Enhancing the natural folate level in wine using bioengineering and stabilization strategies

Liu, Yazheng; Walkey, Christopher J.; Green, Timothy; Vuuren, H. J. J. Van; Kitts, David D.

doi:10.1016/j.foodchem.2015.07.138

Cited by 13 publications

(7 citation statements)

References 37 publications

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“…Here we tested the effect of co-existing species, for example, sulte, polyphenolic, ascorbate acid in white grape wine. According to the previous reports, [47][48][49] the sodium sulte, gallic acid and ascorbate acid were added into two types of white grape wine, in which the added concentration of the sodium sulte, gallic acid and ascorbate acid were equal to their original concentrations, followed by collecting absorbance at 706 nm with UV-vis spectroscopy using the proposed method. For comparison, the original wine samples were adopted as the controls and detected by the proposed method too.…”

Section: Determination Of Glucose Concentration In White Grape Wine Smentioning

confidence: 99%

A colorimetric biosensor based on enzyme-catalysis-induced production of inorganic nanoparticles for sensitive detection of glucose in white grape wine

Dai

Zhang

et al. 2018

RSC Adv.

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Section: Determination Of Glucose Concentration In White Grape Wine Smentioning

confidence: 99%

A colorimetric biosensor based on enzyme-catalysis-induced production of inorganic nanoparticles for sensitive detection of glucose in white grape wine

Dai

Zhang

et al. 2018

RSC Adv.

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“…In eukaryotic microorganisms, the folate production pathway has been characterized in the model organism S. cerevisiae (Berglez et al 2005 ; Cherest et al 2000 ; Nardese et al 1996 ). In addition, this yeast has been manipulated to increase folate concentration during wine fermentation (Liu et al 2016 ; Walkey et al 2015 ). The overexpression of the endogenous gene FOL2 has been found to be the most limiting step in folate overproduction (Liu et al 2016 ; Walkey et al 2015 ).…”

Section: Metabolic Engineering For the Overproduction Of Folates In Mmentioning

confidence: 99%

“…In addition, this yeast has been manipulated to increase folate concentration during wine fermentation (Liu et al 2016 ; Walkey et al 2015 ). The overexpression of the endogenous gene FOL2 has been found to be the most limiting step in folate overproduction (Liu et al 2016 ; Walkey et al 2015 ). Folate synthesis in S. cerevisiae has also been enhanced by optimizing media composition, reaching a production of 360 μg/L (Hjortmo et al 2008 ) (Table 1 ).…”

Section: Metabolic Engineering For the Overproduction Of Folates In Mmentioning

confidence: 99%

Formation of folates by microorganisms: towards the biotechnological production of this vitamin

Revuelta

Serrano-Amatriain

Ledesma-Amaro

et al. 2018

Appl Microbiol Biotechnol

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Folates (vitamin B9) are essential micronutrients which function as cofactors in one-carbon transfer reactions involved in the synthesis of nucleotides and amino acids. Folate deficiency is associated with important diseases such as cancer, anemia, cardiovascular diseases, or neural tube defects. Epidemiological data show that folate deficiency is still highly prevalent in many populations. Hence, food fortification with synthetic folic acid (i.e., folic acid supplementation) has become mandatory in many developed countries. However, folate biofortification of staple crops and dairy products as well as folate bioproduction using metabolically engineered microorganisms are promising alternatives to folic acid supplementation. Here, we review the current strategies aimed at overproducing folates in microorganisms, in view to implement an economic feasible process for the biotechnological production of the vitamin.

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“…However, in contrast to synthetic supplements, natural folates have not been linked to increased risk of colorectal cancer [29,38,42]. Several biofortification approaches have already shown the potential of promoting folate metabolism by the metabolic engineering and upregulation of enzymes involved in folate metabolism [43][44][45][46][47][48]. However, more information about folate metabolism is needed for targeted upregulation of several folate vitamers and to increase the stability thereof.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Vitamer Profiling of Folate Mono- and Polyglutamates in Baker’s Yeast (Saccharomyces cerevisiae) as a Function of Different Sample Preparation Procedures

et al. 2020

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Folates are a group of B9 vitamins playing an important role in many metabolic processes such as methylation reactions, nucleotide synthesis or oxidation and reduction processes. However, humans are not able to synthesize folates de novo and thus rely on external sources thereof. Baker’s yeast (Saccharomyces cerevisiae) has been shown to produce high amounts of this vitamin but extensive identification of its folate metabolism is still lacking. Therefore, we optimized and compared different sample preparation and purification procedures applying solid phase extraction (SPE). Strong anion exchange (SAX), C18 and hydrophilic–lipophilic-balanced (HLB) materials were tested for their applicability in future metabolomics studies. SAX turned out to be the preferred material for the quantitative purification of folates. Qualification of several folate vitamers was achieved by ultra-high pressure liquid chromatography quadrupole time of flight mass spectrometry (UHPLC-Q-ToF-MS) measurements and quantification was performed by liquid chromatography tandem mass spectrometry (LC-MS/MS) applying stable isotope dilution assays (SIDAs). The oxidation product s-pyrazino-triazine (MeFox) was included into the SIDA method for total folate determination and validation. Applying the best protocol (SAX) in regard to folate recovery, we analyzed 32 different vitamers in different polyglutamate states up to nonaglutamates, of which we could further identify 26 vitamers based on tandem-MS (MS2) spectra. Total folate quantification revealed differences in formyl folate contents depending on the cartridge chemistry used for purification. These are supposedly a result of interconversion reactions occurring during sample preparation due to variation in pH adjustments for the different purification protocols. The occurrence of interconversion and oxidation reactions should be taken into consideration in sample preparation procedures for metabolomics analyses with a focus on folates.

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Enhancing the natural folate level in wine using bioengineering and stabilization strategies

Cited by 13 publications

References 37 publications

A colorimetric biosensor based on enzyme-catalysis-induced production of inorganic nanoparticles for sensitive detection of glucose in white grape wine

A colorimetric biosensor based on enzyme-catalysis-induced production of inorganic nanoparticles for sensitive detection of glucose in white grape wine

Formation of folates by microorganisms: towards the biotechnological production of this vitamin

Comprehensive Vitamer Profiling of Folate Mono- and Polyglutamates in Baker’s Yeast (Saccharomyces cerevisiae) as a Function of Different Sample Preparation Procedures

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