Enhancing membrane modulus of giant unilamellar lipid vesicles by lateral co-assembly of amphiphilic triblock copolymers

Kang, Jeong Yi; Choi, Ikjang; Seo, Mintae; Lee, Jin Yong; Hong, Suk In; Gong, Gyeonghyeon; Shin, Song Seok; Lee, Youngbok; Kim, Jin Woong

doi:10.1016/j.jcis.2019.10.109

Cited by 24 publications

(38 citation statements)

References 43 publications

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“…110 Using triblock copolymers with a higher molecular weight and longer hydrophobic block can promote a U-shape conformation of the polymer within the hybrid membrane. 111 The entanglement induced by the U-shape conformation results in hybrid GUVs with increased stretching modulus and improved structural stability. 111 Using a PDMS-PEO diblock copolymers that self-assemble into a bilayer does indeed overcome the limitations of the triblock copolymer and when mixed with lipids, a hybrid membrane with increased lysis strain and cohesive energy density, as compared to the pure lipid GUVs, was obtained.…”

Section: Biohybrid Guvs: Membrane Properties Composition and Functiomentioning

confidence: 99%

“…111 The entanglement induced by the U-shape conformation results in hybrid GUVs with increased stretching modulus and improved structural stability. 111 Using a PDMS-PEO diblock copolymers that self-assemble into a bilayer does indeed overcome the limitations of the triblock copolymer and when mixed with lipids, a hybrid membrane with increased lysis strain and cohesive energy density, as compared to the pure lipid GUVs, was obtained. 112 Recently, it has been shown that mixing block copolymers with lipids results in formation of lipid nano-domains, which can grow into micrometre sized domains culminating in complete phase separation.…”

Section: Biohybrid Guvs: Membrane Properties Composition and Functiomentioning

confidence: 99%

“…116 However, it is important to note that formation of domains can lead to membranes with decreased toughness with destabilization occurring at the lipid/polymer boundaries. 110,111 These types of hybrid compartments not only attempt to amalgamate the top characteristics of polymers and lipids into one cohesive unit, but can also be used to design asymmetric membranes. 17 Such membranes are obtained using a 2-step procedure, where first the polymer or lipids are assembled into a giant monolayered structure followed by addition of a second monolayer of lipid or polymer, respectively.…”

Section: Biohybrid Guvs: Membrane Properties Composition and Functiomentioning

confidence: 99%

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Biomolecule–polymer hybrid compartments: combining the best of both worlds

Meyer

Abram

Craciun

et al. 2020

Phys. Chem. Chem. Phys.

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Section: Biohybrid Guvs: Membrane Properties Composition and Functiomentioning

confidence: 99%

Section: Biohybrid Guvs: Membrane Properties Composition and Functiomentioning

confidence: 99%

Section: Biohybrid Guvs: Membrane Properties Composition and Functiomentioning

confidence: 99%

See 1 more Smart Citation

Biomolecule–polymer hybrid compartments: combining the best of both worlds

Meyer

Abram

Craciun

et al. 2020

Phys. Chem. Chem. Phys.

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show abstract

“…Most of the lipid/polymer hybrid membranes were formed symmetrically [22,[37][38][39][40] and both leaflets of the membrane are alike. In the recent work of Kang et al [40], it was shown that lateral co-assembly of a small amount of amphiphilic triblock copolymers poly(ethylene oxide)-b-poly(ε-caprolactone)-b-poly(ethylene oxide) can indeed significantly improve the stretching modulus of giant unilamellar vesicles of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine. Their work also indicated that the triblock copolymers tend to form a loop-shape rather than a bridge-shape conformation [40].…”

Section: Introductionmentioning

confidence: 99%

“…In the recent work of Kang et al [40], it was shown that lateral co-assembly of a small amount of amphiphilic triblock copolymers poly(ethylene oxide)-b-poly(ε-caprolactone)-b-poly(ethylene oxide) can indeed significantly improve the stretching modulus of giant unilamellar vesicles of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine. Their work also indicated that the triblock copolymers tend to form a loop-shape rather than a bridge-shape conformation [40]. In contrast to symmetric membranes, the biological membranes frequently exhibit asymmetric distribution of constituents on two leaflets, which plays an essential role in the membrane properties and functions [41].…”

Section: Introductionmentioning

confidence: 99%

Formation of Asymmetric and Symmetric Hybrid Membranes of Lipids and Triblock Copolymers

et al. 2020

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Hybrid membranes formed by co-assembly of AxByAx (hydrophilic-hydrophobic-hydrophilic) triblock copolymers into lipid bilayers are investigated by dissipative particle dynamics. Homogeneous hybrid membranes are developed as lipids and polymers are fully compatible. The polymer conformations can be simply classified into bridge- and loop-structures in the membranes. It is interesting to find that the long-time fraction of loop-conformation ( f L ) of copolymers in the membrane depends significantly on the hydrophilic block length (x). As x is small, an equilibrium f L * always results irrespective of the initial conformation distribution and its value depends on the hydrophobic block length (y). For large x, f L tends to be time-invariant because polymers are kinetically trapped in their initial structures. Our findings reveal that only symmetric hybrid membranes are formed for small x, while membranes with stable asymmetric leaflets can be constructed with large x. The effects of block lengths on the polymer conformations, such as transverse and lateral spans ( d ⊥ and d ‖ ) of bridge- and loop-conformations, are discussed as well.

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Synthetic Cells Revisited: Artificial Cells Construction Using Polymeric Building Blocks

Maffeis,

Heuberger,

Nikoletić

et al. 2023

Advanced Science

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The exponential growth of research on artificial cells and organelles underscores their potential as tools to advance the understanding of fundamental biological processes. The bottom–up construction from a variety of building blocks at the micro‐ and nanoscale, in combination with biomolecules is key to developing artificial cells. In this review, artificial cells are focused upon based on compartments where polymers are the main constituent of the assembly. Polymers are of particular interest due to their incredible chemical variety and the advantage of tuning the properties and functionality of their assemblies. First, the architectures of micro‐ and nanoscale polymer assemblies are introduced and then their usage as building blocks is elaborated upon. Different membrane‐bound and membrane‐less compartments and supramolecular structures and how they combine into advanced synthetic cells are presented. Then, the functional aspects are explored, addressing how artificial organelles in giant compartments mimic cellular processes. Finally, how artificial cells communicate with their surrounding and each other such as to adapt to an ever‐changing environment and achieve collective behavior as a steppingstone toward artificial tissues, is taken a look at. Engineering artificial cells with highly controllable and programmable features open new avenues for the development of sophisticated multifunctional systems.

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Enhancing membrane modulus of giant unilamellar lipid vesicles by lateral co-assembly of amphiphilic triblock copolymers

Cited by 24 publications

References 43 publications

Biomolecule–polymer hybrid compartments: combining the best of both worlds

Biomolecule–polymer hybrid compartments: combining the best of both worlds

Formation of Asymmetric and Symmetric Hybrid Membranes of Lipids and Triblock Copolymers

Synthetic Cells Revisited: Artificial Cells Construction Using Polymeric Building Blocks

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