Enqu�te sur la fr�quence des infections virales au cours des maladies respiratoires aigu�s de l'enfant

Gernez-Rieux, C; Breton, A; Samaille, J; Voisin, C; Lelong, M

doi:10.1007/bf01243857

Cited by 3 publications

(2 citation statements)

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“…Ion-exchange chromatography. Fractionation on DEAE-cellulose was carried out as described by Gernez-Rieux et al (1963), either before or after gel chromatography on 1% agarose. A column (2.5cm x 5.5cm) was packed with a slurry of DEAEcellulose suspended in 5mm-potassium phosphate buffer, pH8.0.…”

Section: Methodsmentioning

confidence: 99%

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Biosynthesis of respiratory-tract mucins. Incorporation of radioactive precursors into glycoproteins by canine tracheal explants in vitro

Ellis¹,

Stahl²

1973

Biochemical Journal

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1. Canine tracheal explants, cultured in medium 199, actively incorporated radioactive precursors into secreted macromolecules in vitro. 2. Puromycin, 6-diazo-5-oxo-l-norleucine and ouabain markedly inhibited the incorporation of these precursors. 3. Exogenous glucosamine at concentrations above 20mm caused a greater than 50% inhibition of the incorporation of l-[G-(3)H]fucose and l-[U-(14)C]serine. 4. Carbohydrate content of the purified secretions was approximately 50% and consisted principally of galactose, N-acetylglucosamine, N-acetylgalactosamine, fucose and sialic acids. 5. Chromatography on DEAE-cellulose and Bio-Gel A-150m and equilibrium density-gradient centrifugation in a CsCl gradient confirmed the presence of mucous glycoproteins. 6. Electrophoresis on 1% agarose gels gave profiles that were identical with canine respiratory mucus obtained in vivo. 7. These results support the utility of the explant system for studies of respiratory secretions.

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Section: Methodsmentioning

confidence: 99%

“…The two Bio-Gel fractions were concentrated, dialysed and then further chromatographed on DEAE-cellulose as described for respiratory expectorations by Gernez-Rieux et al (1963). Elution from these columns was with 3 bed volumes of each buffer as described under 'Methods'.…”

Section: Fractionation and Characterization Of The Secreted Macromolementioning

confidence: 99%

Biosynthesis of respiratory-tract mucins. Incorporation of radioactive precursors into glycoproteins by canine tracheal explants in vitro

Ellis¹,

Stahl²

1973

Biochemical Journal

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Vorkommen hämagglutinationshemmender Antikörper gegen Parainfluenzaviren bei verschiedenen Tierspecies

Sandow

Schmidt

1967

Z. med. Mikrobiol. u. Immunol.

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Untersuchungen über das Vorkommen hämagglutinationshemmender Antikörper gegen Parainfluenzaviren in der Bevölkerung

Schmidt

Tauchnitz

Kühn

1964

Zeitschr. f. Hygiene.

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Enqu�te sur la fr�quence des infections virales au cours des maladies respiratoires aigu�s de l'enfant

Cited by 3 publications

References 0 publications

Biosynthesis of respiratory-tract mucins. Incorporation of radioactive precursors into glycoproteins by canine tracheal explants in vitro

Biosynthesis of respiratory-tract mucins. Incorporation of radioactive precursors into glycoproteins by canine tracheal explants in vitro

Vorkommen hämagglutinationshemmender Antikörper gegen Parainfluenzaviren bei verschiedenen Tierspecies

Untersuchungen über das Vorkommen hämagglutinationshemmender Antikörper gegen Parainfluenzaviren in der Bevölkerung

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