Enhancing anti-diabetic potential of bitter gourd juice using pectinase: A response surface methodology approach

Deshaware, Shweta; Gupta, Sumit; Variyar, Prasad S.

doi:10.1016/j.lwt.2017.08.037

Cited by 15 publications

(6 citation statements)

References 24 publications

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“…It also contains various bioactive molecules such as phenolic compounds, triterpenes, charantin, momorcharin, saponin, momordin, vicine, oleanolic acids, alkaloids, triterpene glycosides, and saponins (Grover and Yadav 2004). Phenolic compounds have been widely reported to have both high antioxidant, antidiabetic, antimicrobial, anticancer, and anti-inflammatory activities (Deshaware et al 2017). Cell suspension cultures (CSC) offer an excellent alternative for the large-scale production of phytochemicals (Sarkate et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Elicitation of silver nanoparticles enhanced the secondary metabolites and pharmacological activities in cell suspension cultures of bitter gourd

et al. 2018

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This study describes the influence of bio-synthesized silver nanoparticles (AgNPs) on phytochemicals and their pharmacological activities in the cell suspension cultures (CSC) of bitter gourd. To standardize the effect of sucrose, plant growth regulators, medium, AgNPs and growth kinetics for the biomass and bioactive compounds accumulation in CSC of bitter gourd. The medium comprising MS salts, sucrose (30 g/L) with 2,4-D (1.0 mg/L) and TDZ (0.1 mg/L) at 28 days of CSC was appropriate for biomass and bioactive compound accumulation. The contents of silver, malondialdehyde and hydrogen peroxide were highly elevated in AgNPs (10 mg/L)-elicited CSC when compared with non-elicited CSC. AgNPs (5 mg/L) elicited CSC extracts had significantly enhanced the production of total phenolic (3.5 ± 0.2 mg/g), and flavonoid (2.5 ± 0.06 mg/g) contents than in the control CSC extracts (2.5 ± 0.1 and 1.6 ± 0.05 mg/g). AgNPs (5 mg/L) elicited CSC showed a higher amount of flavonols (1822.37 µg/g), hydroxybenzoic (1713.40 µg/g) and hydroxycinnamic (1080.10 µg/g) acids than the control CSC (1199, 1394.42 and 944.52 µg/g, respectively). Because of these metabolic changes, the pharmacological activities (antioxidant, antidiabetic, antibacterial, antifungal and anticancer) were high in the AgNPs (5 mg/L)-elicited CSC extracts in bitter gourd. The study suggested the effectiveness of elicitation process in enhancing the accumulation of phenolic compounds and pharmacological activities. AgNPs-elicited CSC offered an effective and favorable in vitro method to improve the production of bioactive compounds for potential uses in pharmaceutical industries.

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Section: Introductionmentioning

confidence: 99%

Elicitation of silver nanoparticles enhanced the secondary metabolites and pharmacological activities in cell suspension cultures of bitter gourd

et al. 2018

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“…There was also a decrease (p<0.05) in activity for P. Kudriavzevii strain F2-T429-5 in T. daniellii relative to both fermentations of A. niger and Penicillium sp fermentations in T. daniellii. Aspergillus niger's production of pectinase with the highest activity further affirmed/verified its use industrially for the production of pectinases [50][51][52][53][54]. The industrial use of A. niger, therefore, indicate its preference (predilection) for the production of enzymes such as pectinases in fermentation medium containing pectin as substrate; as in the screening of fungi for pectinolytic activity with the genus Aspergillus showing the highest pectin enzyme production potential [12].…”

Section: Resultsmentioning

confidence: 96%

Pectinase Activities of Selected Fungi Grown on Agrowastes via Solid-state Fermentation

Ametefe

Lemo²,

Orji³

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Pectinases accelerate the breakdown of the glycosidic bonds in pectin into simpler forms. Pectinases in the study were produced using three extraction solvents, three fungi, and six substrates. Citrate buffer, distilled water and 0.1 M NaCl were utilized as extraction solvents. Penicillium sp, Pichia kudriavzevii F2-T429-5 and Aspergillus niger were selectively isolated from the environment and identified. The substrates include; wheat bran, banana peels, orange peels, corn cobs, Thaumatococcus daniellii (sweet prayer plant) fruit peels, and leaves in solid-state fermentation. The dinitro salicylic acid (DNS) technique was used to determine pectinase activity. In comparison to distilled water, the study found that extracting the enzyme from the fermentation medium with 0.1 M NaCl solvent resulted in considerable (p<0.05) activity. The best substrate and fungus were orange peels and Aspergillus niger, respectively. In general, when compared to the yeast Pichia kudriavzevii F2-T429-5, the molds (Penicillium sp. and Aspergillus niger) produced pectinases with higher activity. Orange peel resulted in pectinase production with significant (p<0.05) activity compared to wheat bran, banana peels, corn cobs, Thaumatococcus daniellii (sweet prayer plant) fruit peels, and leaves. Additionally, Pichia kudriavzevii F2-T429-5 in Thaumatococcus daniellii fruit peel fermentation produced pectinase with the lowest activity. The inference drawn from the study shows the potential of T. daniellii fruit peels, its leaves, and Pichia kudriavzevii F2-T429-5 for pectinase production.

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“…M. charantia L. é uma planta anual que pertence a família Cucurbitaceae e é invetigada quanto a seu potencial medicinal (Deshaware, et al, 2017). Caule, flores, frutos e sementes são utilizados na diminuição dos níveis de glicose no sangue (Fernandes, et al, 2007;Fuangchan, et al, 2011).…”

Section: Discussionunclassified

“…As condições ideais na preparação de suco a base de frutos de M. charantia L. podem ser encontradas a partir de uma superfície de resposta. A produção de 82% do suco de M. charantia L. com 710 µg de compostos fenólicos e 198 µg de conteúdo antioxidante é realizada por meio de 19,2 ml/kg de pectinase que aumenta o rendimento da polpa, 140 min de incubação e 48,8 ºC de temperatura (Deshaware, et al, 2017).…”

Section: Discussionunclassified

Momordica charantia L. no tratamento de diabetes melittus

Cruz

Freire

Pereira

et al. 2020

RSD

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Introduction: Drugs of plant origin have been used since ancient times. The use of medicinal plants in disease control has grown due to therapeutic feasibility and successful clinical cases. Some plant species are used to combat the symptoms caused by diabetes mellitus in association or not with traditional medicine, among them, the São Caetano melon (Momordica charantia L.). Objective: The objective of this literature review is to analyze the main researches on the use of M. charantia L. in the treatment of diabetes mellitus. Methodology: The work consists of an integrative literature review based on original articles in the English language from the Google Scholar, Oxford Academic, Science Direct, Taylor & Francis and Web of Science databases. Results: Of the 31 articles selected for the research, only 5 were used (16.13%). It was observed that in the 5 articles used, M. charantia L. reduced glycemic levels, as well as providing other benefits. Conclusion: The prophylactic use of M. charantia L. is an alternative in the treatment of diabetes mellitus, as well as other diseases.

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Enhancing anti-diabetic potential of bitter gourd juice using pectinase: A response surface methodology approach

Cited by 15 publications

References 24 publications

Elicitation of silver nanoparticles enhanced the secondary metabolites and pharmacological activities in cell suspension cultures of bitter gourd

Elicitation of silver nanoparticles enhanced the secondary metabolites and pharmacological activities in cell suspension cultures of bitter gourd

Pectinase Activities of Selected Fungi Grown on Agrowastes via Solid-state Fermentation

Momordica charantia L. no tratamento de diabetes melittus

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