Enhancing the osteogenic differentiation of aligned electrospun poly(L-lactic acid) nanofiber scaffolds by incorporation of bioactive calcium silicate nanowires

Fu, Zeyu; Li, Dejian; Lin, Kaili; Zhao, Bin; Wang, Xudong

doi:10.1016/j.ijbiomac.2022.11.224

Cited by 16 publications

(12 citation statements)

References 48 publications

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“…For instance, by rotating the collector, nanofibers can be stretched parallel to each other. Fu et al [116] adjusted the rotational speed of the electrospinning collector to prepare oriented PLLA scaffolds (Figure 2A). Their investigation revealed that low rotation speeds did not promote overall nanofiber orientation, but high rotation speeds resulted in an overall fiber orientation within the scaffold (Figure 2B).…”

Section: Electrospinningmentioning

confidence: 99%

“…[124][125][126][127] This technique involves the diffusion of supercritical fluid into the polymer matrix at specific temperature and pressure conditions, resulting in a homogeneous system. Subsequent pressure drop or temperature process is when the supercritical fluid in the homogeneous [116] Copyright 2023, Elsevier C) Chemically modified nanofiber mats subjected to mold-constrained expansion to achieve porous structures. D) SEM image of a constrained-expandable nanofibrous scaffold.…”

Section: Supercritical Fluid Foamingmentioning

confidence: 99%

“…B) SEM images comparing randomly and aligned nanofibers at varying rotation speeds. Reproduced with permission [116]. Copyright 2023, Elsevier C) Chemically modified nanofiber mats subjected to mold-constrained expansion to achieve porous structures.…”

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confidence: 99%

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Oriented Porous Polymer Scaffolds in Tissue Engineering: A Comprehensive Review of Preparation Strategies and Applications

Liu,

Wang,

Kuang

2023

Macro Materials & Eng

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The pursuit of effective therapeutic strategies for tissue damage has prompted extensive scholarly investigations worldwide. Tissue engineering has emerged as a prominent approach, particularly through the utilization of artificial scaffolds that closely resemble the natural extracellular matrix (ECM). These scaffolds exhibit multi‐scale topological structures and surface physicochemical properties, which significantly influence cellular behavior, thereby attracting considerable attention from numerous researchers. This comprehensive review is concentrated on the primary techniques employed in the fabrication of biodegradable polymer scaffolds possessing oriented porous structures, and the most recent advancements in tissue engineering research are presented. Significantly, the profound influence of scaffold surface characteristics is underscored on cellular behavior, elucidating the superiority of oriented pore structures over disordered ones in mimicking the distinctive attributes of the ECM. Enhanced cell adhesion, proliferation, and tissue differentiation represent notable advantages associated with oriented porous scaffolds. Additionally, the critical interplay between scaffold structure, performance, and functionalization is emphasized, highlighting the imperative to optimize the clinical application of tissue engineering scaffolds.

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Section: Electrospinningmentioning

confidence: 99%

Section: Supercritical Fluid Foamingmentioning

confidence: 99%

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Oriented Porous Polymer Scaffolds in Tissue Engineering: A Comprehensive Review of Preparation Strategies and Applications

Liu,

Wang,

Kuang

2023

Macro Materials & Eng

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“…[ 1,2 ] Inducing the rapid differentiation and maturation of stem cells is a fundamental goal in tissue engineering and stem cell therapy. [ 3–5 ] There is increasing evidence that biomaterial surface topography is an essential factor that selectively influences cell adhesion and, in some cases, promotes cell differentiation. [ 6 ] To date, various biomaterials with different topographies at both the micrometer and nanometer scales, such as columns, grooves, and submicron fibers, have been applied to study the influence of topography on cellular functions.…”

Section: Introductionmentioning

confidence: 99%

PIP2 Alteration Caused by Elastic Modulus and Tropism of Electrospun Scaffolds Facilitates Altered BMSCs Proliferation and Differentiation

et al. 2023

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engineering and stem cell therapy. [3][4][5] There is increasing evidence that biomaterial surface topography is an essential factor that selectively influences cell adhesion and, in some cases, promotes cell differentiation. [6] To date, various biomaterials with different topographies at both the micrometer and nanometer scales, such as columns, grooves, and submicron fibers, have been applied to study the influence of topography on cellular functions. [7] Electrospun submicron fibers have been extensively explored as scaffolds to manipulate cell migration owing to their unique characteristics in mimicking the hierarchical architecture of the extracellular matrix. [8] In particular, aligned electrospun submicron fibers arrays can guide and promote the directional migration of cells. [9][10][11] Therefore, submicron fibers are widely applied to influence cell behavior. [12] Studies have indicated that fibroblasts on aligned substrates align themselves parallel with their substrate and increase production of the actin and focal adhesion-related genes. [13] Studies have stained neuronal differentiation marker proteins and found that neural stem cells aligned on submicron fibers had a higher differentiation rate than cells on random submicron fibers. [14] Although traditional molecular biology approaches have clearly shown that aligned submicron fibers can promote stem cell proliferation and differentiation, the mechanistic reasons for the differences between aligned and random fibers still need to be clarified. Since compositionally, dimensionally, geometrically, and morphologically controllable nanofibrous materials can be synthesized, many nanofibrous materials have been applied in biomedicine. [15,16] However, the hierarchical architecture structure is not destined to be designed into an aligned structure, leading to incomplete cell growth, a slow differentiation rate, and excessive waste of random materials. Therefore, it is essential to clarify the difference between the aligned and random structures and to reduce this difference.Researchers have found that miRNAs are essential factors in substance cell interactions. [17] MiRNAs are a class of endogenous noncoding small RNAs that bind to the 3′ untranslated regions of their target mRNAs, [18] leading to degradation or translational repression of these mRNAs, thereby regulating various cellular functions. [19] Recent studies have shown that miRNAs play Aligned submicron fibers have played an essential role in inducing stem cell proliferation and differentiation. In this study, it is aimed to identify the differential causes of stem cell proliferation and differentiation between bone marrow mesenchymal stem cells (BMSCs) on aligned-random fibers with different elastic modulus, and to change the differential levels through a regulatory mechanism mediated by B-cell lymphoma 6 protein(BCL-6) and miRNA-126-5p(miR-126-5p). The results showed that phosphatidylinositol(4,5)bisphosphate alterations are found in the aligned fibers compared with the random fibers, which ...

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“…The calcium silicate (CS) ceramics and their composites have been regarded as potential candidates for artificial bone graft materials due to their similar mechanical properties to natural bone tissues, excellent bioactivity and biodegradable properties. [39][40][41] Our previous studies have also revealed that Mg doped CS (Mg-CS) possessed excellent biodegradability and could efficiently release Si 4+ Mg 2+ at the same time. [42] Therefore, the combination of HA microscaffold and Mg-CS could be a promising method to control the degradation performance and provide a synergistic ionic microenvironment for vascularized bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

Mg‐CS/HA Microscaffolds Display Excellent Biodegradability and Controlled Release of Si and Mg Bioactive Ions to Synergistically Promote Vascularized Bone Regeneration

Wei

Zhang

et al. 2023

Adv Materials Inter

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For bone defect repair, it is critical to utilize biomaterials with pro‐angiogenic properties to enhance osteogenesis. Hydroxyapatite (HA)‐based materials widely used in clinical applications have shown much potential for bone repair. However, their predominant calcium phosphate (CaP) composition and poor biodegradability limit their angiogenic potential and hence osteogenic efficiency of HA‐based materials. Here, a magnesium ion‐doped calcium silicate/HA composite microscaffold (Mg‐CS/HA) is fabricated to enhance angiogenesis and osteogenic efficiency for bone repair. Incorporation of CS improved the biodegradability of the Mg‐CS/HA microscaffold, which could simultaneously release Si and Mg bioactive ions during the early stage of implantation, synergistically enhancing angiogenesis and osteogenic efficiency. In co‐culture systems, the synergistic effects of Si and Mg ions promote the “osteogenesis‐angiogenesis coupling effect.” In vivo, the Mg‐CS/HA microscaffold could significantly promote reconstruction of the vascular network and bone regeneration. This study thus provides a new strategy for coordinated release of bioactive ions to achieve synergistic effects on vascularized bone regeneration by HA‐based bone implant materials.

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Enhancing the osteogenic differentiation of aligned electrospun poly(L-lactic acid) nanofiber scaffolds by incorporation of bioactive calcium silicate nanowires

Cited by 16 publications

References 48 publications

Oriented Porous Polymer Scaffolds in Tissue Engineering: A Comprehensive Review of Preparation Strategies and Applications

Oriented Porous Polymer Scaffolds in Tissue Engineering: A Comprehensive Review of Preparation Strategies and Applications

PIP2 Alteration Caused by Elastic Modulus and Tropism of Electrospun Scaffolds Facilitates Altered BMSCs Proliferation and Differentiation

Mg‐CS/HA Microscaffolds Display Excellent Biodegradability and Controlled Release of Si and Mg Bioactive Ions to Synergistically Promote Vascularized Bone Regeneration

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