Enhancing Collagen Mineralization with Amelogenin Peptide: Toward the Restoration of Dentin

Mukherjee, Kaushik; Visakan, Gayathri; Phark, Jin‐Ho; Moradian‐Oldak, Janet

doi:10.1021/acsbiomaterials.9b01774

Cited by 29 publications

(19 citation statements)

References 61 publications

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“…Repeated peptide applications led to multiple microscale enamel-like layers, forming an organized aprismatic enamel while imparting significant mechanical properties to treated enamel. Furthermore, application of the P26 on demineralized dentin sections prompted P26 and collagen interactions, thus promoting collagen remineralization and enhancement of the mineral density and mechanical strength of demineralized dentin (18).…”

Section: Introductionmentioning

confidence: 98%

Amelogenin Peptide-Chitosan Hydrogel for Biomimetic Enamel Regrowth

et al. 2021

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We designed synthetic peptides that have demonstrated an effective remineralization potential to restore incipient enamel decay. In order to develop a clinically viable approach we incorporated the amelogenin-derived peptides P26 and P32 into chitosan hydrogel and examined their efficacy in the remineralization of enamel. Peptides in chitosan exhibited increased stability in vitro as compared to peptides in solution at room temperature and at 37°C. Tooth models for enamel erosion (sections) and white spot lesions (blocks) were subject to periods of demineralization. Treatment groups were subjected to remineralization in artificial saliva in the presence of P26 and P32 in solution and in chitosan hydrogel (P26-CS and P32-CS). Quantitative light-induced fluorescence (QLF) was employed to analyze mineral density following demineralization and remineralization across all the treatment groups. Scanning electron microscopy and nanoindentation were used to characterize the surface structure and mechanical strength of regrown enamel. Control enamel sections treated in artificial saliva demonstrated randomly distributed, tiny, needle-shaped crystals with a low packing density and porosities displaying mineralization defects. In samples treated with P26-CS or P32-CS a denser coating of organized hydroxyapatite (HAP) crystals was formed covering the entire surfaces of demineralized enamel window. The hardness and modulus of enamel surfaces were increased after treatment with P26-CS and P32-CS with no significant difference in the mechanical properties between the two peptide hydrogels. Analysis of mineral density by QLF showed that in enamel sections P26 peptide alone or P26-CS significantly enhanced the remineralization. In enamel blocks P26 in solution had a better efficacy than P26-CS.

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Section: Introductionmentioning

confidence: 98%

Amelogenin Peptide-Chitosan Hydrogel for Biomimetic Enamel Regrowth

et al. 2021

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“… 143 An in vitro study 143 has demonstrated that collagen fibrils are capable of inducing amelogenin assembly, and guiding oriented deposition of ACP particles. Based on these findings, Moradian-Oldak et al 144 utilized an amelogenin-inspired peptide, P26, to stimulate the interactions between collagen fibrils and amelogenin and restore demineralized dentin. P26 peptide consists of two functional domains of amelogenin, and appeared as spherical assemblies in the close vicinity of collagen fibrils (Fig.…”

Section: Dentin Remineralizationmentioning

confidence: 99%

Advances in biomineralization-inspired materials for hard tissue repair

Tang

Dong

et al. 2021

Int J Oral Sci

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Biomineralization is the process by which organisms form mineralized tissues with hierarchical structures and excellent properties, including the bones and teeth in vertebrates. The underlying mechanisms and pathways of biomineralization provide inspiration for designing and constructing materials to repair hard tissues. In particular, the formation processes of minerals can be partly replicated by utilizing bioinspired artificial materials to mimic the functions of biomolecules or stabilize intermediate mineral phases involved in biomineralization. Here, we review recent advances in biomineralization-inspired materials developed for hard tissue repair. Biomineralization-inspired materials are categorized into different types based on their specific applications, which include bone repair, dentin remineralization, and enamel remineralization. Finally, the advantages and limitations of these materials are summarized, and several perspectives on future directions are discussed.

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“…For example, amelogenin-inspired bioactive peptides with retained functional domains demonstrated an effective enamel remineralization potential in situ by forming organized aprismatic enamel while imparting significant mechanical properties (Mukherjee et al 2018). They were also effective in remineralization of dentin by promoting collagen mineralization and enhancing mechanical strength of demineralized dentin (Mukherjee et al 2020). This peptide-based biomimetic translational approach still needs to be optimized for the development of clinically viable complex biomaterials.…”

Section: Biomimetic and Bioinspired Approaches To Dentin And Enamel Repairmentioning

confidence: 99%

Biomineralization of Enamel and Dentin Mediated by Matrix Proteins

Moradian‐Oldak

George

2021

J Dent Res

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Biomineralization of enamel, dentin, and bone involves the deposition of apatite mineral crystals within an organic matrix. Bone and teeth are classic examples of biomaterials with unique biomechanical properties that are crucial to their function. The collagen-based apatite mineralization and the important function of noncollagenous proteins are similar in dentin and bone; however, enamel is formed in a unique amelogenin-containing protein matrix. While the structure and organic composition of enamel are different from those of dentin and bone, the principal molecular mechanisms of protein–protein interactions, protein self-assembly, and control of crystallization events by the organic matrix are common among these apatite-containing tissues. This review briefly summarizes enamel and dentin matrix components and their interactions with other extracellular matrix components and calcium ions in mediating the mineralization process. We highlight the crystallization events that are controlled by the protein matrix and their interactions in the extracellular matrix during enamel and dentin biomineralization. Strategies for peptide-inspired biomimetic growth of tooth enamel and bioinspired mineralization of collagen to stimulate repair of demineralized dentin and bone tissue engineering are also addressed.

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Enhancing Collagen Mineralization with Amelogenin Peptide: Toward the Restoration of Dentin

Cited by 29 publications

References 61 publications

Amelogenin Peptide-Chitosan Hydrogel for Biomimetic Enamel Regrowth

Amelogenin Peptide-Chitosan Hydrogel for Biomimetic Enamel Regrowth

Advances in biomineralization-inspired materials for hard tissue repair

Biomineralization of Enamel and Dentin Mediated by Matrix Proteins

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