Enhancing Neurological Competence of Nanoencapsulated Cordyceps/Turmeric Extracts in Human Neuroblastoma SH-SY5Y Cells

Kaokaen, Palakorn; Sorraksa, Natchadaporn; Phonchai, Ruchee; Chaicharoenaudomrung, Nipha; Kunhorm, Phongsakorn; Noisa, Parinya

doi:10.1007/s12195-022-00752-w

Cited by 3 publications

(4 citation statements)

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“…A truncated differentiation paradigm was employed to capture changes during, rather than after, this phenotypic transition. Across three sub-IC 50 ( 57 ) CDY concentrations (5μM, 10μM and 20μM), limited differences in gross cellular morphology were observed relative to cells treated with ATRA only ( Supplementary Figure S3A ). Furthermore, qPCR (See Supplementary Methods for further details) of six neuronal marker genes ( FOS , NGFR , SYN2 , GRIK3 , GRIA4 and HOMER2 ) revealed minimal changes after CDY treatment, except for FOS (log 2 FC = 0.486, FDR = 0.047) and NGFR (log 2 FC = 0.556, FDR = 0.047), which were upregulated in the 5μM CDY group relative to controls ( Supplementary Figure S3B ).…”

Section: Resultsmentioning

confidence: 99%

Extension of mRNA poly(A) tails and 3′UTRs during neuronal differentiation exhibits variable association with post-transcriptional dynamics

Kiltschewskij

Harrison

Fitzsimmons

et al. 2023

Nucleic Acids Research

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Differentiation of neural progenitor cells into mature neuronal phenotypes relies on extensive temporospatial coordination of mRNA expression to support the development of functional brain circuitry. Cleavage and polyadenylation of mRNA has tremendous regulatory capacity through the alteration of mRNA stability and modulation of microRNA (miRNA) function, however the extent of utilization in neuronal development is currently unclear. Here, we employed poly(A) tail sequencing, mRNA sequencing, ribosome profiling and small RNA sequencing to explore the functional relationship between mRNA abundance, translation, poly(A) tail length, alternative polyadenylation (APA) and miRNA expression in an in vitro model of neuronal differentiation. Differential analysis revealed a strong bias towards poly(A) tail and 3′UTR lengthening during differentiation, both of which were positively correlated with changes in mRNA abundance, but not translation. Globally, changes in miRNA expression were predominantly associated with mRNA abundance and translation, however several miRNA–mRNA pairings with potential to regulate poly(A) tail length were identified. Furthermore, 3′UTR lengthening was observed to significantly increase the inclusion of non-conserved miRNA binding sites, potentially enhancing the regulatory capacity of these molecules in mature neuronal cells. Together, our findings suggest poly(A) tail length and APA function as part of a rich post-transcriptional regulatory matrix during neuronal differentiation.

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Section: Resultsmentioning

confidence: 99%

Extension of mRNA poly(A) tails and 3′UTRs during neuronal differentiation exhibits variable association with post-transcriptional dynamics

Kiltschewskij

Harrison

Fitzsimmons

et al. 2023

Nucleic Acids Research

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“…In a recent study, nanoencapsulated C. militaris extract relieved neuronal pathology in SH-SY5Y cells (human neuroblastoma cells) by significantly improving dopamine secretion and the expression of dopaminergic-specific genes such as leucine-rich repeat kinase 2 gene (LRRK2), LIM homeobox transcription factor 1 beta (LMX1B), Forkhead Box (FOXA2), engrailed homeobox 1(EN1), and nuclear receptor-related 1 protein (NURR1) [ 85 ]. In line with this, C. militaris treatment enhanced the expression of neuronal protein paired box 6 (PAX6), a crucial player in brain development and function [ 86 ], and neuron-specific class III beta-tubulin (nestin), a marker of neuronal progenitor cells in the adult brain [ 87 ], indicating the role of C. militaris in enhancing neuronal maturation.…”

Section: Neuroprotective Potential Of Cordyceps Ex...mentioning

confidence: 99%

“…In line with this, C. militaris treatment enhanced the expression of neuronal protein paired box 6 (PAX6), a crucial player in brain development and function [ 86 ], and neuron-specific class III beta-tubulin (nestin), a marker of neuronal progenitor cells in the adult brain [ 87 ], indicating the role of C. militaris in enhancing neuronal maturation. Furthermore, it reduced amyloid precursor protein (APP) secretion by promoting autophagy [ 85 ]. As autophagy helps clear Aβ and tau aggregates in brain cells [ 88 ], C. militaris is considered important in AD treatment.…”

Section: Neuroprotective Potential Of Cordyceps Ex...mentioning

confidence: 99%

“…The downregulated expression of AD-related genes presenilin 1 (PSEN1), presenilin 2 (PSEN2), and APP and the increased expression of the non-amyloidogenic pathway, ADAM metallopeptidase domain 10 (ADAM10), and sirtuin1 (SIRT1) by nanoencapsulated C . militaris extract suggest its potential in improving AD pathology at both the gene and protein levels [ 85 ].…”

Section: Neuroprotective Potential Of Cordyceps Ex...mentioning

confidence: 99%

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Unique Bioactives from Zombie Fungus (Cordyceps) as Promising Multitargeted Neuroprotective Agents

Sharma,

2023

Nutrients

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Cordyceps, also known as “zombie fungus”, is a non-poisonous mushroom that parasitizes insects for growth and development by manipulating the host system in a way that makes the victim behave like a “zombie”. These species produce promising bioactive metabolites, like adenosine, β-glucans, cordycepin, and ergosterol. Cordyceps has been used in traditional medicine due to its immense health benefits, as it boosts stamina, appetite, immunity, longevity, libido, memory, and sleep. Neuronal loss is the typical feature of neurodegenerative diseases (NDs) (Alzheimer’s disease (AD), Parkinson’s disease (PD), multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS)) and neurotrauma. Both these conditions share common pathophysiological features, like oxidative stress, neuroinflammation, and glutamatergic excitotoxicity. Cordyceps bioactives (adenosine, N6-(2-hydroxyethyl)-adenosine, ergosta-7, 9 (11), 22-trien-3β-ol, active peptides, and polysaccharides) exert potential antioxidant, anti-inflammatory, and anti-apoptotic activities and display beneficial effects in the management and/or treatment of neurodegenerative disorders in vitro and in vivo. Although a considerable list of compounds is available from Cordyceps, only a few have been evaluated for their neuroprotective potential and still lack information for clinical trials. In this review, the neuroprotective mechanisms and safety profile of Cordyceps extracts/bioactives have been discussed, which might be helpful in the identification of novel potential therapeutic entities in the future.

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Nanoencapsulated cordyceps extract enhances collagen synthesis and skin cell regeneration through antioxidation and autophagy

Upatcha

Kaokaen

Sorraksa

et al. 2023

Journal of Microencapsulation

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Enhancing Neurological Competence of Nanoencapsulated Cordyceps/Turmeric Extracts in Human Neuroblastoma SH-SY5Y Cells

Cited by 3 publications

References 50 publications

Extension of mRNA poly(A) tails and 3′UTRs during neuronal differentiation exhibits variable association with post-transcriptional dynamics

Extension of mRNA poly(A) tails and 3′UTRs during neuronal differentiation exhibits variable association with post-transcriptional dynamics

Unique Bioactives from Zombie Fungus (Cordyceps) as Promising Multitargeted Neuroprotective Agents

Nanoencapsulated cordyceps extract enhances collagen synthesis and skin cell regeneration through antioxidation and autophagy

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