Enlargement of the Genetic Diversity for Grain Quality in Bread Wheat Through Alien Introgression

Abstract

Diverse technological purposes of bread wheat grain requires a broadening the genetic base of selection for quality traits. Introgressions potentially may affect technological properties of grain and fl our and add to a genetic variability of the trait. The aim of this work was to investigate the infl uence of introgressions from exotic tetraploid wheat Triticum timopheevii and wild cereal species, Aegilops speltoides and Aegilops markgrafi i into bread wheat on grain quality. The introgression from T. timopheevii including the microsatellite marker Xgwm636 into 2AS chromosome of cv. Saratovskaya 29 resulted in a signifi cant increase of gluten content in grain. The effect was confi rmed by re-introducing the recombinant chromosome again into the same genetic background. The analogous effect was observed in the line of winter cultivar Alcedo with the similar alien fragment in 2AS chromosome inherited from Ae. markgrafi i . Introgression of 5S chromosome of Aegilops speltoides carrying the gene Ha-Sp for grain softness into hard-grain bread wheat cultivars resulted in obtaining the genotypes with soft grain texture. Combining two dominant genes for grain softness Ha and Ha-Sp in one genotype allowed us to obtain the plants with grain having the new milling properties -very low vitreousness (about 30 %) and small particle size (about 10 μm).

Prospects of molecular genetic approaches in controlling technological properties of wheat grain in the context of the "grain – flour – bread" chain

“…Вместе с ним и другие гены близкородственных видов можно использовать для увеличения содержания белка в зерне мягкой пшеницы (De Pace et al, 2001;Zanetti et al, 2001;Kunert et al, 2007) и для улучшения хлебопекарных свойств (Garg et al, 2014). Источниками высокого содержания клейковины в зерне могут быть виды T. timophee vii, Aegilops speltoides (Pshenichnikova et al, 2015), а также Ae. markgrafii.…”

Properties of grain, flour and dough in bread wheat lines with Aegilops markgrafii introgressions

“…This parameter well correlates with wet gluten content (Kozmina 1969;Kulkarni et al 1987;Zhang et al 2008). Recently, it has been shown that the introgression from Triticum timopheevii in the same region of the short arm of 2A chromosome significantly improves this trait (Pshenichnikova et al 2015). The introgressed segment from Ae.…”