Enhancement of γ-aminobutyric acid (GABA) and other health-promoting metabolites in germinated broccoli by mannose treatment

Xie, Keqin; Wu, Chenghao; Chi, Zongyu; Wang, Jing; Wang, Hongfei; Wei, Yingying; Shao, Xingfeng; Xu, Feng

doi:10.1016/j.scienta.2020.109706

Cited by 12 publications

(7 citation statements)

References 31 publications

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“…At the same time, fructose promoted GAD activity ( Figure 8 A), which induced the GABA accumulation ( Figure 3 A). This is similar to a previous study in which exogenous mannose boosted GABA accumulation in broccoli sprouts by providing more glutamate and increasing GAD activity [ 16 ]. In this study, BoGAD1 and BoGAD2 dominated with significantly higher expression levels under fructose treatment.…”

Section: Discussionsupporting

confidence: 91%

“…In this study, fructose treatment exhibited an inhibitory effect on sprout stem length and enhanced the ABA content ( Figure 1 and Figure 4 A). The findings were in keeping with the reduction in the length of broccoli sprouts treated with mannose [ 16 , 27 ]. It is suggested that a 60 mmol L −1 concentration of fructose is detrimental to the growth conditions of broccoli sprouts.…”

Section: Discussionsupporting

confidence: 72%

“…The mixture of 0.8 mL supernatant and 0.2 mL of 1 mol L −1 KOH was centrifuged at 8000× g for 5 min. Then, the glutamate content was then determined according to Xie et al [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

“…CaCl 2 -HCl electrolyzed water treatment enriched the amount of glucoraphanin and calcium contents in broccoli sprouts [ 15 ]. In our previous study, mannose treatment significantly increased the production of GABA in broccoli sprouts [ 16 ]. However, little focus has been given to the relationship between GABA metabolism and calcium in broccoli sprouts.…”

Section: Introductionmentioning

confidence: 99%

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Calcium Involved in the Enrichment of γ-Aminobutyric Acid (GABA) in Broccoli Sprouts under Fructose Treatment

Wei

Xie

Wang

et al. 2023

Plants

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The effect of fructose on γ-aminobutyric acid (GABA) content and its metabolic pathway in broccoli sprouts was investigated. The results demonstrated that the fructose treatment not only significantly increased the fresh weight, GABA, and glutamate contents in sprouts, but also promoted the activity of glutamic acid decarboxylase (GAD) and the expressions of BoGAD1 and BoGAD2. Meanwhile, fructose treatment inhibited the stem length of broccoli sprouts and enhanced the abscisic acid (ABA) production in comparison with the control. Ca2+, CaM contents, and BoCaM2 expression in broccoli sprouts were also stimulated after fructose treatment. Exogenous fructose increased inositol trisphosphate (IP3) content and activated the activity of phosphatidylinositol-specific phospholipase C (PI-PLC) and the expression of BoPLC2, contributing to Ca2+ influx into the cells. These results suggested that Ca2+ played an essential role in GABA enrichment under fructose treatment, which may be associated with GAD and PI-PLC.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 72%

“…The mixture of 0.8 mL supernatant and 0.2 mL of 1 mol L −1 KOH was centrifuged at 8000× g for 5 min. Then, the glutamate content was then determined according to Xie et al [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Calcium Involved in the Enrichment of γ-Aminobutyric Acid (GABA) in Broccoli Sprouts under Fructose Treatment

Wei

Xie

Wang

et al. 2023

Plants

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“…The results indicated that the acceleration of TCA cycle might activate GABA shunt. Previous studies revealed that in germinated broccoli mannose could enhance GABA biosynthesis and polyamine degradation (Xie et al, 2021). The similar results were observed in the embryonic axis elongation period of C. Semen.…”

Section: Analysis Of Key Metabolites and Genes During The Embryonic A...supporting

confidence: 76%

· Integrated metabolic and transcriptomic profiles reveal the germination-associated dynamic changes for Cassiae Semen

Chen

Shi

et al. 2022

Preprint

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Introduction The seeds of Cassia obtusifolia L. (Cassiae Semen) have been widely used as both food and traditional Chinese medicine in China. Objectives For better understanding the metabolic mechanism along with germination, different samples of Cassiae Semen at various germinating stages were collected. Methods These samples were subjected to 1H-NMR and UHPLC/Q-Orbitrap-MS based untargeted metabolomics analysis together with transcription analysis. Results A total of fifty differential metabolites (mainly amino acids and sugars) and twenty key genes involved in multiple pathways were identified in two comparisons of different groups (36 h vs 12 h and 84 h vs 36 h). The metabolic and gene network for seed germination was depicted. In the germination of C. Semen, the fructose and mannose metabolism pathway was activated, indicating energy was more needed in the testa rupture period (36 h). In the embryonic axis elongation period (84 h), the pentose and glucuronate interconversions pathway, and phenylpropanoid biosynthesis pathway were activated, which suggested some nutrient sources (nitrogen and sugar) would be demanded. Furthermore, oxygen, energy and nutrition should be supplied through the whole germination process. These global views open up an integrated perspective for understanding the complex biological regulatory mechanism during seed germination process of C. Semen.

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Integrated metabolic and transcriptomic profiles reveal the germination‐associated dynamic changes for the seeds of Cassia obtusifolia L.

Chen

Shi

et al. 2023

Phytochemical Analysis

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Introduction: The seeds of Cassia obtusifolia L. (Cassiae [C.] semen) have been widely used as both food and traditional Chinese medicine in China. Objectives: We aimed to analyze the metabolic mechanisms underlying C. semen germination.Materials and Methods: Different samples of C. semen at various germination stages were collected. These samples were subjected to 1 H-NMR and UHPLC/Q-Orbitrap-MS-based untargeted metabolomics analysis together with transcriptomics analysis.Results: A total of 50 differential metabolites (mainly amino acids and sugars) and 20 key genes involved in multiple pathways were identified in two comparisons of different groups (36 h vs 12 h and 84 h vs 36 h). The metabolite-gene network for seed germination was depicted. In the germination of C. semen, fructose and mannose metabolism was activated in the testa rupture period, indicating more energy was needed (36 h). In the embryonic axis elongation period (84 h), the pentose and glucuronate interconversions pathway and the phenylpropanoid biosynthesis pathway were activated, which suggested some nutrient sources (nitrogen and sugar) were in demand. Furthermore, oxygen, energy, and nutrition should be supplied throughout the whole germination process. These global views open up an integrated perspective for understanding the complex biological regulatory mechanisms during the germination process of C. semen.

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Enhancement of γ-aminobutyric acid (GABA) and other health-promoting metabolites in germinated broccoli by mannose treatment

Cited by 12 publications

References 31 publications

Calcium Involved in the Enrichment of γ-Aminobutyric Acid (GABA) in Broccoli Sprouts under Fructose Treatment

Calcium Involved in the Enrichment of γ-Aminobutyric Acid (GABA) in Broccoli Sprouts under Fructose Treatment

· Integrated metabolic and transcriptomic profiles reveal the germination-associated dynamic changes for Cassiae Semen

Integrated metabolic and transcriptomic profiles reveal the germination‐associated dynamic changes for the seeds of Cassia obtusifolia L.

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