Biomolecule–polymer hybrid compartments: combining the best of both worlds

Meyer, Claire; Abram, Sarah‐Luise; Craciun, Ioana; Palivan, Cornelia G.

doi:10.1039/d0cp00693a

Cited by 32 publications

(41 citation statements)

References 158 publications

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“…The K M value of FCs was higher than the K M of the 3-layers polyDADMAC/PSS biohybrid capsules (89.12 ± 29.48 µM and 74.51 ± 26.82 µM, respectively), while both were lower than previously reported free AtzA enzyme [25]. However, our findings are incomparable to previously reported values, including V max and Kcat, because whole cells do not encompass similar enzyme concentrations nor comparable molecular crowding [51]. Overall, the biohybrid capsules containing E. coli overexpressing AtzA, demonstrated strong biodegradation activity over the range of ATZ concentrations tested (1 to 20 mg•L −1 ).…”

Section: Viability and Activity Assayscontrasting

confidence: 99%

Layer-by-Layer Encapsulation of Herbicide-Degrading Bacteria for Improved Surface Properties and Compatibility in Soils

et al. 2021

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E. coli cells overexpressing the enzyme atrazine chlorohydrolase were coated using layer-by-layer self-assembly. The polymeric coating was designed to improve the surface properties of the cells and create positively charged, ecologically safe, bio-hybrid capsules that can efficiently degrade the herbicide atrazine in soils. The physio-chemical properties of the bacteria/polymer interface were studied as a function of the polymeric composition of the shell and its thickness. Characterization of cell viability, enzyme activity, morphology, and size of the bio-capsules was done using fluorescence spectroscopy, BET and zeta potential measurements and electron microscopy imaging. Out of several polyelectrolytes, the combination of polydiallyldimethylammonium chloride and polysodium 4-styrenesulfonate improved the surface properties and activity of the cells to the greatest extent. The resulting bio-hybrid capsules were stable, well-dispersed, with a net positive charge and a large surface area compared to the uncoated bacteria. These non-viable, bio-hybrid capsules also exhibited a kinetic advantage in comparison with uncoated cells. When added to soils, they exhibited continuous activity over a six-week period and atrazine concentrations declined by 84%. Thus, the concept of layer-by-layer coated bacteria is a promising avenue for the design of new and sustainable bioremediation and biocatalytic platforms.

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Section: Viability and Activity Assayscontrasting

confidence: 99%

Layer-by-Layer Encapsulation of Herbicide-Degrading Bacteria for Improved Surface Properties and Compatibility in Soils

et al. 2021

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“…Compartmentalization is commonly used in synthetic systems, for example to encapsulate active ingredients such as drugs [61][62][63] or food additives [64,65]. In the most simple case, reagent-encompassing containers consist of drops.…”

Section: Bioinspired Materials Processingmentioning

confidence: 99%

From vesicles to materials: bioinspired strategies for fabricating hierarchically structured soft matter

Amstad

Harrington

2021

Phil. Trans. R. Soc. A.

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Certain organisms including species of mollusks, polychaetes, onychophorans and arthropods produce exceptional polymeric materials outside their bodies under ambient conditions using concentrated fluid protein precursors. While much is understood about the structure-function relationships that define the properties of such materials, comparatively less is understood about how such materials are fabricated and specifically, how their defining hierarchical structures are achieved via bottom-up assembly. Yet this information holds great potential for inspiring sustainable manufacture of advanced polymeric materials with controlled multi-scale structure. In the present perspective, we first examine recent work elucidating the formation of the tough adhesive fibres of the mussel byssus via secretion of vesicles filled with condensed liquid protein phases (coacervates and liquid crystals)—highlighting which design principles are relevant for bio-inspiration. In the second part of the perspective, we examine the potential of recent advances in drops and additive manufacturing as a bioinspired platform for mimicking such processes to produce hierarchically structured materials. This article is part of the theme issue ‘Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)’.

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“…Both the structure and functionality of polymer vesicles can be fine-tuned, making them an attractive framework for the assembly of functional compartmentalized systems. [3][4][5] In terms of size, vesicles can be classified as either small, with diameters in the hundreds of nm range, and giant ( > 1 µm). One application of giant polymer vesicles is as synthetic compartments for artificial cells and organelles.…”

Section: Introductionmentioning

confidence: 99%

Formation of giant polymer vesicles by simple double emulsification using block copolymers as the sole surfactant

et al. 2021

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Biomolecule–polymer hybrid compartments: combining the best of both worlds

Abstract: Recent advances in bio/polymer hybrid compartments in the quest to obtain artificial cells, biosensors and catalytic compartments.

Cited by 32 publications

References 158 publications

Layer-by-Layer Encapsulation of Herbicide-Degrading Bacteria for Improved Surface Properties and Compatibility in Soils

Layer-by-Layer Encapsulation of Herbicide-Degrading Bacteria for Improved Surface Properties and Compatibility in Soils

From vesicles to materials: bioinspired strategies for fabricating hierarchically structured soft matter

Formation of giant polymer vesicles by simple double emulsification using block copolymers as the sole surfactant

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