A colorimetric biosensor based on enzyme-catalysis-induced production of inorganic nanoparticles for sensitive detection of glucose in white grape wine

Dai, Houde; Li, Yuqing; Zhang, Qi; Fu, Yingchun; Li, Yanbin

doi:10.1039/c8ra06347h

Cited by 18 publications

(11 citation statements)

References 48 publications

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“…36). To evaluate the effect of the grafting on GOx, a recently developed methodology entailing the enzyme induced formation of Prussian blue nanoparticles was employed as a simple colorimetric assay 70 . Interestingly, the GOx nanoparticles showed increased activity as compared to that of either the native GOx or the macroinitiator GOx-Br, suggesting that the grafting under UV did not affect GOx and, most possibly, the interaction with the polymer or the assembly itself enhanced protein activity ( Fig.…”

Section: Articlementioning

confidence: 99%

Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach

et al. 2020

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The immense application potential of amphiphilic protein-polymer conjugates remains largely unexplored, as established "grafting from" synthetic protocols involve time-consuming, harsh and disruptive deoxygenation methods, while "grafting to" approaches result in low yields. Here we report an oxygen tolerant, photoinduced CRP approach which readily affords quantitative yields of protein-polymer conjugates within 2 h, avoiding damage to the secondary structure of the protein and providing easily accessible means to produce biomacromolecular assemblies. Importantly, our methodology is compatible with multiple proteins (e.g. BSA, HSA, GOx, beta-galactosidase) and monomer classes including acrylates, methacrylates, styrenics and acrylamides. The polymerizations are conveniently conducted in plastic syringes and in the absence of any additives or external deoxygenation procedures using low-organic content media and ppm levels of copper. The robustness of the protocol is further exemplified by its implementation under UV, blue light or even sunlight irradiation as well as in buffer, nanopure, tap or even sea water.

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Section: Articlementioning

confidence: 99%

Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach

et al. 2020

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“…Colorimetric transducers are another type of optical biosensors that act on the basis of changes in the wavelengths within the visible spectrum and have the advantages of rapidity and being observed with the naked eye [ 92 ]. Gold nanoparticles and silver nanoparticles are commonly used in colorimetric assays due to the straightforward distinction of their color transformation [ 93 ].…”

Section: Classification Of Biosensorsmentioning

confidence: 99%

Emerging biosensors in detection of natural products

Piroozmand

Mohammadipanah

Faridbod

2020

Synthetic and Systems Biotechnology

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Natural products (NPs) are a valuable source in the food, pharmaceutical, agricultural, environmental, and many other industrial sectors. Their beneficial properties along with their potential toxicities make the detection, determination or quantification of NPs essential for their application. The advanced instrumental methods require time-consuming sample preparation and analysis. In contrast, biosensors allow rapid detection of NPs, especially in complex media, and are the preferred choice of detection when speed and high throughput are intended. Here, we review diverse biosensors reported for the detection of NPs. The emerging approaches for improving the efficiency of biosensors, such as microfluidics, nanotechnology, and magnetic beads, are also discussed. The simultaneous use of two detection techniques is suggested as a robust strategy for precise detection of a specific NP with structural complexity in complicated matrices. The parallel detection of a variety of NPs structures or biological activities in a mixture of extract in a single detection phase is among the anticipated future advancements in this field which can be achieved using multisystem biosensors applying multiple flow cells, sensing elements, and detection mechanisms on miniaturized folded chips.

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“…We envision that the photothermal microfluidic sensing platform can be applied in a broad range of fields, such as environmental monitoring, food safety inspection, and medical diagnosis. For example, a great number of conventional off-chip spectroscopic and colorimetric assays employing 3,3′,5,5′-tetramethylbenzidine (TMB), gold nanorods, , and PB , as the sensing probes that have also been developed as photothermal agents ,,, can be flexibly introduced into this sensing platform to enable the multiplexed visual quantitative detection of various analytes. Hence, the photothermal microfluidic sensing platform shows great potential for POCT.…”

Section: Discussionmentioning

confidence: 99%

Section: ■ Conclusionmentioning

confidence: 99%

Photothermal Microfluidic Sensing Platform Using Near-Infrared Laser-Driven Multiplexed Dual-Mode Visual Quantitative Readout

Zhu

et al. 2019

Anal. Chem.

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The application of different sensing principles in microfluidic devices opens up further possibilities for the development of point-of-care testing (POCT). Herein, the photothermal sensing principle is introduced in microfluidic paper-based analytical devices (μPADs) to develop a photothermal microfluidic sensing platform using near-infrared (NIR) laser-driven multiplexed dual-mode visual quantitative readout. Prussian blue (PB) as the analyte-associated photothermal agent was in situ synthesized in thermoresponsive poly(N-isopropylacrylamide) hydrogels to serve as the on-chip photothermal sensing element. The NIR laser-driven photothermal effect of PB triggered not only on-chip dose-dependent heat generation but also phase transition-induced dye release from the hydrogels, simultaneously enabling both thermal image- and distance-based dual-mode visual quantitative readout of the analyte concentration in a multiplexed manner. Both the on-chip temperature elevation value of the hydrogels and the traveling distance of released dye solutions were proportional to the concentration of PB. With the detection of silver ions in environmental water as a proof-of-concept study, the photothermal μPAD can detect silver ions at a concentration as low as 0.25 μM with high selectivity and satisfactory accuracy. The photothermal microfluidic sensing platform holds great potential for POCT with promising integratability and broad applicability, owing to the combination of synergistic advantages of the photothermal sensing principle, μPADs, and photothermally responsive hydrogels.

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A colorimetric biosensor based on enzyme-catalysis-induced production of inorganic nanoparticles for sensitive detection of glucose in white grape wine

Abstract: Sensitive and selective colorimetric sensors have come into a high demand due to their simplicity, rapidity, precision and use of common laboratory instruments.

Cited by 18 publications

References 48 publications

Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach

Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach

Emerging biosensors in detection of natural products

Photothermal Microfluidic Sensing Platform Using Near-Infrared Laser-Driven Multiplexed Dual-Mode Visual Quantitative Readout

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