Enhancing sensitivity of human herpes virus diagnosis with DNA microarrays using dendrimers

Striebel, Hans‐Martin; Birch-Hirschfeld, Eckhard; Egerer, Renate; Földes-Papp, Zeno; Tilz, Gernot P.; Stelzner, A.

doi:10.1016/j.yexmp.2004.05.004

Cited by 38 publications

(17 citation statements)

References 6 publications

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“…DNA dendrimers have been shown to provide significant signal amplification in a variety of assay formats, including DNA microarrays (9,22). Dendritic nucleic acid signal amplification molecules, or 3DNA dendrimers (Genisphere, Hatfield, PA), are complex, highly branched molecules comprised of interconnected monomeric subunits of partially doublestranded DNA (13).…”

Section: Resultsmentioning

confidence: 99%

Suspension Microarray with Dendrimer Signal Amplification Allows Direct and High-Throughput Subtyping of Listeria monocytogenes from Genomic DNA

Borucki

Reynolds²,

Call

et al. 2005

J Clin Microbiol

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Listeria monocytogenes is a significant cause of food-borne disease and mortality; therefore, epidemiological investigations of this pathogen require subtyping methods that are rapid, discriminatory, and reproducible. Although conventional microarray subtyping analysis has been shown to be both high resolution and genetically informative, it is still relatively low throughput and technically challenging. Suspension microarray technology eliminates the technical issues associated with planar microarrays and allows high-throughput subtyping of L. monocytogenes strains. In this study, a suspension array assay using dendrimer signal amplification allowed rapid and accurate serovar identification of L. monocytogenes strains using genomic DNA as a target. The ability to subtype genomic DNA without PCR amplification allows probes to be designed for many different regions within the bacterial genome and should allow high-resolution subtyping not possible with multiplex PCR.Listeria monocytogenes is a gram-positive bacterial pathogen that causes significant morbidity and mortality. Human listeriosis may occur as widespread epidemics or, more commonly, as sporadic cases (18). It is likely, however, that a significant percentage of sporadic cases are unrecognized single-source outbreaks (19). Epidemiological investigation of epidemic and sporadic cases of listeriosis requires molecular characterization to allow the identification of specific subtypes.Molecular subtyping techniques have identified two major phylogenetic divisions within the species, with division I including serotypes 1/2b, 3b, and 4b and division II consisting of serotypes 1/2a, 1/2c, 3a, and 3c (15,16). A third division consisting of serotypes 4a, 4c, and a subset of 4b strains has also been described (4, 24). Although 13 serotypes of L. monocytogenes have been described (20), only three serotypes (1/2a, 1/2b, and 4b) are responsible for the vast majority of clinical cases (23).L. monocytogenes subtypes are usually characterized by serotyping and then further subtyped using pulsed-field gel electrophoresis (10) or ribotyping. Due to the importance of L. monocytogenes epidemiology to human health, new subtyping technologies are constantly being developed in the hope of increasing the resolution, speed, and reproducibility of L. monocytogenes subtyping. The most recent examples of this are multilocus sequence subtyping (12, 17) and microarray genomic analysis (1,5,26). Recent studies have shown that a relatively simple genotyping microarray has subtyping resolution comparable to that of pulsed-field gel electrophoresis (AscI and ApaI digestion) and superior to that of multilocus sequence subtyping (six housekeeping genes) and ribotyping (3). L. monocytogenes subtyping using DNA microarrays is also genetically informative because it can identify the genes or alleles that characterize subtypes.DNA microarrays are typically composed of DNA "probes" (nucleic acids of known sequence) that are bound to a solid substrate, such as a glass microarray slide (referred...

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Section: Resultsmentioning

confidence: 99%

Suspension Microarray with Dendrimer Signal Amplification Allows Direct and High-Throughput Subtyping of Listeria monocytogenes from Genomic DNA

Borucki

Reynolds²,

Call

et al. 2005

J Clin Microbiol

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“…The increase in signal-tonoise ratio was shown in a DNA microarray designed to detect HSV. 164 Applying the same system to proteins achieved a 10-fold increase in signal with this dendrimer-based protocol compared with conventional coated surfaces. 165,166 Sensitive biosensors based on electrochemical impedance spectroscopy were developed with Tomalia-type PAMAM dendrimers and antibodies or DNA.…”

Section: Dendrimers In Diagnosticsmentioning

confidence: 89%

Dendrimers

Perisé-Barrios

Sepúlveda‐Crespo

Shcharbin

et al. 2014

Soft Nanoparticles for Biomedical Applications

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Dendrimers are polymeric macromolecules constituted of a repetitive sequence of monomers growing step-by-step from a multifunctional core in a radial iterative fashion, not by polymeric reactions. Their synthesis offers the opportunity to generate monodisperse, structure-controlled architectures resulting in utility in specific biomedical applications. Advances in the role of molecular weight and architecture on the behaviour of these dendrimers, together with recent progress in the design of biodegradable chemistries, has permitted the application of these branched polymers as antiviral drugs and in other applications outside medicine.

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“…The dendrimer-based fluorescence beacon has been used by Striebel [101], McIntyre [102] and Bradley [103] et al McIntyre et al [102] investigated the detection of matrix metalloprotease-7 in vivo, which was associated with benign intestinal tumors. The assay was based on PAMAM dendrimers labeled fluorescein (FITC) and conjugated tetramethylrhodamine (TMR) peptide ( Figure 24).…”

Section: Other Applications Of Peptide Dendrimersmentioning

confidence: 99%

New-generation biomedical materials: Peptide dendrimers and their application in biomedicine

Luo

She

et al. 2010

Sci. China Chem.

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Enhancing sensitivity of human herpes virus diagnosis with DNA microarrays using dendrimers

Cited by 38 publications

References 6 publications

Suspension Microarray with Dendrimer Signal Amplification Allows Direct and High-Throughput Subtyping of Listeria monocytogenes from Genomic DNA

Suspension Microarray with Dendrimer Signal Amplification Allows Direct and High-Throughput Subtyping of Listeria monocytogenes from Genomic DNA

Dendrimers

New-generation biomedical materials: Peptide dendrimers and their application in biomedicine

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