2019
DOI: 10.3390/en12050908
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Enhancing Hydrogen Production from Chlorella sp. Biomass by Pre-Hydrolysis with Simultaneous Saccharification and Fermentation (PSSF)

Abstract: Simultaneous saccharification and fermentation (SSF) and pre-hydrolysis with SSF (PSSF) were used to produce hydrogen from the biomass of Chlorella sp. SSF was conducted using an enzyme mixture consisting of 80 filter paper unit (FPU) g-biomass−1 of cellulase, 92 U g-biomass−1 of amylase, and 120 U g-biomass−1 of glucoamylase at 35 °C for 108 h. This yielded 170 mL-H2 g-volatile-solids−1 (VS), with a productivity of 1.6 mL-H2 g-VS−1 h−1. Pre-hydrolyzing the biomass at 50 °C for 12 h resulted in the production … Show more

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Cited by 27 publications
(6 citation statements)
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References 69 publications
(80 reference statements)
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“…On the other hand, fermentation at initial pH 7 and any concentration or temperature can produce VFAs with higher yield. Acetic acid and butyric acid are the main products of the fermentation of microalgae at 35 °C, similar to the main products in the study by Giang et al [53] at the same temperature, while, at operating temperature 55 °C, the main VFAs generated were acetic acid and propionic acid. High butyric acid production in batch fermentation was obtained at initial concentration of 80 and 100 g-…”
Section: Butyric Acid Productionsupporting
confidence: 79%
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“…On the other hand, fermentation at initial pH 7 and any concentration or temperature can produce VFAs with higher yield. Acetic acid and butyric acid are the main products of the fermentation of microalgae at 35 °C, similar to the main products in the study by Giang et al [53] at the same temperature, while, at operating temperature 55 °C, the main VFAs generated were acetic acid and propionic acid. High butyric acid production in batch fermentation was obtained at initial concentration of 80 and 100 g-…”
Section: Butyric Acid Productionsupporting
confidence: 79%
“…Acetic acid and butyric acid are the main products of the fermentation of microalgae at 35 °C, similar to the main products in the study by Giang et al [53] at the same temperature, while, at operating temperature 55 °C, the main VFAs generated were acetic acid and propionic acid. High butyric acid production in batch fermentation was obtained at initial concentration of 80 and 100 g- Acetic acid and butyric acid are the main products of the fermentation of microalgae at 35 • C, similar to the main products in the study by Giang et al [53] at the same temperature, while, at operating temperature 55 • C, the main VFAs generated were acetic acid and propionic acid. High butyric acid production in batch fermentation was obtained at initial concentration of 80 and 100 g-VS/L at pH 7 with concentration of butyric acid production of 4.27 g/L (0.05 g/g-VS) and 3.81 g/L (0.04 g/g-VS), respectively (Table 3) .…”
Section: Butyric Acid Productionsupporting
confidence: 79%
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“…biomass was added to glass bottles containing 50 mL of distilled water. Mixed enzymes, including cellulase (Cellic ® CTec2), glucoamylase (Dextrozyme ® GA), and alpha-amylase (Termamyl ® SC) from Novozyme, Denmark, were applied to the glass bottle in the appropriate proportions, and the conditions were set according to Giang et al [ 37 ].…”
Section: Methodsmentioning
confidence: 99%
“…This alternative provides benefits by supporting high loads of solids, reducing quickly the initial viscosity of the substrate that leads to an increase of ethanol yield [32]. Specifically, the substrate is incubated with hydrolytic enzymes in a short time, generally between 8 and 24 h. Then, the SSF proceeds when the microorganism is inoculated, improving the saccharification process due to the different optimum temperatures of the enzymes (50 • C) and the traditional fermentation yeasts (30 • C) [33,34].…”
Section: Processes For 1g and 2g Bio-ethanol Productionmentioning
confidence: 99%