, et al, 2018;Ren, Faust, Van Der Merwe, et al, 2018). In these reports, biodegradable synthetic tubular materials, including poly(lactide-co-glycolide) (Nune, Subramanian, Krishnan, Kaimal, & Sethuraman, 2017) or chitosan tubes (Meyer, Wrobel, Raimondo, et al, 2016) have been used.…”

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confidence: 99%

A scaffold‐free Bio 3D nerve conduit for repair of a 10‐mm peripheral nerve defect in the rats

et al. 2019

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Introduction A Bio 3D printed nerve conduit was reported to promote nerve regeneration in a 5 mm nerve gap model. The purpose of this study was to fabricate Bio 3D nerve conduits suitable for a 10 mm nerve gap and to evaluate their capacity for nerve regeneration in a rat sciatic nerve defect model. Materials and Methods Eighteen F344 rats with immune deficiency (9–10 weeks old; weight, 200–250 g) were divided into three groups: a Bio 3D nerve conduit group (Bio 3D, n = 6), a nerve graft group (NG, n = 6), and a silicon tube group (ST, n = 6). A 12‐mm Bio 3D nerve conduit or silicon tube was transplanted into the 10‐mm defect of the right sciatic nerve. In the nerve graft group, reverse autografting was performed with an excised 10‐mm nerve segment. Assessments were performed at 8 weeks after the surgery. Results In the region distal to the suture site, the number of myelinated axons in the Bio 3D group were significantly larger compared with the silicon group (2,548 vs. 950, p < .05). The myelinated axon diameter (MAD) and the myelin thickness (MT) of the regenerated axons in the Bio 3D group were significantly larger compared with those of the ST group (MAD: 3.09 vs. 2.36 μm; p < .01; MT: 0.59 vs. 0.40 μm, p < .01). Conclusions This study indicates that a Bio 3D nerve conduit can enhance peripheral nerve regeneration even in a 10 mm nerve defect model.

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confidence: 99%

A scaffold‐free Bio 3D nerve conduit for repair of a 10‐mm peripheral nerve defect in the rats

et al. 2019

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“…These results were in agreement with (27) were evaluated the use of the decellularized human umbilical artery (hUA) as nerve guidance conduit, the authors showed that decellularized hUAs after implantation were rich in nerve fibers and characterized by improved sciatic functional index (SFI) values and supported elongation and bridging of the 10 mm nerve gap in rats. The study by (28) implantation of a fetal porcine urinary bladder extracellular matrix (fUB-ECM) in a trigeminal, infra orbital nerve branch transection and direct endto-end repair model in rat, significantly improved epi-and endoneurial organization and increased both neovascularization and growth associated protein-43 (GAP-43) expression at PN repair sites. Scaffolds seeded with HUC-MSCs of which the environment had been conditioned in such a way to obtain the factors supporting nerve regeneration.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Acellular-Lyophilized Human Umbilical Cord Powder Seeded with Human Umbilical Cord Mesenchymal Stem Cells in Peripheral Nerve Regeneration in Dogs Models

Ali A.N. AL-Zaidi,

Zeyad A. Shabeeb,

Hameed A.K. Al-Timmemi

2021

MLU

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Treatment of injuries to peripheral nerves after a segmental defect is one of the most challenging surgical problems. Therapies for peripheral nerves injury are largely ineffective. Therefore, the current study was conducted to investigate effects either acellular human umbilical cord tissue powder on repair 1 cm critical size defect radial nerve guided by bovine urinary bladder matrix conduit alone or seeded with mesenchymal stem cells derived from human umbilical cord. For this purpose, sixteen healthy mongrel dogs were used. They were randomly divided into two equal groups (n=8), before proceeding with the surgical procedures, the dogs were generally anesthetized and aseptic conditions were established. Then, the surgery was carried out with the aid of magnifying lens. All dogs subjected to radial nerve neurotmesis from the right limb. In the scaffold group, a 1cm of radial nerve was transected and resulted gap bridged with a portion of 14mm acellular bovine urinary bladder submucosa conduit which was then filled with 0.05mg acellular human umbilical cord powder and then sutured to the two stumps using 6-0 nylon epineural by simple interrupted pattern. In combination group, the gap was bridged with acellular bovine urinary bladder submucosa conduit which was filled with 0.05mg acellular human umbilical cord powder then seeded with 50 µl (5x10 6) mesenchymal stem cells derived from human umbilical cord, then fixed onto two stumps using 6-0 nylon epineural by simple interrupted pattern. Each group was further divided into two equal subgroups (n=4) categorized according to the post operation periods, that were 8 and 16 th weeks for neurohistopathological examinations. The histopathological examination of regenerated nerve sections (two samples of 5 mm length each) were collected from the middle (coaptation site) and distal segments were used to determine the degree of the radial nerve regeneration. The histopathological findings relative convergence between the scaffold and combination groups in their components such as the increased number of Schwann cells proliferation, parallel arrangement of nerve fibers and remarkable angiogenesis on 16 th weeks. In conclusion; this study showed that the decellularized both scaffolds alone or seeded with mesenchymal stem cells derived from human umbilical cord could support the radial nerve regeneration and allow the reinnervation of the target organ.

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“…For direct end-to-end suturing of the injured nerve, a nerve wrap for anastomosis site is necessary to prevent connective tissue invasion into the anastomosis site and promote nerve regeneration. After membrane tissue is decellularized, such as porcine SIS-dECM and fetal urinary bladder dECM (fUB-dECM), it is used directly to wrap the nerve anastomosis site [ 112 ]. However, dECM-based wrap derived from non-membraneous tissue, such as sciatic nerve, undergoes a series of molding process as follows: the nerve derived-dECM is transformed into a powder, the resulting powder is solubilized in 0.3% acetic acid, the digested solution is transferred to a plate, and the solution-to-membrane transition of dECM is induced by drying at room temperature [ 40 ].…”

Section: Applications Of Xenogeneic Decm-based Biomaterialsmentioning

confidence: 99%

Xenogeneic Decellularized Extracellular Matrix-based Biomaterials For Peripheral Nerve Repair and Regeneration

Javed

2021

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: Peripheral nerve injury could lead to either impairment or a complete loss of function for affected patients, and a variety of nerve repair materials have been developed for surgical approaches to repair it. Although autologous or allologous tissue-derived biomaterials remain preferred treatment for peripheral nerve injury, the lack of donor sources has led biomedical researchers to explore more other biomaterials. As a reliable alternative, xenogeneic decellularized extracellular matrix (dECM)-based biomaterials have been widely employed for surgical nerve repair. The dECM derived from animal donors is an attractive and unlimited source for xenotransplantation. Meanwhile, as an increasingly popular technique, decellularization could retain a variety of bioactive components in native ECM, such as polysaccharides, proteins, and growth factors. The resulting dECM-based biomaterials preserve a tissue's native microenvironment, promote Schwann cells proliferation and differentiation, and provide cues for nerve regeneration. Although the potential of dECM-based biomaterials as a therapeutic agent is rising, there are many limitations of this material restricting its use. Herein, this review discusses the decellularization techniques that have been applied to create dECM-based biomaterials, the main components of nerve ECM, and the recent progress in the utilization of xenogeneic dECM-based biomaterials through applications as a hydrogel, wrap, and guidance conduit in nerve tissue engineering. In the end, the existing bottlenecks of xenogeneic dECM-based biomaterials and developing technologies that could be eliminated to be helpful for utilization in the future have been elaborated.

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Fetal extracellular matrix nerve wraps locally improve peripheral nerve remodeling after complete transection and direct repair in rat

Cited by 25 publications

References 106 publications

A scaffold‐free Bio 3D nerve conduit for repair of a 10‐mm peripheral nerve defect in the rats

A scaffold‐free Bio 3D nerve conduit for repair of a 10‐mm peripheral nerve defect in the rats

Efficacy of Acellular-Lyophilized Human Umbilical Cord Powder Seeded with Human Umbilical Cord Mesenchymal Stem Cells in Peripheral Nerve Regeneration in Dogs Models

Xenogeneic Decellularized Extracellular Matrix-based Biomaterials For Peripheral Nerve Repair and Regeneration

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