Enhancing effect of FSH on follicular development through yolk formation and deposition in the low-yield laying chickens

Ma, Yanfen; Yao, Jinwei; Zhou, Shaojun; Mi, Yuling; Tan, Xiaohong; Zhang, Caiqiao

doi:10.1016/j.theriogenology.2020.07.012

Cited by 9 publications

(4 citation statements)

References 38 publications

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“…The follicles contain yellow yolk, indicating their development to SWFs. The preovulatory stage of rapid yolk deposition is then initiated, and the follicles develop into enormous follicles within a short time ( Ma et al, 2020 ). VLDL and vitellogenin are the main components of the egg yolk during yolk deposition and are transported to mature oocytes via VLDLR-mediated endocytosis from the space between vessels and GCs in the oocyte membrane.…”

Section: Discussionmentioning

confidence: 99%

“…VLDL and vitellogenin are the main components of the egg yolk during yolk deposition and are transported to mature oocytes via VLDLR-mediated endocytosis from the space between vessels and GCs in the oocyte membrane. Numerous studies have shown that PPAR-g regulates VLDLR expression by promoting lipid uptake and lipogenesis in adipose tissue ( Tao et al, 2010 ; Ma et al, 2020 ). Furthermore, the ability of yolk precursor formation decreases during the aging process ( Liu et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The adiponectin receptor agonist, AdipoRon, promotes reproductive hormone secretion and gonadal development via the hypothalamic-pituitary-gonadal axis in chickens

Cao²,

Ren³

et al. 2023

Poultry Science

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The adiponectin receptor agonist, AdipoRon, promotes reproductive hormone secretion and gonadal development via the hypothalamic-pituitary-gonadal axis in chickens

Cao²,

Ren³

et al. 2023

Poultry Science

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“…Rivas et al (2016) observed that after treatment with FSH (100 ng/mL) for 48 h in vitro, P4 production in granulosa cells from hierarchical follicles but not SYFs of White Leghorn hens increased significantly. Ma et al (2020) reported that the proliferation of granulosa cells in chicken prehierarchical follicles increased significantly after treated with 100 mIU/mL FSH for 72 h. In mice, increased mitochondrial activity, elevated ATP level and mtDNA copy numbers of granulosa cells were detected during the FSH‐stimulated follicle development process (Hoque et al, 2021). Similarly, in Hyline white hens, treatment of FSH (0.01 IU/mL) promoted proliferation and ATP production, and delayed cell senescence in D‐gal‐induced senescent granulosa cells (Dong et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

MFN2 regulates progesterone biosynthesis and proliferation of granulosa cells during follicle selection in hens

Hou,

Hu,

et al. 2023

Journal Cellular Physiology

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Follicle selection in hens refers to a biological process that only one small yellow follicle (SYF) is selected daily or near‐daily for following hierarchical development (from F5/F6 to F1) until ovulation. MFN2 is a kind of GTPases located on the mitochondrial outer membrane, which plays a crucial role in mitochondrial fusion. This study aimed to elucidate the role of MFN2 in proliferation and progesterone biosynthesis of granulosa cells (GCs) during follicle selection in hens. The results showed that GCs began to produce progesterone (P4) after follicle selection, accompanied with changes from multi‐layer with flat cells to single layer with cubic cells. MFN2 was detected in GCs of follicles from SYF to F1. After follicle selection, the expression level of MFN2 in GCs upregulated significantly, accompanied with increases in P4 biosynthesis, ATP production, mitochondrial DNA (mtDNA) copy numbers of granulosa cells. FSH (80 ng/mL) facilitated the effects of P4 biosynthesis and secretion, ATP production, mtDNA copy numbers, cell proliferation and the MFN2 transcription of granulosa cells from F5 (F5G) in vitro. However, FSH treatment did not promote P4 secretion in granulosa cells from SYF (SYFG) in vitro. Meanwhile, we observed that change fold of MFN2 transcription, ATP production, mtDNA copy numbers and cell proliferation rate in F5G after treatment with FSH were greater than those in SYFG. Furthermore, expression levels of MFN2 protein and messenger RNA in F5G were significantly higher than those in SYFG after treatment with FSH. P4 biosynthesis, ATP production, mtDNA copy numbers as well as cell proliferation reduced significantly in F5G with MFN2 knockdown. Oppositely, P4 biosynthesis, ATP production, mtDNA copy numbers and cell proliferation increased significantly in SYFG after the overexpression of MFN2. Our results suggest that the upregulation of MFN2 may be involved in the initiation of P4 biosynthesis, and promotion of GCs proliferation during follicle selection.

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“…It functions as the primary stimulatory factor for follicular development, GC proliferation, steroid hormone synthesis, and reproductive processes. Our previous research has indicated that exogenous FSH injection in vivo accelerates the development of prehierarchical follicles in low-yield laying chickens involving increased follicular angiogenesis [ 23 ]. However, the potential mechanism of FSH involved in the prevention of senescence in chicken ovarian follicles is still unknown.…”

Section: Introductionmentioning

confidence: 99%

Follicle-Stimulating Hormone Alleviates Ovarian Aging by Modulating Mitophagy- and Glycophagy-Based Energy Metabolism in Hens

Dong

Guo

Yang

et al. 2022

Cells

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As a predominant hormone in the reproductive axis, follicle-stimulating hormone (FSH) is known as the primary surviving factor for follicular growth. In this study, the alleviating effect of FSH on aging chicken granulosa cells (GCs) was investigated. Results showed that FSH activated mitophagy and relieved mitochondrial edema in D-gal-induced senescent GCs, which was evidenced by an increased number of mitophagosomes as well as increased mitochondria-light chain 3 (LC3) colocalization. Mitophagy activation was accompanied by the activation of the AMP-activated protein kinase (AMPK) signaling pathway. Furthermore, upregulated glycophagy was demonstrated by an increased interaction of starch-binding domain protein 1 (STBD1) with GABA type A receptor-associated protein-like 1 (GABARAPL1) in D-gal-induced senescent GCs. FSH treatment further promoted glycophagy, accompanied by PI3K/AKT activation. PI3K inhibitor LY294002 and AKT inhibitor GSK690693 attenuated the effect of FSH on glycophagy and glycolysis. The inhibition of FSH-mediated autophagy attenuated the protective effect of FSH on naturally aging GC proliferation and glycolysis. The simultaneous blockage of PI3K/AKT and AMPK signaling also abolished the positive effect of FSH on naturally senescent ovarian energy regulation. These data reveal that FSH prevents chicken ovarian aging by modulating glycophagy- and mitophagy-based energy metabolism through the PI3K/AKT and AMPK pathways.

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Enhancing effect of FSH on follicular development through yolk formation and deposition in the low-yield laying chickens

Cited by 9 publications

References 38 publications

The adiponectin receptor agonist, AdipoRon, promotes reproductive hormone secretion and gonadal development via the hypothalamic-pituitary-gonadal axis in chickens

The adiponectin receptor agonist, AdipoRon, promotes reproductive hormone secretion and gonadal development via the hypothalamic-pituitary-gonadal axis in chickens

MFN2 regulates progesterone biosynthesis and proliferation of granulosa cells during follicle selection in hens

Follicle-Stimulating Hormone Alleviates Ovarian Aging by Modulating Mitophagy- and Glycophagy-Based Energy Metabolism in Hens

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