Enhancement of thermal uniformity for a microthermal cycler and its application for polymerase chain reaction☆

Hsieh, Tar‐Hwa; Luo, Ching‐Hsing; Huang, Fuchun; Wang, Jung-Hao; Chien, Liang-Ju; Lee, Gwo‐Bin

doi:10.1016/j.snb.2007.10.063

Cited by 59 publications

(52 citation statements)

References 23 publications

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“…At the lower part, a heater and a sensor were attached; the upper PCB part was fabricated to cool or transfer the heat to the chamber through the substrate after attaching a thermal pad; the holes in the cover were divided into inlet/outlet holes and an air hole. However, the micro-PCR chip proposed in this paper consists of a four-layer structure, as shown in Figure 2.At the very bottom, a heating circuit for heating, with the PCB base and a thermal sensor for sensing temperature, are attached [13][14][15].…”

Section: The Proposed Pcr Chip Structurementioning

confidence: 99%

Black Printed Circuit Board-based Micro-Polymerase Chain ReactionChip Structure for Fluorescence Detection Test

Hwang¹,

Kim²,

Kim³

et al. 2015

IJCA

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Section: The Proposed Pcr Chip Structurementioning

confidence: 99%

Black Printed Circuit Board-based Micro-Polymerase Chain ReactionChip Structure for Fluorescence Detection Test

Hwang¹,

Kim²,

Kim³

et al. 2015

IJCA

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“…However, the proposed micro-PCR chip was modified to have a four-layer structure, as shown in Figure 2. The PCB was at the very bottom; the heating circuit, which was used for heating, and the thermal sensor, which senses temperature, were attached to the PCB [13][14][15].…”

Section: Pcr Chip Structurementioning

confidence: 99%

Implementation of PCB-Based PCR Chip Using Double-Sided Tape

Hwang¹,

Kim²,

Kim³

et al. 2015

IJCA

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“…At the very bottom, a heating circuit for heating, with the PCB base and a thermal sensor for sensing temperature, are attached [12,13,14].…”

Section: Black Pcb-based Micro-pcr Chipmentioning

confidence: 99%

Micro-Polymerase Chain Reaction Chip Construction for Fluorescence Detection Test

Hwang¹,

Kim²,

Song³

et al. 2015

Advanced Science and Technology Letters

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Abstract. This paper proposes the optimal structure of a Printed Circuit Boardbased micro-PCR chip constructed on a PCB substrate using commercial adhesive tapes and plastic films. The proposed micro-PCR chip was composed of four layers. In a previous experiment, the chip had been made with a green PCB, yielding an unwanted reflection that prevented accurate analysis. In this study, the solder mask of the PCB substrate was coated black for a higher signal-to-noise ratio, and the area where the reaction chamber was attached was legend-printed with white silk for better reflection of the fluorescence light. The top cover was also colored black to minimize the noise during fluorescence detection. As a result of carrying out PCR, it was found that the fluorescence brightness increased with increasing numbers of cycles.

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Enhancement of thermal uniformity for a microthermal cycler and its application for polymerase chain reaction☆

Cited by 59 publications

References 23 publications

Black Printed Circuit Board-based Micro-Polymerase Chain ReactionChip Structure for Fluorescence Detection Test

Black Printed Circuit Board-based Micro-Polymerase Chain ReactionChip Structure for Fluorescence Detection Test

Implementation of PCB-Based PCR Chip Using Double-Sided Tape

Micro-Polymerase Chain Reaction Chip Construction for Fluorescence Detection Test

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