Enhancing survival, engraftment, and osteogenic potential of mesenchymal stem cells

García-Sánchez, Daniel; Fernández, Darío; Rodríguez‐Rey, José C.; Pérez-Campo, Flor M.

doi:10.4252/wjsc.v11.i10.748

Cited by 63 publications

(45 citation statements)

References 155 publications

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“…This low oxygen tension is also seen locally after musculoskeletal trauma and fracture, and in other instances of inflammation. Hypoxic conditions locally are important determinants of the subsequent function of osteogenic and vasculogenic progenitor cells (Volkmer et al, 2010 ; Garcia-Sanchez et al, 2019 ). The cellular response to hypoxia is generally controlled by the transcription factor hypoxia-inducible factor (HIF)-1.…”

Section: Methods To Enhance the Function Of Harvested Cells ( mentioning

confidence: 99%

Modifying MSC Phenotype to Facilitate Bone Healing: Biological Approaches

Goodman

Lin

2020

Front. Bioeng. Biotechnol.

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Healing of fractures and bone defects normally follows an orderly series of events including formation of a hematoma and an initial stage of inflammation, development of soft callus, formation of hard callus, and finally the stage of bone remodeling. In cases of severe musculoskeletal injury due to trauma, infection, irradiation and other adverse stimuli, deficient healing may lead to delayed or non-union; this results in a residual bone defect with instability, pain and loss of function. Modern methods of mechanical stabilization and autologous bone grafting are often successful in achieving fracture union and healing of bone defects; however, in some cases, this treatment is unsuccessful because of inadequate biological factors. Specifically, the systemic and local microenvironment may not be conducive to bone healing because of a loss of the progenitor cell population for bone and vascular lineage cells. Autologous bone grafting can provide the necessary scaffold, progenitor and differentiated lineage cells, and biological cues for bone reconstruction, however, autologous bone graft may be limited in quantity or quality. These unfavorable circumstances are magnified in systemic conditions with chronic inflammation, including obesity, diabetes, chronic renal disease, aging and others. Recently, strategies have been devised to both mitigate the necessity for, and complications from, open procedures for harvesting of autologous bone by using minimally invasive aspiration techniques and concentration of iliac crest bone cells, followed by local injection into the defect site. More elaborate strategies (not yet approved by the U.S. Food and Drug Administration-FDA) include isolation and expansion of subpopulations of the harvested cells, preconditioning of these cells or inserting specific genes to modulate or facilitate bone healing. We review the literature pertinent to the subject of modifying autologous harvested cells including MSCs to facilitate bone healing. Although many of these techniques and technologies are still in the preclinical stage and not yet approved for use in humans by the FDA, novel approaches to accelerate bone healing by modifying cells has great potential to mitigate the physical, economic and social burden of non-healing fractures and bone defects.

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Section: Methods To Enhance the Function Of Harvested Cells ( mentioning

confidence: 99%

Modifying MSC Phenotype to Facilitate Bone Healing: Biological Approaches

Goodman

Lin

2020

Front. Bioeng. Biotechnol.

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“…Customized scaffolds, made rapidly and efficiently by 3D printers, could easily reproduce the perfect shape for the lesion and correctly create the ideal niche for MSCs engraftment [ 40 ], and their osteogenic differentiation would be optimized by the oligostilbenes Res and Pol.…”

Section: Discussionmentioning

confidence: 99%

Bioengineering Bone Tissue with 3D Printed Scaffolds in the Presence of Oligostilbenes

et al. 2020

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Diseases determining bone tissue loss have a high impact on people of any age. Bone healing can be improved using a therapeutic approach based on tissue engineering. Scientific research is demonstrating that among bone regeneration techniques, interesting results, in filling of bone lesions and dehiscence have been obtained using adult mesenchymal stem cells (MSCs) integrated with biocompatible scaffolds. The geometry of the scaffold has critical effects on cell adhesion, proliferation and differentiation. Many cytokines and compounds have been demonstrated to be effective in promoting MSCs osteogenic differentiation. Oligostilbenes, such as Resveratrol (Res) and Polydatin (Pol), can increase MSCs osteoblastic features. 3D printing is an excellent technique to create scaffolds customized for the lesion and thus optimized for the patient. In this work we analyze osteoblastic features of adult MSCs integrated with 3D-printed polycarbonate scaffolds differentiated in the presence of oligostilbenes.

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“…Long-term engraftment of MSCs in vivo has proven to be challenging and has yet to be demonstrated, indicating MSCs may indirectly affect tendon healing. 99,100 Indeed, a role for MSCs as a cellular source of signals that promote healing of the endogenous tissue has been underscored by a recent effort to rename the cells as medicinal signaling cells. 101 As an alternative to adult stem cell-based cell therapies, studies in the last decade have shown the promise of directed stem cell differentiation approaches for cell replacement therapies to treat a variety of diseases and conditions.…”

Section: Emerging Cell-based Treatments and Engineering Strategiesmentioning

confidence: 99%

Bringing tendon biology to heel: Leveraging mechanisms of tendon development, healing, and regeneration to advance therapeutic strategies

Tsai

Nödl

Galloway

2020

Developmental Dynamics

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Tendons are specialized matrix‐rich connective tissues that transmit forces from muscle to bone and are essential for movement. As tissues that frequently transfer large mechanical loads, tendons are commonly injured in patients of all ages. Following injury, mammalian tendons heal poorly through a slow process that forms disorganized fibrotic scar tissue with inferior biomechanical function. Current treatments are limited and patients can be left with a weaker tendon that is likely to rerupture and an increased chance of developing degenerative conditions. More effective, alternative treatments are needed. However, our current understanding of tendon biology remains limited. Here, we emphasize why expanding our knowledge of tendon development, healing, and regeneration is imperative for advancing tendon regenerative medicine. We provide a comprehensive review of the current mechanisms governing tendon development and healing and further highlight recent work in regenerative tendon models including the neonatal mouse and zebrafish. Importantly, we discuss how present and future discoveries can be applied to both augment current treatments and design novel strategies to treat tendon injuries.

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Enhancing survival, engraftment, and osteogenic potential of mesenchymal stem cells

Cited by 63 publications

References 155 publications

Modifying MSC Phenotype to Facilitate Bone Healing: Biological Approaches

Modifying MSC Phenotype to Facilitate Bone Healing: Biological Approaches

Bioengineering Bone Tissue with 3D Printed Scaffolds in the Presence of Oligostilbenes

Bringing tendon biology to heel: Leveraging mechanisms of tendon development, healing, and regeneration to advance therapeutic strategies

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