Enhancing osteoblast functions on biofilm-contaminated titanium alloy by concentration-dependent use of methylene blue-mediated antimicrobial photodynamic therapy

Huang, Tsun-Chin; Chen, Chun‐Ju; Chen, Chuncheng; Ding, Shinn-Jyh

doi:10.1016/j.pdpdt.2019.05.021

Cited by 10 publications

(13 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the prevention and elimination of bacterial adhesion and colonization plays an important role in the successful implantation. PACT has been considered as a reliable adjunct to conventional therapeutic modalities, such as mechanical (curettage and root planing) and surgical (transplant) methods, or can be used alone in the peri-implantitis treatment [ 5 , 42 ]. PACT neither causes any resistance to microorganisms, nor is affected by the resistance of existing drugs [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…On the contrary, the use of cationic MB alone can reduce the growth of bacteria, but from the results of CFU assay, there was no sign of effective eradication effect, as confirmed by an earlier report [ 55 ]. MB can attach the bacterial membranes of Gram-positive and Gram-negative bacteria by interaction with the anionic region on the bacterial cell wall [ 5 ]. In addition, MB may be toxic to some extent.…”

Section: Discussionmentioning

confidence: 99%

“…After adhesion, bacteria will form microcolonies, which are then embedded within an extracellular polysaccharide matrix to construct complex three-dimensional biofilm structures that are difficult to remove [ 2 , 3 ]. More importantly, bacterial contamination endangers the osseointegration of titanium implants [ 4 , 5 ] and ultimately leads to bone loss around the implant. According to the literature [ 6 ], after 10 years of use without systematic supportive treatment, peri-implant disease has become a common clinical problem.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Synergistic Photoantimicrobial Chemotherapy of Methylene Blue-Encapsulated Chitosan on Biofilm-Contaminated Titanium

2021

Self Cite

View full text Add to dashboard Cite

Intensive efforts have been made to eliminate or substantial reduce bacterial adhesion and biofilm formation on titanium implants. However, in the management of peri-implantitis, the methylene blue (MB) photosensitizer commonly used in photoantimicrobial chemotherapy (PACT) is limited to a low retention on the implant surface. The purpose of this study was to assess enhancive effect of water-soluble quaternary ammonium chitosan (QTS) on MB retention on biofilm-infected SLA (sandblasted, large grid, and acid-etched) Ti alloy surfaces in vitro. The effectiveness of QTS + MB with different concentrations in eliminating Gram-negative A. actinomycetemcomitans or Gram-positive S. mutans bacteria was compared before and after PACT. Bacterial counting and lipopolysaccharide (LPS) detection were examined, and then the growth of human osteoblast-like MG63 cells was evaluated. The results indicated that the synergistic QTS + MB with retention ability significantly decreased the biofilm accumulation on the Ti alloy surface, which was better than the same concentration of 1 wt% methyl cellulose (MC). More importantly, the osteogenic activity of MG63 cells on the disinfected sample treated by QTS + MB-PACT modality was comparable to that of sterile Ti control, significantly higher than that by MC + MB-PACT modality. It is concluded that, in terms of improved retention efficacy, effective bacteria eradication, and enhanced cell growth, synergistically, PACT using the 100 μg/mL MB-encapsulated 1% QTS was a promising modality for the treatment of peri-implantitis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synergistic Photoantimicrobial Chemotherapy of Methylene Blue-Encapsulated Chitosan on Biofilm-Contaminated Titanium

2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The presence of bacteria on the implant surface will complicate the process of osseointegration and may even fails to osseointegrate. 23 , 24 Prevention of bacterial colonization and formation of a bacterial biofilm on implant surfaces are an increasing demand.…”

Section: Introductionmentioning

confidence: 99%

“…In vitro cell culture studies have shown that CaSi-based materials can support the attachment, proliferation, and differentiation of human bone mesenchymal stem cells 25 , 26 and human pulp cells. 24 More importantly, the newly formed bone tissue can grow on the surface of CaSi in rabbit calvarial defect 27 and minipig mandibular alveolar bone defect models. 28 It is worth mentioning that CaSi-based materials have shown antibacterial activity against a variety of bacterial strains.…”

Section: Introductionmentioning

confidence: 99%

Mechanical Biocompatibility, Osteogenic Activity, and Antibacterial Efficacy of Calcium Silicate–Zirconia Biocomposites

2021

Self Cite

View full text Add to dashboard Cite

Zirconia ceramics with high mechanical properties have been used as a load-bearing implant in the dental and orthopedic surgery. However, poor bone bonding properties and high elastic modulus remain a challenge. Calcium silicate (CaSi)-based ceramic can foster osteoblast adhesion, growth, and differentiation and facilitate bone ingrowth. This study was to prepare CaSi-ZrO 2 composites and evaluate their mechanical properties, long-term stability, in vitro osteogenic activity, and antibacterial ability. The Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) bacteria and human mesenchymal stem cells (hMSCs) were used to evaluate the antibacterial and osteogenic activities of implants in vitro, respectively. Results indicated that the three-point bending strength of ZrO 2 was 486 MPa and Young’s modulus was 128 GPa, which were much higher than those of the cortical bone. In contrast, the bending strength and modulus of 20% (201 MPa and 48 GPa, respectively) and 30% CaSi (126 MPa and 20 GPa, respectively) composites were close to the reported strength and modulus of the cortical bone. As expected, higher CaSi content implants significantly enhanced cell growth, differentiation, and mineralization of hMSCs. It is interesting to note the induction ability of CaSi in osteogenic differentiation of hMSCs even when cultured in the absence of an osteogenic differentiation medium. The composite with the higher CaSi contents exhibited the greater bacteriostatic effect against E. coli and S. aureus . In conclusion, the addition of 20 wt % CaSi can effectively improve the mechanical biocompatibility, osteogenesis, and antibacterial activity of ZrO 2 ceramics, which may be a potential choice for load-bearing applications.

show abstract

Effectiveness of mechanical and chemical decontamination methods for the treatment of dental implant surfaces affected by peri‐implantitis: A systematic review and meta‐analysis

Hart,

Wells,

Tsigarida

et al. 2024

Clinical & Exp Dental Res

View full text Add to dashboard Cite

ObjectiveTo assess which decontamination method(s) used for the debridement of titanium surfaces (disks and dental implants) contaminated with bacterial, most efficiently eliminate bacterial biofilms.Material and MethodsA systematic search was conducted in four electronic databases between January 1, 2010 and October 31, 2022. The search strategy followed the PICOS format and included only in vitro studies completed on either dental implant or titanium disk samples. The assessed outcome variable consisted of the most effective method(s)—chemical or mechanical— removing bacterial biofilm from titanium surfaces. A meta‐analysis was conducted, and data was summarized through single‐ and multi‐level random effects model (p < .05).ResultsThe initial search resulted in 5260 articles after the removal of duplicates. After assessment by title, abstract, and full‐text review, a total of 13 articles met the inclusion criteria for this review. Different decontamination methods were assessed, including both mechanical and chemical, with the most common method across studies being chlorhexidine (CHX). Significant heterogeneity was noted across the included studies. The meta‐analyses only identified a significant difference in biofilm reduction when CHX treatment was compared against PBS. The remaining comparisons did not identify significant differences between the various decontamination methods.ConclusionsThe present results do not demonstrate that one method of decontamination is superior in eliminating bacterial biofilm from titanium disk and implant surfaces.

show abstract

Enhancing osteoblast functions on biofilm-contaminated titanium alloy by concentration-dependent use of methylene blue-mediated antimicrobial photodynamic therapy

Cited by 10 publications

References 57 publications

Synergistic Photoantimicrobial Chemotherapy of Methylene Blue-Encapsulated Chitosan on Biofilm-Contaminated Titanium

Synergistic Photoantimicrobial Chemotherapy of Methylene Blue-Encapsulated Chitosan on Biofilm-Contaminated Titanium

Mechanical Biocompatibility, Osteogenic Activity, and Antibacterial Efficacy of Calcium Silicate–Zirconia Biocomposites

Effectiveness of mechanical and chemical decontamination methods for the treatment of dental implant surfaces affected by peri‐implantitis: A systematic review and meta‐analysis

Contact Info

Product

Resources

About