Cytoskeletal Characterization of a Neuroectodermal Cell Line and Embryonic Mouse Brain

Zákány, József; Murach, Karl-Friedrich; Fundele, Reinald; Illmensee, Karl

doi:10.1159/000111612

Dev Neurosci

1987

DOI: 10.1159/000111612

|View full text |Cite

Cytoskeletal Characterization of a Neuroectodermal Cell Line and Embryonic Mouse Brain

József Zákány¹,

Karl-Friedrich Murach²,

Reinald Fundele³

et al.

Abstract: The cytoskeleton of a newly isolated mouse embryo brain-derived cell line and of dissected mesencephalon-rhombencephalon samples of the 10- to 11-day-old mouse embryo, constituted of a ventricular zone only, has been examined by immunocytochemistry, electrophoretic separation and Western blotting techniques. In accordance with their epithelial organization, the ventricular cells contain cytokeratins as main constituents of their cytoskeleton. Vimentin has been also revealed as early as day 10.5 of gestation. T… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1989

1992

Publication Types

Select...

Article3

Relationship

Self Cite2

Independent1

Authors

Journals

Cited by 3 publications

References 27 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Protein synthesis in murine organs during postimplantation development detected by two‐dimensional gel electrophoresis

et al. 1992

Self Cite

View full text Add to dashboard Cite

Mouse embryos were isolated from the uterus on days 10 to 11 of gestation and incubated in Dulbecco's modified Eagle's medium (DMEM) with [35S]methionine for 4 h. Subsequently, their hearts and the brains were dissected. The brain was divided into three parts, containing the telencephalon, mesencephalon, and myelencephalon. These tissues were then processed for two-dimensional (2-D) gel electrophoresis. Protein synthesis of the isolated tissues was analyzed for organ-and cell lineage-specific patterns. We studied proteins with isoelectric points (pI) ranging from 4 to 10 and relative molecular weights (M(r)) varying from 10000 to 200000 and found several significant quantitative and qualitative differences between the tissues and the developmental stages analyzed. In particular, we were able to distinguish between protein spots that we now attribute putatively to the corresponding embryonic organs. These differences may reflect some of the organ- and cell lineage-specific changes in protein synthesis and gene expression during early mammalian differentiation.

show abstract

Protein synthesis in murine organs during postimplantation development detected by two‐dimensional gel electrophoresis

et al. 1992

Self Cite

View full text Add to dashboard Cite

show abstract

Protein synthesis in embryonic tissues during mouse postimplantation development

Murach

Frei

Gerhäser

et al. 1990

J of Cellular Biochemistry

Self Cite

View full text Add to dashboard Cite

Mouse embryos of the NMRI strain between the 7th and 9th day of gestation were isolated from the uterus and dissected into the various tissue derivatives in order to investigate newly synthesized proteins during morphogenesis. The day 7 embryo was fragmented into trophoblast and ectoplacental cone, distal and proximal endoderm, extraembryonic and embryonic ectoderm. The day 8 and day 9 embryos were divided into trophoblast and placental anlage, yolk sac, amnion, and allantois, as well as cranial, central, and caudal embryonic tissue. The intact embryos were incubated in Dulbecco's minimum essential medium in the presence of 35S-methionine for 4 h, then dissected into the various fragments, and further processed for two-dimensional gel electrophoresis. Protein synthesis of the isolated tissue derivatives was analyzed and compared for the three developmental stages. Concerning the proteins with isoelectric points in the range of 4.5 to 8.0 and molecular weight ratio (M(r)) values between 20,000 and 200,000, we found several significant quantitative and qualitative differences in the various tissue fragments. In addition, we observed further quantitative and qualitative differences in protein synthesis during the postimplantation period investigated. We propose that the differences reflect some of the cell lineage- and developmental stage-specific changes in gene expression during early mammalian differentiation.

show abstract

Regulation of the Cellular Retinoid-Binding Proteins and Their Messenger Ribonucleic Acids during P19 Embryonal Carcinoma Cell Differentiation Induced by Retinoic Acid

Blaner

Goodman

et al. 1989

Molecular Endocrinology

View full text Add to dashboard Cite

P19 embryonal carcinoma (EC) cells can be induced to differentiate in vitro into a variety of cell types by treatment with different concentrations of retinoic acid (RA). A study was conducted to explore the regulation of expression of the genes for cellular retinoic acid-binding protein (CRABP) and cellular retinol-binding protein (CRBP) in P19 cells induced to differentiate by RA. For each retinoid-binding protein, both the level of specific mRNA and of immunoreactive protein were measured, respectively, by RNase protection assay and by a specific RIA. Dramatic increases in CRABP and CRBP were seen, at both the mRNA and protein levels, during the RA-induced differentiation. CRBP induction differed from that of CRABP in several major ways. 1) Induction of CRBP occurred at lower concentrations of RA (10(-9) M) than did that of CRABP (10(-8)-10(-7) M). 2) CRBP induction was an early response (within 3 h) to RA treatment, whereas CRABP induction occurred at a later time (12-24 h). 3) Induction of CRABP mRNA by RA was blocked by the protein synthesis inhibitor cycloheximide, whereas induction of CRBP mRNA was not. 4) Several differentiation inducers were tested for their effects on the expression of CRABP and CRBP in P19 cells. CRBP induction occurred with a wider spectrum of inducers than did that of CRABP. 5) In addition, the induction of CRABP and CRBP mRNAs by RA was examined in six different cell lines, including three EC lines. CRBP induction occurred in a wider spectrum of cell lines than did that of CRABP. The induction of CRABP in EC cells seems, in general, to correlate with their differentiation into neuron-like cells. Taken together, our results suggest that CRBP induction may be a direct response to RA and represent a general event in RA-induced cell differentiation, whereas CRABP induction may be an indirect response and represent a later event restricted to only certain differentiation pathways. CRBP may be an early response gene induced by RA.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Cytoskeletal Characterization of a Neuroectodermal Cell Line and Embryonic Mouse Brain

Cited by 3 publications

References 27 publications

Protein synthesis in murine organs during postimplantation development detected by two‐dimensional gel electrophoresis

Protein synthesis in murine organs during postimplantation development detected by two‐dimensional gel electrophoresis

Protein synthesis in embryonic tissues during mouse postimplantation development

Regulation of the Cellular Retinoid-Binding Proteins and Their Messenger Ribonucleic Acids during P19 Embryonal Carcinoma Cell Differentiation Induced by Retinoic Acid

Contact Info

Product

Resources

About