Enhancing Profitability of Ethanol Fermentation
through Gamma Ray Mutagenesis
of Saccharomyces cerevisiae

Arshad, Muhammad; Hussain, Tariq; Chaudhry, Navida; Sadia, Halima; Aslam, Bilal; Usman, Τahir; Abbas, Mazhar; Qureshi, Naseem A.; Nazir, Arif; Rajoka, Muhammad Ibrahim; Iqbal, Munawar

doi:10.15244/pjoes/78708

Cited by 8 publications

(5 citation statements)

References 19 publications

(22 reference statements)

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“…The untreated RY-18 dye sample showed mutagenicity up to 69% and 73%, while gamma/H 2 O 2 -treated RY-18 dye samples revealed a reduction in mutagenicity to 81.3% and 82.3%, respectively. These results showed that mutagenicity was reduced significantly when the RY-18 dye sample was treated with gamma radiation in combination with H 2 O 2 , and previous studies also support these findings [36,37].…”

Section: Mutagenicitysupporting

confidence: 76%

Degradation of Reactive Yellow 18 Using Ionizing Radiation Based Advanced Oxidation Processes: Cytotoxicity, Mutagenicity and By-Product Distribution

Shahzadi

Bokhari

Nazish³

et al. 2022

Water

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The degradation of Reactive Yellow 18 (RY-18), induced by gamma radiation in aqueous medium, was carried out as a function of gamma radiation dose (5–20 kGy) and concentration of hydrogen peroxide, the initial dye concentration and pH of the solution were optimized for the maximum degradation efficiency. Gamma radiations alone and in combination with H2O2 were used to degrade the RY-18. A degradation rate of 99% was achieved using an absorbed dose of 20 kGy, 0.6 mL H2O2 in acidic pH. Variations in the functional groups of untreated and treated RY-18 were determined by FTIR analysis. The LCMS technique was used to determine the intermediates formed during the degradation process. The cytotoxicity and mutagenicity of RY-18 were studied by hemolytic and Ames tests, respectively. There were significant reductions in cytotoxicity and mutagenicity in response to gamma radiation treatment. Cytotoxicity was reduced from 15.1% to 7.6% after treatment with a 20 kGy absorbed dose of gamma radiations with 0.6 mL H2O2. Mutagenicity was reduced by 81.3% and 82.3% against the bacterial strains TA98 and TA100 after treatment with a 20 kGy absorbed dose with 0.6 mL H2O2. The advanced oxidation process efficiency was evaluated using the byproduct formations, which were low-molecular-weight organic acid units, which through further oxidation were converted into carbon dioxide and water end products. Based on RY-18 degradation, cytotoxicity and mutagenicity reduction, the gamma radiation in combination with H2O2 has potential for the removal of dye from the effluents.

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Section: Mutagenicitysupporting

confidence: 76%

Degradation of Reactive Yellow 18 Using Ionizing Radiation Based Advanced Oxidation Processes: Cytotoxicity, Mutagenicity and By-Product Distribution

Shahzadi

Bokhari

Nazish³

et al. 2022

Water

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“…The inhibition of free radical by DPPH was calculated [11][12][13][14][15][16][17][18][19][20]. The reductive potential of the extracts was measured by direct electron donation in the reduction of Fe 3+ (CN) 6 to Fe 2+ (CN) 6 . The reaction mixture contained 1mL of garlic extract, 2.5 mL of phosphate buffer (0.2 M with pH 6.6) and 2.5 mL of potassium ferricyanide (1%).…”

Section: Methodsmentioning

confidence: 99%

“…Prehistoric records showed that medicinal plants and their extracts are being used as medicine for human beings. As compared to antibiotics and other medicines, medicinal plants are very effective with least side effects [1][2][3][4][5][6]. Many of the drugs used nowadays are directly or indirectly derived from medicinal plants.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antioxidant Potential and Cytotoxic Behavior of Different Varieties of <i>Allium sativum</i>

Anjum¹,

Shahid²,

Jilani³

et al. 2020

Pol. J. Environ. Stud.

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The study focused on investigation of antioxidant potential of different extracts of immature and mature garlic bulbs. Antioxidant activity was assessed by measuring the reducing power, scavenging activity of extracts of Allium sativum on 2,2-diphenyl-1-picrylhydrazyl (DPPH). Total phenolic and flavonoid contents were also checked. The results showed that garlic had significant antioxidant potential. Vegetative garlic had more antioxidant potential compared to old mature garlic except in TPC. Sulphur compounds, flavonoids and phenolic compounds are responsible for antioxidant activity of garlic. Due to high antioxidant potential, garlic can protect DNA from destruction to some extent.

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“…Fifth, reactive oxygen species in the fermentation environment result in reactive oxygen free radical accumulation that can lead to oxidative stress and other negative effects. To overcome these stresses, a series of physical, chemical, and molecular biological methods have been rapidly developed to enhance yeast robustness, including Ethidium bromide (EB) chemical treatment [9], UV-Nitroguanidine (UV-NTG) treatment [10], atmospheric room temperature plasma (ARTP) mutagenesis [11], γ-ray mutagenesis [12], protoplast fusion technology [13], adoptive evolution engineering [14], global transcription machinery engineering technology (gTME) [15] multiplex genome engineering using CRISPR/Cas9 [16], and RNAi-assisted genome evolution engineering [17]. Some S. cerevisiae mutant strains with higher ethanol synthesis ability were obtained.…”

Section: Introductionmentioning

confidence: 99%

A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis

Wang

Xie

et al. 2022

Processes

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Saccharomyces cerevisiae is an important microbial organization involved in ethanol synthesis. Mutant strains that can withstand multiple pressures during this process are critical to the industrial development of biofuels. In this study, a dual high-throughput screening method of Triphenyl-2H-tetrazoliumchloride (TTC)-based macroscopic observation and the reaction of ferric nitrate with pyruvate (or pyruvate radical ion) in fermentation broth was used. Using this, an S. cerevisiae mutant library that could tolerate 381 g/L sucrose was established by ARTP random mutation and adaptive evolution to select the best strain; its ethanol yield was increased by an additional 20.48%; and the sucrose utilization rate was 81.64%. This method is specific to the selection of strains with increased ethanol production.

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Enhancing Profitability of Ethanol Fermentation through Gamma Ray Mutagenesis of Saccharomyces cerevisiae

Cited by 8 publications

References 19 publications

Degradation of Reactive Yellow 18 Using Ionizing Radiation Based Advanced Oxidation Processes: Cytotoxicity, Mutagenicity and By-Product Distribution

Degradation of Reactive Yellow 18 Using Ionizing Radiation Based Advanced Oxidation Processes: Cytotoxicity, Mutagenicity and By-Product Distribution

Evaluation of Antioxidant Potential and Cytotoxic Behavior of Different Varieties of <i>Allium sativum</i>

A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis

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