ENO1 promotes tumor proliferation and cell adhesion mediated drug resistance (CAM-DR) in Non-Hodgkin's Lymphomas

Zhu, Xinghua; Miao, Xiaobing; Wu, Yong; Li, Chunsun; Guo, Yan; Liu, Yushan; Chen, Yali; Lu, Xiaoyun; Wang, Yuchan; He, Song

doi:10.1016/j.yexcr.2015.05.020

Cited by 51 publications

(56 citation statements)

References 21 publications

(22 reference statements)

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“…The procedures were carried out similarly to previously described methods [14]. A mouse monoclonal anti-TRIP6 antibody (1:100, Santa Cruz Biotechnology, USA) and an anti-Ki-67 antibody (1:100, Santa Cruz Biotechnology, USA) were used for primary antibody incubation at 4°C overnight.…”

Section: Immunohistochemistry and Antibodiesmentioning

confidence: 99%

“…Staining of TRIP6 and Ki-67 was reviewed and scored using a semi-quantitative immunoreactivity score (IRS) as described previously [14]. Briefly, the intensity of immunostaining was 0-3 (0, negative; 1, weak; 2, moderate; 3, strong).…”

Section: Assessment Of Immunohistochemistrymentioning

confidence: 99%

“…Western blot was carried out as previously described [14]. The antibodies used in this study included the following: anti- , and anti-Myc tag antibody (1:1000, Abcam, UK).…”

Section: Western Blot and Cell Fractionationmentioning

confidence: 99%

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Overexpression of TRIP6 promotes tumor proliferation and reverses cell adhesion-mediated drug resistance (CAM-DR) via regulating nuclear p27Kip1 expression in non-Hodgkin’s lymphoma

Miao

et al. 2015

Tumor Biol.

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Recent studies have identified that thyroid hormone receptor-interacting protein 6 (TRIP6) is implicated in tumorigenesis. However, the functional role of TRIP6 in non-Hodgkin's lymphoma (NHL) has never been elucidated. In this study, we demonstrated that TRIP6 is reversely correlated with the clinical outcomes of NHL patients. Western blot and immunohistochemical analysis revealed that TRIP6 expression is lower in indolent lymphoma than in progressive lymphoma. Kaplan-Meier survival curves indicated that the upregulation of TRIP6 is significantly associated with poor overall survival. Moreover, patients with higher expression of TRIP6 are prone to shorter time to recurrence. Furthermore, we also found that TRIP6 can promote the proliferation of NHL cells via regulating cell cycle progression. In addition, adhesion of lymphoma cells to fibronectin (FN) decreased TRIP6 expression, which led to the upregulation of nuclear p27(Kip1) expression by decreasing phosphorylation of p27(Kip1) at T157. Importantly, overexpression of TRIP6 can reverse cell adhesion-mediated drug resistance (CAM-DR) phenotype in NHL. In summary, these results suggest that TRIP6 is a novel prognostic indicator for NHL patients and may shed new insights into the important role of TRIP6 in cancer development.

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Section: Immunohistochemistry and Antibodiesmentioning

confidence: 99%

Section: Assessment Of Immunohistochemistrymentioning

confidence: 99%

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Overexpression of TRIP6 promotes tumor proliferation and reverses cell adhesion-mediated drug resistance (CAM-DR) via regulating nuclear p27Kip1 expression in non-Hodgkin’s lymphoma

Miao

et al. 2015

Tumor Biol.

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“…ENO1 (enolase 1, alpha-enolase) was quantified readily in samples processed only with FPD methods, but not with chemical precipitation (Figure 5A). ENO1 is a glycolytic enzyme that is important in cancer research as it has been shown to bind to the c-myc promoter and has tumor suppressor function (21). In contrast, EPDR1 (ependymin related 1 transmembrane protein), a cell adhesion protein, was quantified mainly in samples precipitated with TCA (Figure 5B) (22).…”

Section: Resultsmentioning

confidence: 99%

Filter-Based Protein Digestion (FPD): A Detergent-Free and Scaffold-Based Strategy for TMT Workflows

2018

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High-throughput proteome profiling requires thorough optimization to achieve comprehensive analysis. We developed a filter aided sample preparation (FASP)-like, detergent-free method - termed Filter-based Protein Digestion (FPD). We compared FPD to protein extraction methods commonly used in isobaric tag-based proteome profiling, namely trichloroacetic acid (TCA) and chloroform-methanol (C-M) precipitation. We divided a mammalian whole cell lysate from the SH-SY5Y neuroblastoma cell line for parallel protein processing with TCA (n=3), C-M (n=2), and FPD using either 10 kDa (n=3) or 30 kDa (n=3) molecular weight cut-off membranes. We labeled each sample with tandem mass tag (TMT) reagents to construct a TMT11-plex experiment. In total, 8,654 proteins were quantified across all samples. Pairwise comparisons showed very little deviation for individual protein abundance measurements between the two FPD methods, while TCA and FPD showed the most difference. Specifically, membrane proteins were more readily quantified when samples were processed using TCA precipitation than other methods tested. However, globally, only 4% of proteins differed greater than 4-fold in the most divergent pair of protein extraction methods (i.e., FPD10 and TCA). We conclude that the detergent-free FPD strategy, particularly using the faster-flowing 30kDa filter, is a seamless alteration to high-throughput TMT workflows.

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“…Its overexpression has been associated with multiple tumors. [62][63][64] In many of these tumors, ENO1 promoted tumorgenesis via activating and regulating the PI3K/ AKT signaling pathway. 63 It is shown that the knockdown of ENO1 expression led to suppressed cell growth and migration by inactivating the PI3K/Akt pathway in glioma cells.…”

Section: Discussionmentioning

confidence: 99%

Diabetic Retinopathy and Laser Therapy in Rats: A Protein-Protein Interaction Network Analysis

et al. 2017

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Introduction: Diabetic retinopathy (DR) is a serious microvascular complication of diabetes which can cause vision loss or blindness ultimately. Non enzymatic glycation of proteins leads to advanced glycation end products (AGEs) in DR. Since laser therapy is a well-established method, in this study, protein-protein interaction (PPI) network is applied for protein targets in DR disease in rats treated by laser. Methods: In this study, we focused on articles that investigated and compared the proteome profiles of DR rats with healthy control and also DR rats before and after laser therapy. The networks of related differentially expressed proteins were explored using Cytoscape version 3.3.0, the PPI analysis methods and ClueGO. Results: Analysis of PPI network of 37 related proteins to DR rats including 108 nodes, introduced 10 hub-bottleneck proteins and 5 concerned biochemical pathways. On the other hand, PPI analysis of related proteins to DR rats before and after laser therapy corresponded to 33 proteins and 2 biological pathways. Discussion: Centrality and cluster screening identified hub-bottelneck genes, including Aldoa, HSPD1, Pgam2, Mapk3, SLC2A4, Ctnnb1, Ywhab, HSPA8, GAPDH and Actb for DR rats versus healthy control and ENO1, Aldoa, GAPDH for DR samples after laser therapy. Conclusion: Gene expression analysis of the DR samples treated via laser therapy provides a molecular evidence in support of the therapeutic effect of laser.

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ENO1 promotes tumor proliferation and cell adhesion mediated drug resistance (CAM-DR) in Non-Hodgkin's Lymphomas

Cited by 51 publications

References 21 publications

Overexpression of TRIP6 promotes tumor proliferation and reverses cell adhesion-mediated drug resistance (CAM-DR) via regulating nuclear p27Kip1 expression in non-Hodgkin’s lymphoma

Overexpression of TRIP6 promotes tumor proliferation and reverses cell adhesion-mediated drug resistance (CAM-DR) via regulating nuclear p27Kip1 expression in non-Hodgkin’s lymphoma

Filter-Based Protein Digestion (FPD): A Detergent-Free and Scaffold-Based Strategy for TMT Workflows

Diabetic Retinopathy and Laser Therapy in Rats: A Protein-Protein Interaction Network Analysis

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