Enhancing the Osteogenic Differentiation and Rapid Osseointegration of 3D Printed Ti6Al4V Implants via Nano-Topographic Modification

Wang, Hui; Zhang, Xinran; Wang, Haicheng; Zhang, Jinkai; Li, Jia; Ruan, Changshun; Zhu, Rui; Lin, Kaili

doi:10.1166/jbn.2018.2551

Cited by 27 publications

(40 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To be specific, the amorphous calcium phosphate, which was the precursor in biomineralization, could trigger mitophagy to transport intracellularly via the autophagosome and released to the extracellular matrix via exocytosis (Pei et al, 2018). In this study, with the relative expression of mitophagy-related gene upregulated at 5 days, the osteogenesis-related gene expression of SLM-CO group was promoted, which was consistent with other relevant studies (Hyzy et al, 2016;H. Wang et al, 2018;Yu et al, 2018).…”

Section: Discussionsupporting

confidence: 89%

“…The hierarchical micro-nano surface topography, due to its similarity with the multi-ordered structure of human bone, had become a better choice for intrabony biomaterials (Cui et al, 2019;Zhang et al, 2019). It had been proven that the bioactivity of SLM dental implants, including in vitro mesenchymal stem cells adherence, proliferation, differentiation, and in vivo osseointegration could be improved by the hierarchical micronano topography (Ferraris et al, 2011(Ferraris et al, , 2019Gulati et al, 2017;H. Wang et al, 2018;Yu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…An up to date research had verified that the osteogenesis promoting effect of nanomodified SLM implants was strongly related to the integrin α2-PI3K-AKT signaling axis (Zheng et al, 2020). Today, SLM titanium surfaces could be nanomodified via anodization (Gulati et al, 2017), hydrothermal (H. Wang et al, 2018;Yu et al, 2018), or inorganic chemical treatments (Ferraris et al, 2011(Ferraris et al, , 2019. These surface modifications enabled additional nanotopography while preserving the nature microroughness of SLM surfaces, and consequently constituted the hierarchical micro-nano topography.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enhanced Osseointegration by the Hierarchical Micro-Nano Topography on Selective Laser Melting Ti-6Al-4V Dental Implants

Shu

Zhang

Sun

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Currently, selective laser melting (SLM) has been thriving in implant dentistry for on-demand fabricating dental implants. Based on the coarse microtopography of SLM titanium surfaces, constructing nanostructure to form the hierarchical micro-nano topography is effective in enhancing osseointegration. Given that current nanomodification techniques of SLM implants, such as anodization and hydrothermal treatment, are facing the inadequacy in costly specific apparatus and reagents, there has been no recognized nanomodified SLM dental implants. The present study aimed to construct hierarchical micro-nano topography on self-made SLM dental implants by a simple and safe inorganic chemical oxidation, and to evaluate its contribution on osteoblastic cells bioactivity and osseointegration. The surface chemical and physical parameters were characterized by FE-SEM, EDS, profilometer, AFM, and contact angle meter. The alteration on bioactivity of MG-63 human osteoblastic cells were detected by qRT-PCR. Then the osseointegration was assessed by implanting implants on the femur condyle of New Zealand Rabbits. The hierarchical micro-nano topography was constituted by the microrough surface of SLM implants and nanoneedles (diameter: 20∼50 nm, height: 150∼250 nm), after nanomodifying SLM implants in 30% hydrogen peroxide and 30% hydrochloride acid (volume ratio 1:2.5) at room temperature for 36 h. Low chemical impurities content and high hydrophilicity were observed in the nanomodified group. Cell experiments on the nanomodified group showed higher expression of mitophagy related gene (PINK1, PARKIN, LC3B, and LAMP1) at 5 days and higher expression of osteogenesis related gene (Runx2 and OCN) at 14 days. In the early stage of bone formation, the nanomodified SLM implants demonstrated higher bone-to-implant contact. Intriguingly, the initial bone-to-implant contact of nanomodified SLM implants consisted of more mineralized bone with less immature osteoid. After the cessation of bone formation, the bone-to-implant contact of nanomodified SLM implants was equal to untreated SLM implants and marketable TixOs implants. The overall findings indicated that the inorganic chemical oxidized hierarchical micro-nano topography could enhance the bioactivity of osteoblastic cells, and consequently promote the peri-implant bone formation and mineralization of SLM dental implants. This study sheds some light on improvements in additive manufactured dental implants.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhanced Osseointegration by the Hierarchical Micro-Nano Topography on Selective Laser Melting Ti-6Al-4V Dental Implants

Shu

Zhang

Sun

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Previous studies have shown that nanotopographic modification of the Ti implant can enhance osteointegration ability. 42,43 The MC3T3-E1 pre-osteoblasts were selected here as model to investigate the osteogenic activity of the surface-coated Ti disks. All four coatings allowed for the good growth, attachment, and proliferation of the cells, which had a normally flattened and wellspread morphology as well as good viability (Figure 4B and D).…”

Section: Discussionmentioning

confidence: 99%

<p>Effects of Nanotopography Regulation and Silicon Doping on Angiogenic and Osteogenic Activities of Hydroxyapatite Coating on Titanium Implant</p>

Xi¹,

Pin²,

Zhao³

et al. 2020

IJN

View full text Add to dashboard Cite

Background: Angiogenic and osteogenic activities are two major problems with biomedical titanium (Ti) and other orthopedic implants used to repair large bone defects. Purpose: The aim of this study is to prepare hydroxyapatite (HA) coatings on the surface of Ti by using electrochemical deposition (ED), and to evaluate the effects of nanotopography and silicon (Si) doping on the angiogenic and osteogenic activities of the coating in vitro. Materials and Methods: HA coating and Si-doped HA (HS) coatings with varying nanotopographies were fabricated using two ED modes, ie, the pulsive current (PC) and cyclic voltammetry (CV) methods. The coatings were characterized through scanning electron microscope (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectrometer (XPS), and atomic force microscopy (AFM), and their in vitro bioactivity and protein adsorption were assessed. Using MC3T3-E1 preosteoblasts and HUVECs as cell models, the osteogenic and angiogenic capabilities of the coatings were evaluated through in vitro cellular experiments. Results: By controlling Si content in~0.8 wt.%, the coatings resulting from the PC mode (HA-PC and HS-PC) and CV mode (HA-CV and HS-CV) had nanosheet and nanorod topographies, respectively. At lower crystallinity, higher ionic dissolution, smaller contact angle, higher surface roughness, and more negative zeta potential, the HS and PC samples exhibited quicker apatite deposition and higher BSA adsorption capacity. The in vitro cell study showed that Si doping was more favorable for enhancing the viability of the MC3T3-E1 cells, but nanosheet coating increased the area for cell spreading. Of the four coatings, HS-PC with Si doping and nanosheet topography exhibited the best effect in terms of up-regulating the expressions of the osteogenic genes (ALP, Col-I, OSX, OPN and OCN) in the MC3T3-E1 cells. Moreover, all leach liquors of the surfacecoated Ti disks promoted the growth of the HUVECs, and the HS samples played a more significant role in promoting cell migration and tube formation than the HA samples. Of the four leach liquors, only the two HS samples up-regulated NO content and expressions of the angiogenesis-related genes (VEGF, bFGF and eNOS) in the HUVECs, and the HS-PC yielded a better effect. Conclusion: The results show that Si doping while regulating the topography of the coating can help enhance the bone regeneration and vascularization of HA-coated Ti implants.

show abstract

“…UV photofunctionalization has been extensively studied in vitro and in vivo in the last decade as surface modification of implantable metallic materials (Areid et al, 2018; Henningsen et al, 2018; Soltanzadeh et al, 2017). This phenomenon has been found to be associated with the photochemical and photocatalytic removal of hydrocarbons from the metallic surfaces, and it has been suggested as an effective method of enhancing the osteoconductivity to improve the osseointegration and bone formation (Lee et al, 2019; Yamauchi et al, 2017), similar to that seen for non‐thermal plasma treatment and nano‐scaled surface modification (Guo et al, 2019; Henningsen et al, 2018; Wang et al, 2018). The treatment has proven to be effective in enhancing bone formation and bone regeneration in the treatment of critical bone defects; but, it has been mainly used in dentistry (Hirakawa et al, 2013; Park, Koak, Kim, Han, & Heo, 2013; Suzuki, Kobayashi, & Ogawa, 2013).…”

Section: Discussionmentioning

confidence: 88%

In vivo effect of UV‐photofunctionalization of CoCrMo in processes of guided bone regeneration and tissue engineering

Zuchuat

Maldonado

Botteri

et al. 2020

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

Enhancing the Osteogenic Differentiation and Rapid Osseointegration of 3D Printed Ti6Al4V Implants via Nano-Topographic Modification

Cited by 27 publications

References 0 publications

Enhanced Osseointegration by the Hierarchical Micro-Nano Topography on Selective Laser Melting Ti-6Al-4V Dental Implants

Enhanced Osseointegration by the Hierarchical Micro-Nano Topography on Selective Laser Melting Ti-6Al-4V Dental Implants

<p>Effects of Nanotopography Regulation and Silicon Doping on Angiogenic and Osteogenic Activities of Hydroxyapatite Coating on Titanium Implant</p>

In vivo effect of UV‐photofunctionalization of CoCrMo in processes of guided bone regeneration and tissue engineering

Contact Info

Product

Resources

About