Enhancer effect of fluorescein on the luminol-H2O2-horseradish peroxidase chemiluminescence: energy transfer process

Dı́az, A. Navas; García, J.A. González; Lovillo, J.

doi:10.1002/(sici)1099-1271(199707/08)12:4<199::aid-bio445>3.0.co;2-u

Cited by 32 publications

(9 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, we applied the Cu 2+ ‐NMOFs as a functional material for stimulating a CRET process. Previous studies indicated that the chemiluminescence generated by HRP using the luminol/H 2 O 2 system can induce, albeit at very low efficiency, a CRET process to organic dyes, e.g., fluorescein . Also, the hemin/G‐quadruplex HRP‐mimicking DNAzyme linked to semiconductor quantum dots or associated in supramolecular complexes with organic dyes leads, in the presence of luminol/H 2 O 2 , to inefficient CRET processes that result in the luminescence of the quantum dots or the fluorescence of the organic dyes, without external excitation of the chromophores.…”

Section: Comparison Of the Catalytic Performance Of Different Cu2+‐iomentioning

confidence: 99%

Cu²⁺‐Modified Metal–Organic Framework Nanoparticles: A Peroxidase‐Mimicking Nanoenzyme

Chen

Vázquez‐González

Kozell

et al. 2017

Small

145

View full text Add to dashboard Cite

Metal-organic frameworks (MOFs) represent a broad class of porous materials composed of metal ions cross-linked by organic ligands. [1] Besides the interesting structural features of MOFs, [2] many different applications of MOFs were reported [3] including their use as drug carriers, [4] catalysts, [5] sensors, [6] gas storage, [7] separation, [8] optical devices, [9] photocatalysts, [10] and as micromotors. [11] The use of the highly porous MOFs as structural scaffolds for the development of catalysts is particularly interesting. [12] Different methods to synthesize MOF-based catalysts were developed and these included the incorporation of catalytic complexes into the pores of the MOFs, [13] the incorporation of metal complexes as functional ligands of the MOF frameworks, [14] and the construction of MOFs that include ligands for the postsynthetic anchoring of metal ions and the www.advancedsciencenews.com www.small-journal.com small NANO MICRO

show abstract

Section: Comparison Of the Catalytic Performance Of Different Cu2+‐iomentioning

confidence: 99%

Cu²⁺‐Modified Metal–Organic Framework Nanoparticles: A Peroxidase‐Mimicking Nanoenzyme

Chen

Vázquez‐González

Kozell

et al. 2017

Small

145

View full text Add to dashboard Cite

show abstract

“…Our studies revealed that in the absence of an external light source, and as consequence of luminol ECL reaction, fluorescein is able to re‐emit some of the absorbed energy of the electrochemically excited‐state luminol . Moreover, in the field of enzyme‐based chemiluminescence assays, Navas et al ., also had reported that fluorescein, in presence of a luminol‐H 2 O 2 ‐peroxidase system, augmented total chemiluminescence emission . However, ECL‐RET assays in flow system are not widely exploited so far.…”

Section: Figurementioning

confidence: 96%

“…However, ECL‐RET assays in flow system are not widely exploited so far. Hence, and taking in account the excellent behavior of ECL‐RET from luminol−H 2 O 2 system to fluorescein, and the previous data regarding total luminescence increase; herein, we propose a ECL‐RET, enzyme‐free, with the intend of using the enhancement effect of fluorescein in the total luminescence of the assay, in order to increase the sensitivity of H 2 O 2 detection, in flow analysis. H 2 O 2 was chosen as analyte of interest since it is, at the same time, a co‐reactant of luminol ECL and a product of many enzymatic reactions.…”

Section: Figurementioning

confidence: 99%

“…This ECL‐RET assay, it is a concentration‐dependent emission, which continues to increase until a critical concentration value, where saturation is achieved . From this point, a decrease in fluorescent efficiency (and hence, fluorescent intensity), due to high fluorescein concentrations self‐quenching effects is registered . Thus, a fluorescein concentration value of 0.5 mM was established to perform the donor‐acceptor luminescent strategy (data not shown).…”

Section: Figurementioning

confidence: 99%

See 1 more Smart Citation

A Miniaturized Flow Injection Analysis System for Electrogenerated Chemiluminescence−Based Assays

et al. 2017

View full text Add to dashboard Cite

A new miniaturized flow injection analysis (FIA) system for developing electrochemiluminescence (ECL)-based assays is presented. Novel thin-layer flowÀcell (TLFCL) screen-printed carbon electrodes integrated in one-channel flow cells were used as the electrode surface. These TLFCL electrodes, combined with miniaturized electrochemical and optical instrumentation, allowed a high degree of automation and simplicity of the assay. The performance of this high-throughput flow system was corroborated with a proof-of-concept centered on H 2 O 2 detection. For that purpose, an ECL transduction chemistry mechanism based on a electrochemiluminescence resonance energy transfer (ECL-RET) donorÀacceptor strategy, between an electro-oxidized luminophore (luminol) to a fluorophore (fluorescein), in the presence of the analyte of interest, was carried out. The results showed that fluorescein ECL-RET luminescent response increases the sensitivity of H 2 O 2 detection by up to one order of magnitude, in comparison with a luminolÀH 2 O 2 fluorescein-free assay. All experiments were performed in a decentralized, small-sized, easy-to-use FIA system coupled to ECL detection instrumentation.The light emitted from electrogenerated chemiluminescence (ECL) is a process in which electrochemically generated species combine to undergo electron-transfer reactions to form excited, light-emitting species, giving place to the development of high sensitive and selective assays. [1][2][3][4][5][6][7][8][9][10][11] In order to increase the automation of luminescent analytical procedures, the detection of ECL response by flow injection analysis system (FIA) is an interesting option. [12,13] Flow analysis is characterized by highly reproducible and throughput sampling. In addition, sequential injection analysis allows a considerable reduction in the consumption of the sample and reagents. [14] Bearing in mind that ECL based advances are moving towards the development of portable, high-throughput and point-of-care testing systems, [8,15] disposable screen-printed electrodes coupled to a small-sized, non-conventional, FIA system appears as an attractive possibility.For that purpose, in this work, screen-printed electrodes integrated in one channel flow-cell (TLFCL, Thin layer flow-cell) were used as the electrode surface. In these electrodes a transparent slide, that usefully allows the detection of air bubbles inside the cell, is attached over the screen-printed electrodes platform delimiting a flow channel. The injection of sample volume is done through an "in-line luer" injection port, placed closest to the electrochemical cell, where the injection can be easily controlled by operator through a precise syringe. This configuration brings important advantages since it simplifies operability and effectiveness of working in FIA systems. A similar format was already reported in two previous works [16,17] where enzymatic amperometric based sensors were developed. However, this is the first time that these electrodes, integrated in the necessary accessori...

show abstract

“…Some techniques use several substances as chemiluminescent reagents at once. For instance, addition of fluorescein to the system increases the chemiluminescence intensity in the presence of luminol [ 41 ]. An increase in luminescence intensity upon addition of some phenols to the horseradish peroxidase–H 2 O 2 – luminol system was also reported [ 42 ].…”

Section: Examples Of Substances That Enhance Chemiluminescencementioning

confidence: 99%

Chemiluminescence Detection in the Study of Free-Radical Reactions. Part 2. Luminescent Additives That Increase the Chemiluminescence Quantum Yield

Romodin

2022

Acta Naturae

View full text Add to dashboard Cite

The present review examines the use of chemiluminescence detection to evaluate the course of free radical reactions in biological model systems. The application of the method is analyzed by using luminescent additives that enhance the luminescence thanks to a tripletsinglet transfer of the electron excitation energy from radical reaction products and its emission in the form of light with a high quantum yield; these additives are called chemiluminescence enhancers or activators. Examples of these substances are provided; differences between the so-called chemical and physical enhancers are described; coumarin derivatives, as the most promising chemiluminescence enhancers for studying lipid peroxidation, are considered in detail. The main problems related to the use of coumarin derivatives are defined, and possible ways of solving these problems are presented. Intrinsic chemiluminescence and the mechanism of luminescence accompanying biomolecule peroxidation are discussed in the first part of the review.

show abstract

Enhancer effect of fluorescein on the luminol-H2O2-horseradish peroxidase chemiluminescence: energy transfer process

Cited by 32 publications

References 15 publications

Cu²⁺‐Modified Metal–Organic Framework Nanoparticles: A Peroxidase‐Mimicking Nanoenzyme

Cu²⁺‐Modified Metal–Organic Framework Nanoparticles: A Peroxidase‐Mimicking Nanoenzyme

A Miniaturized Flow Injection Analysis System for Electrogenerated Chemiluminescence−Based Assays

Chemiluminescence Detection in the Study of Free-Radical Reactions. Part 2. Luminescent Additives That Increase the Chemiluminescence Quantum Yield

Contact Info

Product

Resources

About

Enhancer effect of fluorescein on the luminol-H2O2-horseradish peroxidase chemiluminescence: energy transfer process

Cited by 32 publications

References 15 publications

Cu2+‐Modified Metal–Organic Framework Nanoparticles: A Peroxidase‐Mimicking Nanoenzyme

Cu2+‐Modified Metal–Organic Framework Nanoparticles: A Peroxidase‐Mimicking Nanoenzyme

A Miniaturized Flow Injection Analysis System for Electrogenerated Chemiluminescence−Based Assays

Chemiluminescence Detection in the Study of Free-Radical Reactions. Part 2. Luminescent Additives That Increase the Chemiluminescence Quantum Yield

Contact Info

Product

Resources

About

Cu²⁺‐Modified Metal–Organic Framework Nanoparticles: A Peroxidase‐Mimicking Nanoenzyme

Cu²⁺‐Modified Metal–Organic Framework Nanoparticles: A Peroxidase‐Mimicking Nanoenzyme