Colonization strategy of the endophytic plant growth-promoting strains ofPseudomonas fluorescensandKlebsiella oxytocaon the seeds, seedlings and roots of the epiphytic orchid,Dendrobium nobileLindl

Pavlova, Anastasia S.; Leontieva, Maria R.; Смирнова, Т. А.; Коломейцева, Г. Л.; Нетрусов, А.И.; Цавкелова, Е. А.

doi:10.1111/jam.13481

Cited by 60 publications

(31 citation statements)

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“…In addition, K. oxytoca have the capacity for nitrogen fixation and the conversion of N 2 to ammonia (Temme, Zhao, & Voigt, ). It is known that nitrogen fixation can influence plant growth (Pavlova et al., ) and insect longevity (Behar et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, K. oxytoca have the capacity for nitrogen fixation and the conversion of N 2 to ammonia (Temme, Zhao, & Voigt, 2012). It is known that nitrogen fixation can influence plant growth (Pavlova et al, 2017) and insect longevity (Behar et al 2008). Another strain of K. oxytoca isolated from the gut of Poekilocerus pictus was shown to degrade oleic acid to produce the smaller-chain fatty acids (Kingsly, Jothinathan, & Thilagaraj, 2017).…”

Section: Discussionmentioning

confidence: 99%

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Intestinal probiotics restore the ecological fitness decline of Bactrocera dorsalis by irradiation

Cai

Yao

et al. 2018

Evolutionary Applications

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The sterile insect technique (SIT) as an eco‐friendly and reliable strategy has been used to control populations of insect pests of agricultural, veterinary and human health importance. Successful applications of SIT rely on the high‐level ecological fitness of sterile males. A suitable and stable gut microbiome can contribute to the ecological fitness of insect by influencing their physiology, biochemistry and development processes. Here, we show that a shift in the gut bacterial composition and structure by sterilizing irradiation, characterized by a decrease in the major gut microbiota community Enterobacteriaceae, an expansion of the minor members (e.g., Bacillaceae) and a higher richness and diversity, is tightly linked to radiation‐induced ecological fitness (male mating competitiveness, flight capacity, survival rate and life span) decline in Bactrocera dorsalis (Hendel) sterile males. Function prediction of gut microbiota indicated that changes in microbiome taxonomy tend to drive microbiome functional shifts. A higher nutrient consumption of the flourishing minor gut microbiota may cause a decline in nutrients and energy metabolic activity of host and then result in the reduced ecological fitness of irradiated flies. Furthermore, we found that a gut bacterial strain Klebsiella oxytoca (BD177) can restore ecological fitness by improving food intake and increasing haemolymph sugar and amino acid levels of irradiated B. dorsalis flies. Our findings suggest that gut symbiont‐based probiotics can be used as agents for reversing radiation‐induced ecological fitness decrease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Intestinal probiotics restore the ecological fitness decline of Bactrocera dorsalis by irradiation

Cai

Yao

et al. 2018

Evolutionary Applications

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“…GFP has been widely used for studying plant-microbe interactions [ 37 , 38 , 61 , 62 ]. Pseudomonas , a non-endospore-forming bacterium, could colonize velamen and core parenchyma of Dendrobium nobile [ 63 ]. P. polymyxa WLY78 (azotobacter) could colonize wheat, maize, and cucumber [ 38 ], though some PGPR such as Pseudomonas used in commercial bio-fertilizers, the utilization of bio-fertilizers are still a small fraction in the agriculture industry.…”

Section: Discussionmentioning

confidence: 99%

Colonization and Maize Growth Promotion Induced by Phosphate Solubilizing Bacterial Isolates

Liu

Hao

et al. 2017

IJMS

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Phosphorus (P) limits the production of maize, one of the major food crops in China. Phosphate-solubilizing bacteria (PSB) have the capacity to solubilize phosphate complexes into plant absorbable and utilizable forms by the process of acidification, chelation, and exchange reactions. In this study, six bacteria, including one Paenibacillus sp. B1 strain, four Pseudomonas sp. strains (B10, B14, SX1, and SX2) and one Sphingobium sp. SX14 strain, were those isolated from the maize rhizosphere and identified based on their 16S rRNA sequences. All strains could solubilize inorganic P (Ca3(PO4)2, FePO4 and AlPO4), and only B1 and B10 organic P (lecithin). All strains, except of SX1, produced IAA, and SX14 and B1 showed the highest level. B1 incited the highest increase in root length and the second increase in shoot and total dry weight, shoot length, and total P and nitrogen (N), along with increased root length. In addition, by confocal laser scanning microscopy (CLSM), we found that green fluorescent protein (GFP)-labeled B1 mainly colonized root surfaces and in epidermal and cortical tissue. Importantly, B1 can survive through forming spores under adverse conditions and prolong quality guarantee period of bio-fertilizer. Therefore, it can act as a good substitute for bio-fertilizer to promote agricultural sustainability.

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“…Klebsiella oxytoca (formerly known as Klebsiella pneumoniae or Aerobacter aerogenes ) is a free-living bacterium isolated from soils [ 3 ], plants and animals, including humans. K. oxytoca strains can be either pathogenic [ 5 ] or beneficial having roles, for instance, as plant growth promoters [ 3 , 6 ] and as valuable biochemical-compound producers [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Genomic traits of Klebsiella oxytoca DSM 29614, an uncommon metal-nanoparticle producer strain isolated from acid mine drainages

et al. 2018

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BackgroundKlebsiella oxytoca DSM 29614 - isolated from acid mine drainages - grows anaerobically using Fe(III)-citrate as sole carbon and energy source, unlike other enterobacteria and K. oxytoca clinical isolates. The DSM 29614 strain is multi metal resistant and produces metal nanoparticles that are embedded in its very peculiar capsular exopolysaccharide. These metal nanoparticles were effective as antimicrobial and anticancer compounds, chemical catalysts and nano-fertilizers.ResultsThe DSM 29614 strain genome was sequenced and analysed by a combination of in silico procedures. Comparative genomics, performed between 85 K. oxytoca representatives and K. oxytoca DSM 29614, revealed that this bacterial group has an open pangenome, characterized by a very small core genome (1009 genes, about 2%), a high fraction of unique (43,808 genes, about 87%) and accessory genes (5559 genes, about 11%). Proteins belonging to COG categories “Carbohydrate transport and metabolism” (G), “Amino acid transport and metabolism” (E), “Coenzyme transport and metabolism” (H), “Inorganic ion transport and metabolism” (P), and “membrane biogenesis-related proteins” (M) are particularly abundant in the predicted proteome of DSM 29614 strain. The results of a protein functional enrichment analysis - based on a previous proteomic analysis – revealed metabolic optimization during Fe(III)-citrate anaerobic utilization. In this growth condition, the observed high levels of Fe(II) may be due to different flavin metal reductases and siderophores as inferred form genome analysis. The presence of genes responsible for the synthesis of exopolysaccharide and for the tolerance to heavy metals was highlighted too. The inferred genomic insights were confirmed by a set of phenotypic tests showing specific metabolic capability in terms of i) Fe2+ and exopolysaccharide production and ii) phosphatase activity involved in precipitation of metal ion-phosphate salts.ConclusionThe K. oxytoca DSM 29614 unique capabilities of using Fe(III)-citrate as sole carbon and energy source in anaerobiosis and tolerating diverse metals coincides with the presence at the genomic level of specific genes that can support i) energy metabolism optimization, ii) cell protection by the biosynthesis of a peculiar exopolysaccharide armour entrapping metal ions and iii) general and metal-specific detoxifying activities by different proteins and metabolites.Electronic supplementary materialThe online version of this article (10.1186/s12866-018-1330-5) contains supplementary material, which is available to authorized users.

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Colonization strategy of the endophytic plant growth-promoting strains ofPseudomonas fluorescensandKlebsiella oxytocaon the seeds, seedlings and roots of the epiphytic orchid,Dendrobium nobileLindl

Abstract: This study enlarges our knowledge on orchid-microbial interactions, and provides new features on application of the nonorchid PGPR in orchid seed germination and conservation.

Cited by 60 publications

References 48 publications

Intestinal probiotics restore the ecological fitness decline of Bactrocera dorsalis by irradiation

Intestinal probiotics restore the ecological fitness decline of Bactrocera dorsalis by irradiation

Colonization and Maize Growth Promotion Induced by Phosphate Solubilizing Bacterial Isolates

Genomic traits of Klebsiella oxytoca DSM 29614, an uncommon metal-nanoparticle producer strain isolated from acid mine drainages

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