ENHANCING EFFECT OF HUMAN THYROID STIMULATING HORMONE (hTSH) MONOCLONAL ANTIBODY IN THE hTSH IMMUNORADIOMETRIC ASSAY (IRMA) SYSTEM

Abstract

Different monoclonal antibodies, both commercial and indigenously produced, were evaluated in various combinations to optimize an immunoradiometric assay (IRMA) system for human thyroid stimulating hormone (hTSH). During these studies, it was observed that mixing one of the indigenously produced hTSH monoclonal antibody (2B11) in the hTSH IRMA system using Immunotech (Beckman Coulter, Czech Republic) kit reagents, led to an overall increase in the assay binding and sensitivity (from 0.025 mIU/L to 0.015mIU/L) of this IRMA system. This is not a general property of all monoclonal antibodies against hTSH. The mechanism for this enhancement can be attributed to the formation of a multicomponent complex.

Characterization of Therapeutic Antibodies and Related Products

“…The quantification in biological fluids of antibody therapeutics and more generally protein/peptide therapeutics in preclinical and clinical studies is most commonly carried out using enzyme linked immunosorbent assays (ELISA) − but also by other specific immuno-binding-based methods such as radioimmunoassay and immunofluorescence (Figure ) . Immunoassays offer the advantage of being high throughput sensitive and robust methods.…”

Plasmonic metal scattering immunoassay by total internal reflection scattering microscopy with nanoscale lateral resolution

“…So far, conventional immunoassays have been used in enzymelinked immunosorbent assays (ELISA), 9 some electrochemical biosensors, 10,11 chemiluminescence immunoassays, 12,13 electrochemiluminescent immunoassays, 14 immunoradiometric assays, 15 bioluminescent immunoassays, 16 and fluorescent immunoassays. 17 Another approach that is useful for protein detection is the optical imaging of single molecules or particles under an optical microscope.…”