Enolase isoenzymes as markers of differentiation in teratocarcinoma cells and normal tissues of mouse

Fletcher, Lynne; Rider, Christopher C.; Taylor, C. B.; Adamson, Eileen D.; Luke, Barbara M.; Graham, Chris

doi:10.1016/0012-1606(78)90041-6

Cited by 70 publications

(21 citation statements)

References 22 publications

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“…The finding of the y subunit of enolase, a well-de scribed nerve cell marker [Fletcher et al, 1978;Schmechel et al, 1980], further sub stantiates this conclusion although other markers of nerve cells, the neurofilament subunit polypeptides, are not detectable at any stage of in vitro differentiation. A similar finding has been published by Velasco et al [ 1985], showing the expression of the neuronspecific enolase without concommitant ex pression of the neurofilament subunit poly peptides in undifferentiated cells of human medulloblastomas, which are primitive neu roectodermal tumors [Velasco et al, 1985].…”

Section: Discussionmentioning

confidence: 87%

“…The aa homodimer is characteristic for the nonneural, basically glial cells, while the pres ence of the y subunit in either homodimer or heterodimer form indicates nerve cell devel opment, as this subunit is formed exclusively in nerve or neuroendocrine cells [Schmechel et al, 1980]. During ontogenesis the em bryonic brai n was shown to express the a sub unit only, and y subunit synthesis was first seen together with the appearance of the first postmitotic neurons [Fletcher et al, 1978;Schmechel et al, 1980]. The enolase pattern of the neuro 2a clonal cell line exemplifies this shift in vitro, since with the embryonic aa homodimer a signifi cant proportion of ay heterodimer is detecta ble.…”

Section: Enolase Isozyme Pattern O F the 95-bc Cell Linementioning

confidence: 99%

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Cytoskeletal Characterization of a Neuroectodermal Cell Line and Embryonic Mouse Brain

Zákány¹,

Murach²,

Fundele³

et al. 1987

Dev Neurosci

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The cytoskeleton of a newly isolated mouse embryo brain-derived cell line and of dissected mesencephalon-rhombencephalon samples of the 10- to 11-day-old mouse embryo, constituted of a ventricular zone only, has been examined by immunocytochemistry, electrophoretic separation and Western blotting techniques. In accordance with their epithelial organization, the ventricular cells contain cytokeratins as main constituents of their cytoskeleton. Vimentin has been also revealed as early as day 10.5 of gestation. The complex cytokeratin polypeptide pattern puts this histological type of epithelia into the category of complex, rather than simple, epithelia and calls for explanations other than keratinization for the presence and functional role of the high-molecular weight, basic keratins. The cytoskeletal composition of the embryo brain-derived gliogenic cell line reflects the vimentin- and cytokeratin-positive character of the ventricular cells.

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Section: Discussionmentioning

confidence: 87%

Section: Enolase Isozyme Pattern O F the 95-bc Cell Linementioning

confidence: 99%

Cytoskeletal Characterization of a Neuroectodermal Cell Line and Embryonic Mouse Brain

Zákány¹,

Murach²,

Fundele³

et al. 1987

Dev Neurosci

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“…Glial cells with intermediate filaments can also be identified [Jones-Villeneuve et al, 1982]. In addition, some murine EC cells produce the a, p and y subunits of enolase with a-enolase appearing earlier than the other subunits [Fletcher et al, 1978]. Final ly, the EC cells of experimental teratomas in mice may transform to neuroblasts and later proliferate, migrate and differentiate to other nervous cells [Tresman and Evans, 1975].…”

Section: Embryonal Carcinoma In Animalsmentioning

confidence: 99%

Teratomas and Allied Germ Cell Tumors: Common Origin and Diverging Paths of Differentiation

Pesce

Carli²

1988

Path Immunopathol Res

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“…The expression of these forms is developmentally determined. For example, in rat fetal muscle, the aa-form is dominant and as the rat matures, the ßß form becomes dominant [19], The exact function of the different forms and their tissue specificities are unclear. A recent study shows that they may play a role in the intracellu lar compartmentation of metabolism [20].…”

Section: Introductionmentioning

confidence: 99%

Induced Expression of Alpha-Enolase in Differentiated Diffuse Large Cell Lymphoma

My²,

Al‐Katib³

1994

Enzyme Protein

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A unique protein has been detected that is associated with the differentiation of diffuse large cell lymphoma (DLCL). The WSU-DLCL human cell line was cultured in the absence or presence of the biological agent, Bryostatin 1 (Bryo 1 ). Cellular proteins of parent and differentiated WSU-DLCL cells were analyzed using one- and two-dimensional polyacrylamide gel electrophoresis (ID and 2D PAGE). In the ID PAGE, a unique protein band of molecular mass ~ 47 kD was detected in the differentiated, but not the parent cells. Amino acid sequence of the band indicated the presence of more than one protein. The 2D PAGE analysis showed that one of the proteins of interest had an isoelectric point of 7.4. Partial amino acid sequencing of the spot by tryptic digest showed 100% homology with a-enolase. a-Enolase is a nonneuronal enzyme involved in the glycolytic pathway. This is the first report on the induction of a-enolase in human DLCL after treatment with the natural biological agent, Bryol. We suggest that aenolase may play a significant role in the differentiation of lymphoma in man.

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Enolase isoenzymes as markers of differentiation in teratocarcinoma cells and normal tissues of mouse

Cited by 70 publications

References 22 publications

Cytoskeletal Characterization of a Neuroectodermal Cell Line and Embryonic Mouse Brain

Cytoskeletal Characterization of a Neuroectodermal Cell Line and Embryonic Mouse Brain

Teratomas and Allied Germ Cell Tumors: Common Origin and Diverging Paths of Differentiation

Induced Expression of Alpha-Enolase in Differentiated Diffuse Large Cell Lymphoma

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