Enhancing neural differentiation of induced pluripotent stem cells by conductive graphene/silk fibroin films

Niu, Yimeng; Chen, Xiaofang; Yao, Danyu; Ge, Peng; Liu, Haifeng; Fan, Yubo

doi:10.1002/jbm.a.36486

Cited by 43 publications

(20 citation statements)

References 57 publications

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“…Graphene based nanomaterials have been highlighted as promising substrates for neuronal differentiation of stem cells, neural regeneration and electrodes for neural recordings (Bei et al, 2019). Graphene enriched scaffolds demonstrated to increase neural differentiation by an enhanced growth and extension of neurons and the delivery of electrical stimulation (Niu et al, 2018). The developed research shows that the presence of the graphene affects the properties of the produced fibrous mats.…”

Section: Discussionmentioning

confidence: 95%

Manufacturing of polycaprolactone - Graphene fibers for nerve tissue engineering

Ginestra

2019

Journal of the Mechanical Behavior of Biomedical Materials

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Nanofibrous structures have morphological similarities to extracellular matrix and have been considered as candidate scaffolds in tissue engineering. Scaffolds made from electrospun fibers have potential in cell adhesion, proliferation and cell function. In this study, different percentages of graphene have been dispersed in a polycaprolactone-cyclopentanone solution to produce electrospun fibers. The microstructure and morphology of the fibers and the mechanical behavior of the electrospun systems were evaluated to analyze the influence of graphene content on the performances of the fibers. A significant dimensional difference between the fibers diameters of was obtained due to the graphene percentage. Accordingly, the mechanical properties of the fibrous systems are found to be influenced by the presence of the graphene. Rat stem cells were cultured on the fibrous scaffolds to evaluate the effect of the arrangement of the fibers on the morphology of the cells and differentiation into neurons. In particular, a higher population of dopaminergic neurons has been identified on the fibers with a higher percentage of graphene.

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Section: Discussionmentioning

confidence: 95%

Manufacturing of polycaprolactone - Graphene fibers for nerve tissue engineering

Ginestra

2019

Journal of the Mechanical Behavior of Biomedical Materials

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“…Resilience to gap‐junction decoupling has been attributed to conductive scaffold‐mediated intercellular communication; however, nonconductive silica nanoparticles embedded in a scaffold can produce strong cardiomyocyte tissues that show synonymous resilience to conductive AuNPs scaffolds . This is a result of topography‐induced protein adhesion, a feature that often correlates with amount of dopant . The presence of nanotopographical cues has presented as more beneficial to cellular growth than conductivity.…”

Section: Discussion and Open Questions For The Use Of Conductive Tissmentioning

confidence: 99%

“…Unlike metallic nanostructures, the use of carbon allotropes for conducting scaffolds is more common in neuronal tissue engineering applications. Niu et al synthesized a silk fibroin film doped with graphene to differentiate rat iPSCs to neurons, without external stimulation. The group admirably performed extensive gene assay to quantify the performance of the scaffolds.…”

Section: Conductive Scaffolds For Neuronal Tissue Engineeringmentioning

confidence: 99%

Conductive Scaffolds for Cardiac and Neuronal Tissue Engineering: Governing Factors and Mechanisms

Burnstine‐Townley

Eshel

Amdursky

2019

Adv Funct Materials

113

102

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Tissue engineering is a promising therapeutic approach in medicine, targeting the replacement of a diseased tissue with a healthy one grown within an artificial scaffold. Due to the high prevalence of cardiac and brain‐related ailments that involve some necrosis of tissue, cardiac and neuronal tissue engineering are intensely studied fields in regenerative medicine. A growing trend in the use of conductive scaffolds for the growth of these tissues has been witnessed recently. While the results are irrefutable, the mechanism of how an electrically conducting scaffold interacts with an electroactive tissue remains has remained elusive. An up‐to‐date summary of all work done in the field is reported, with a special focus on the specific contribution of the conductive scaffold on the performance of the formed tissue. The cell–scaffold electronic interface is then explored from an electrical engineering perspective. The electronic configuration of the system and the mechanisms and governing factors controlling the ability of a conductive scaffold to support cardiac and neuronal tissues are discussed. Using several simulations, the required conductivity of the scaffold in order for it to be suitable for tissue engineering—which also depends on the nature of the charge carriers—is also discussed.

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“…The cells were immunostained with TuJ (β‐III tubulin) for neuron (red) and DAPI for nuclei (blue). Reproduced from ref . with permission from Wiley Online Library.…”

Section: Combined Application Of Graphene‐family Materials and Sfmentioning

confidence: 99%

“…Niu et al prepared a conductive film consisting of SF and graphene, and examined the effect of the film on the neural differentiation of induced pluripotent stem cells (iPSCs). [87] Signaling using immunostaining and real-time PCR assays showed that the extent of neural differentiation increased as the graphene content increased ( Figure 5). However, when the mass ratio of graphene to SF rose above 8%, iPSC differentiation was suppressed.…”

Section: Neural Tissue Engineeringmentioning

confidence: 99%

Combined Application of Graphene‐Family Materials and Silk Fibroin in Biomedicine

et al. 2019

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The main graphene‐family materials used in biomedicine include graphene, graphene oxide, and reduced graphene oxide. They have been well documented for their distinctive microstructure and excellent physicochemical properties. Although they also have some shortcomings, such as slow biodegradation and dose‐dependent biotoxicity, these problems can be solved by using them in conjunction with other biomaterials. Silk fibroin (SF), a natural polymer material with many advantageous properties, has been used widely in biomedicine. However, SF requires modification by other biomaterials to improve its mechanical properties and control its degradation rate for further use. In recent years, the combined application of graphene‐family materials and SF has increased gradually, and the effects of it on cell behavior have been preliminary investigated, such as promoting cell adhesion, proliferation, and differentiation. Further studies of the combined use have been conducted in biomedicine, including tissue engineering, biosensor, and other biomedical usage. In this paper, this combined application has been outlined and summarized, and some envisioned challenges and future perspectives have been mentioned. We hope that this review will provide some reference and inspiration for the combined application of graphene‐family materials and SF in biomedicine in the future.

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Enhancing neural differentiation of induced pluripotent stem cells by conductive graphene/silk fibroin films

Cited by 43 publications

References 57 publications

Manufacturing of polycaprolactone - Graphene fibers for nerve tissue engineering

Manufacturing of polycaprolactone - Graphene fibers for nerve tissue engineering

Conductive Scaffolds for Cardiac and Neuronal Tissue Engineering: Governing Factors and Mechanisms

Combined Application of Graphene‐Family Materials and Silk Fibroin in Biomedicine

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