Enrichment and characteristics of ammonia-oxidizing archaea in wastewater treatment process

Abstract

This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Abstract: High purity ammonia-oxidizing archaea (AOA) culture containing a single AOA strain was enriched from the filtering materials of biological aerated filter. The concentration of AOA reached 3.27×10 7 copies/mL, while its proportion was 91.40%. The AOA amoA gene sequence belonged to Nitrososphaera cluster. Ammonia concentration significantly influenced the growth of AOA in culture, while total organic carbon (TOC) concentration had no obvious effect. The optimum ammonia concentration, temperature, pH and DO concentration for growth of AOA were 1 mM, 30 ˚C, 7.5 and 2.65 mg/L, respectively. Under the optimum growth conditions, the AOA abundance and ammonia oxidation rate were 3.53×10 7 copies/mL and 2.54×10 -10 mg/(copies·d).

Ammonia-Oxidizing Archaea (AOA) Play with Ammonia-Oxidizing Bacteria (AOB) in Nitrogen Removal from Wastewater

“…Roy et al [ 35 ] found that AOA outnumber AOB and contribute to ammonia oxidation in the biofilm samples of trickling filter and moving bed bioreactor treating municipal wastewater, with the abundance of the archaeal amoA gene 2-3 orders of magnitude higher than that of the bacterial amoA gene. Chen et al [ 36 ] also had the same observation in the biofilm in biological aerated filters for municipal wastewater treatment, and a single AOA strain was enriched from the filtering materials using synthetic medium [ 37 ].…”

Response of ammonia‐oxidizing archaea and bacteria to streptomycin sulfate and penicillin in coastal wetlands along the Bohai Rim

“…There are great differences in biochemical composition and metabolic mechanisms between bacteria and archaea. Antibiotics are thought to completely inhibit the activity and growth of AOB and have little effect on AOA (Chen et al, 2017; Lehtovirta‐Morley et al, 2016; Martens‐Habbena, Berube, Urakawa, de la Torre, & Stahl, 2009). Laboratory studies on some specific strains have also indicated that AOA are insensitive to antibiotics (Lehtovirta‐Morley et al, 2016).…”

Effect of sulfadiazine and trimethoprim on activated sludge performance and microbial community dynamics in laboratory‐scale membrane bioreactors and sequencing batch reactors at 8°C

“…Keeping in mind high amount of nitrates in MBR effluents it could be assumed that nitrogen removing bacteria with developed antibiotic resistance outcompeted archaea because of faster growth rate and not optimal for archaea environment. Previously, Chen et al showed AOA inhibition in DO concentrations higher than 4 mg L −1 . Similarly, Euryarchaeota growth could be suppressed by high DO concentration and active bacterial competitors on day 60 of Period II.…”