Enhancing effects of retinoic acid on monoclonal antibody production of human-human hybridomas

Aotsuka, Yasuyuki; Naito, Masafumi

doi:10.1016/0008-8749(91)90121-q

Cited by 22 publications

(8 citation statements)

References 14 publications

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“…RA can induce IgA production by LPS-activated murine spleen cells but not the production of other antibody isotypes such as IgM, IgG2a, IgG2b, and IgG3 in vitro (Tokuyama and Tokuyama, 1993). Similarly, RA can promote the production of IgA and IgG monoclonal antibodies by hybridoma cells (Aotsuka and Naito, 1991). A potential mechanism for the selective effect of RA on IgA production is through cytokines such as IL-5 and IL-6 (Ballow et al, 1996).…”

Section: Ra In Regulation Of Antibody Responsesmentioning

confidence: 97%

Retinoic Acid, Immunity, and Inflammation

Kim

2011

Vitamins &Amp; Hormones

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Section: Ra In Regulation Of Antibody Responsesmentioning

confidence: 97%

Retinoic Acid, Immunity, and Inflammation

Kim

2011

Vitamins &Amp; Hormones

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“…Vitamin A supplementation not only enhanced serum IgA but also other antibody isotypes such as IgM and IgG [49]. Retinoic acid has positive effects on B cell production of IgA [51][52][53]. TGF-1 is a major factor for B cell immunoglobulin gene switch to IgA [54][55][56].…”

Section: Vitamin a And Igamentioning

confidence: 99%

Roles of Retinoic Acid in Induction of Immunity and Immune Tolerance

Kim¹

2008

EMIDDT

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It has long been recognized that vitamin A and its metabolites have immune-regulatory roles but the mechanism has been unclear. Recently, there has been a significant progress in elucidating the functions of retinoic acid in regulation of immune cell development. Retinoic acid (all-trans and 9-cis retinoic acid) is produced from the cells of the intestine such as dendritic cells and provides an intestine-specific environmental cue to differentiating immune cells. When T cells and B cells are activated in the intestine and associated-lymphoid tissues, gut homing receptors are induced on the cells in a retinoic acid and antigen-dependent manner. Retinoic acid, produced by gut dendritic cells, is also an important signal that induces IgA-producing B cells. The gut homing T cells and B cells play essential roles in protecting the digestive tract from pathogens. Retinoic acid is required also for production of mature phagocytes in bone marrow. On the other hand, retinoic acid induces a subset of FoxP3+ regulatory T cells which is important for maintaining immune tolerance in the gut. Therefore, retinoids provide both positive and negative regulatory signals to fine-control the mucosal immune system.

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“…The growth characteristics of the parent hybridoma have been analyzed and are summarized in Table 1. Subclones were generated for high secretion [3,4] and adapted to optimally grow in serum free media [36][37][38][39][40]. Antibody used for the clinical trials was generated in serum free media [35,37,38] and purified under GMP conditions.…”

Section: Clone Generationmentioning

confidence: 99%

Summary analysis of the pre-clinical and clinical results of brain tumor patients treated with pritumumab

Glassy

Hagiwara²

2009

HAB

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Pritumumab is a human IgG1 kappa antibody that has been derived from a B-cell isolated from a regional draining lymph node of a patient with cervical carcinoma. Specificity analysis of the antibody with human tissues showed the antigen, altered tumor-associated vimentin, to be highly restricted to various cancers and not normal cells and tissues. In various clinical trials in Japan 249 patients with brain cancer were treated with pritumumab. The overall response rate was between 25-30% with several survivors beyond 5-years post-treatment. The patients were on a low dose regimen of 1mg given twice a week for a course of 24 weeks for a total dose of 48 mgs per course. Pritumumab appears to be a safe and effective therapy in patients with malignant gliomas.

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Enhancing effects of retinoic acid on monoclonal antibody production of human-human hybridomas

Cited by 22 publications

References 14 publications

Retinoic Acid, Immunity, and Inflammation

Retinoic Acid, Immunity, and Inflammation

Roles of Retinoic Acid in Induction of Immunity and Immune Tolerance

Summary analysis of the pre-clinical and clinical results of brain tumor patients treated with pritumumab

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