Enhancing the internal plant colonization rate with endophytic nitrogen-fixing bacteria

Kovtunovych, Gennadiy; Lar, O. V.; Kordyum, V. A.; Kleiner, Diethelm; Kozyrovska, Natalia

doi:10.7124/bc.00052a

Cited by 8 publications

(9 citation statements)

References 19 publications

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“…Kovtunovych et al . found a high degree of correlation between the activity of cell wall depolymerizing enzyme pectate lyase and internal colonisation of wheat roots by Klebsiella spp. The enhanced activity of cell wall hydrolyzing enzymes in the root tissues of cyanobacteria inoculated plants, ranging from 3 to 40% over uninoculated control, can be attributed to either stimulation of the plant/cyanobacteria‐related enzymes or a combination of both.…”

Section: Discussionmentioning

confidence: 95%

“…The plant cell wall exhibits a complex chemical structure, made up a network of polysaccharides, proteins, and other chemical molecules; this represents a considerable challenge to microorganisms, as it greatly restricts enzymatic attack. One of the predicted routes of bacterial penetration into plant tissues, besides mechanical/physical modes, is the secretion of an extensive repertoire of glycoside hydrolases, lyases, and esterases which can target the numerous linkages, as a means of cell wall degradation, especially in pathogens . Inoculation of plants with microorganisms elicits enhanced response levels of antioxidant machinery, PR proteins or defense enzymes or volatiles , which serve to protect the plant from salinity or pathogens and pests.…”

Section: Discussionmentioning

confidence: 99%

“…The high correlation between CMCase activity and chlorophyll accumulation in roots and shoots, and dry weight/fresh weight illustrates the direct role of this enzyme in promoting the proliferation and colonization of cyanobacteria in the plant tissues. Similarly, b-1,3-endoglucanases have been found to be involved, not only in fungal cell wall degradation, but also in several developmental and physiological processes such as pollen and seed germination [34][35][36][37]. Kovtunovych et al [34] found a high degree of correlation between the activity of cell wall depolymerizing enzyme pectate lyase and internal colonisation of wheat roots by Klebsiella spp.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, b-1,3-endoglucanases have been found to be involved, not only in fungal cell wall degradation, but also in several developmental and physiological processes such as pollen and seed germination [34][35][36][37]. Kovtunovych et al [34] found a high degree of correlation between the activity of cell wall depolymerizing enzyme pectate lyase and internal colonisation of wheat roots by Klebsiella spp. The enhanced activity of cell wall hydrolyzing enzymes in the root tissues of cyanobacteria inoculated plants, ranging from 3 to 40% over uninoculated control, can be attributed to either stimulation of the plant/cyanobacteria-related enzymes or a combination of both.…”

Section: Discussionmentioning

confidence: 99%

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Deciphering the factors associated with the colonization of rice plants by cyanobacteria

Bidyarani

Prasanna

Chawla

et al. 2014

J. Basic Microbiol.

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Cyanobacteria-rice plant interactions were analyzed using a hydroponics experiment. The activity of plant defense and pathogenesis-related enzymes, scanning electron microscopy, growth, nitrogen fixation (measured as ARA), and DNA fingerprinting assays proved useful in illustrating the nature of associations of cyanobacteria with rice plants. Microscopic analyses revealed the presence of short filaments and coiled masses of filaments of cyanobacteria near the epidermis and cortex of roots and shoot tissues. Among the six cyanobacterial strains employed, Calothrix sp. (RPC1), Anabaena laxa (RPAN8), and Anabaena azollae (C16) were the best performing strains, in terms of colonization in roots and stem. These strains also enhanced nitrogen fixation and stimulated the activity of plant defense/cell wall-degrading enzymes. A significantly high correlation was also recorded between the elicited plant enzymes, growth, and ARA. DNA fingerprinting using highly iterated palindromic sequences (HIP-TG) further helped in proving the establishment of inoculated organisms in the roots/shoots of rice plants. This study illustrated that the colonization of cyanobacteria in the plant tissues is facilitated by increased elicitation of plant enzymes, leading to improved plant growth, nutrient mobilization, and enhanced plant fitness. Such strains can be promising candidates for developing "cyanobacteria colonized-nitrogen-fixing rice plants" in the future.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Deciphering the factors associated with the colonization of rice plants by cyanobacteria

Bidyarani

Prasanna

Chawla

et al. 2014

J. Basic Microbiol.

View full text Add to dashboard Cite

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“…strains has also been reported during plant colonization (Kovtunovych et al . ). This is the first study to report the localization and the ways of in vitro colonization, penetration and persistence of two PGPR strains inside the orchid roots, its seeds and plantlets.…”

Section: Discussionmentioning

confidence: 97%

Colonization strategy of the endophytic plant growth-promoting strains ofPseudomonas fluorescensandKlebsiella oxytocaon the seeds, seedlings and roots of the epiphytic orchid,Dendrobium nobileLindl

et al. 2017

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Construction of nitrogen-fixingKlebsiella variicolaGN02 expression vector pET28a-Lac-EGFP and its colonization ofPennisetum giganteumz.x.lin roots

Lin

Chen

Zheng

et al. 2019

Biotechnology & Biotechnological Equipment

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To explore the colonization of endophytic nitrogen-fixing bacterium Klebsiella variicola in Penniseum sinense, pET28a and the enhanced green fluorescent protein (EGFP) were used as basic elements to construct a recombinant expression vector pET28a-Lac-EGFP with the addition of starter Lac in pUC18 vector using enzymatic digestion and splicing methods. The constructed recombinant vector was transformed into K. variicola GN02 cells with electroporation method and the colonization of EGFP-tagged K. variicola GN02 in the roots of Pennisetum sp. was observed using laser confocal scanning microscopy. The results of restriction enzyme digestion and sequencing analysis indicated that pET28a-Lac-EGFP was successfully constructed and the target gene was successfully transferred into K. variicola GN02 cells. Positive colonies with obvious green fluorescence were observed under ultraviolet (UV) light. Sodium dodecyl sulphate-polyacrylamide gel-electrophoresis (SDS-PAGE) showed that expression of the EGFP-labelled target protein was also successfully induced in K. variicola GN02 cells. Root scanner and confocal microscopy showed that inoculation of K. variicola GN02 strain was more conducive to the root growth of P. sinense and K. variicola GN02 mainly colonized the endothelial layer of Pennisetum sp. roots. However, colonization with a small amount of K. variicola GN02 led to its concentration in the epidermis, middle column and ducts. Our findings suggest that the endophytic nitrogen-fixing bacterium K. variicola accumulates in plant roots and is subsequently dispersed, aggregated or even colonized in susceptible plants. Furthermore, we provided a theoretical basis for the practical used of K. variicola GN02-based microbial fertilizers in P. sinense and gramineous plants.

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Enhancing the internal plant colonization rate with endophytic nitrogen-fixing bacteria

Cited by 8 publications

References 19 publications

Deciphering the factors associated with the colonization of rice plants by cyanobacteria

Deciphering the factors associated with the colonization of rice plants by cyanobacteria

Colonization strategy of the endophytic plant growth-promoting strains ofPseudomonas fluorescensandKlebsiella oxytocaon the seeds, seedlings and roots of the epiphytic orchid,Dendrobium nobileLindl

Construction of nitrogen-fixingKlebsiella variicolaGN02 expression vector pET28a-Lac-EGFP and its colonization ofPennisetum giganteumz.x.lin roots

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