According to psychometric properties, the most suitable performance measure for evaluating balance in community-dwelling older people was the TB, followed by the TUG.
This study reports an integrated microfluidic chip for polymerase chain reaction (PCR) applications utilizing digital microfluidic chip (DMC) technology. Several crucial procedures including sample transportation, mixing, and DNA amplification were performed on the integrated chip using electro-wetting-on-dielectric (EWOD) effect. An innovative concept of hydrophobic/hydrophilic structure has been successfully demonstrated to integrate the DMC chip with the on-chip PCR device. Sample droplets were generated, transported and mixed by the EWOD-actuation. Then the mixture droplets were transported to a PCR chamber by utilizing the hydrophilic/hydrophobic interface to generate required surface tension gradient. A micro temperature sensor and two micro heaters inside the PCR chamber along with a controller were used to form a micro temperature control module, which could perform precise PCR thermal cycling for DNA amplification. In order to demonstrate the performance of the integrated DMC/PCR chips, a detection gene for Dengue II virus was successfully amplified and detected. The new integrated DMC/PCR chips only required an operation voltage of 12V(RMS) at a frequency of 3 KHz for digital microfluidic actuation and 9V(DC) for thermal cycling. When compared to its large-scale counterparts for DNA amplification, the developed system consumed less sample and reagent and could reduce the detection time. The developed chips successfully demonstrated the feasibility of Lab-On-a-Chip (LOC) by utilizing EWOD-based digital microfluidics.
The present paper reports a fully automated microfluidic system for the DNA amplification process by integrating an electroosmotic pump, an active micromixer and an on-chip temperature control system. In this DNA amplification process, the cell lysis is initially performed in a micro cell lysis reactor. Extracted DNA samples, primers and reagents are then driven electroosmotically into a mixing region where they are mixed by the active micromixer. The homogeneous mixture is then thermally cycled in a micro-PCR (polymerase chain reaction) chamber to perform DNA amplification. Experimental results show that the proposed device can successfully automate the sample pretreatment operation for DNA amplification, thereby delivering significant time and effort savings. The new microfluidic system, which facilitates cell lysis, sample driving/mixing and DNA amplification, could provide a significant contribution to ongoing efforts to miniaturize bio-analysis systems by utilizing a simple fabrication process and cheap materials.
This study employed least square support vector machine regression (LS-SVM-R) and multi-linear regression (MLR) for statistically downscaling monthly general circulation model (GCM) outputs directly to monthly catchment streamflows. The scope of the study was limited to calibration and validation of the downscaling models. The methodology was demonstrated by its application to a streamflow site in the Grampian water supply system in northwestern Victoria, Australia. Probable predictors for the study were selected from the National Center for Environmental Prediction/National Center for Atmospheric Research (NCEP/NCAR) reanalysis data set based on the past literature and hydrology. Probable variables that displayed the best significant correlations, consistently with the streamflows over the entire period of the study and under three 20-year time slices (1950-1969, 1970-1989 and 1990-2010) were selected as potential predictors. To better capture seasonal variations of streamflows, downscaling models were developed for each calendar month. The standardized potential predictors were introduced to the LS-SVM-R and MLR models, starting from the best correlated three and then, others one by one, based on their correlations with the streamflows, until the model performance in validation was maximized. This stepwise model development enabled the identification of the optimum number of potential variables for each month. The model calibration was performed over the period 1950-1989 and validation was done for 1990-2010. LS-SVM-R model parameter optimization was achieved using simplex algorithm and leave-one-out cross-validation. The MLR models were optimized by minimizing the sum of squared errors. In both modelling techniques, validation was performed as an independent simulation. In calibration, LS-SVM-R and MLR models displayed equally good performances with a trend of under-predicting high flows. During validation, LS-SVM-R outperformed MLR, though both techniques over-predicted most of the streamflows. It was concluded that LS-SVM-R is a better technique for statistically downscaling GCM outputs to streamflows than MLR, but still MLR is a potential technique for the same task.
This study presents an integrated microfluidic chip capable of performing DNA/RNA (deoxyribonucleic acid/ribonucleic acid) amplification, electrokinetic sample injection and separation, and on-line optical detection of nucleic acid products in an automatic mode. In the proposed device, DNA/RNA samples are first replicated using a micromachine-based PCR module or reverse transcription PCR (RT-PCR) module and then transported by a pneumatic micropump to a sample reservoir. The samples are subsequently driven electrokinetically into a microchannel, where they are separated electrophoretically and then detected optically by a buried optical fiber. The various modules of the integrated microfluidic chip are fabricated from cheap bio-compatible materials, such as PDMS, polymethylmethacrylate, and soda-lime glass. The functionality of the proposed device is demonstrated through its successful application to the DNA-based bacterial detection of Streptococcus pneumoniae and the RNA-based detection of Dengue-2 virus. It is shown that the low thermal inertia of the PCR/RT-PCR modules reduces the sample and reagent consumption and shortens the reaction time. With less human intervention, the subsequent DNA separation and detection could be performed in an automatic mode. The integrated microfluidic device proposed in this study represents a crucial contribution to the fields of molecular biology, genetic analysis, infectious disease detection, and other biomedical applications.
A surface acoustic wave (SAW) sensor with Pt coated ZnO nanorods as the selective layer has been investigated for hydrogen detection. The SAW sensor was fabricated based on a 128 degrees YX-LiNbO(3) substrate with a operating frequency of 145 MHz. A dual delay line configuration was adopted to eliminate external environmental fluctuations. The Pt coated ZnO nanorods were chosen as a selective layer due to their high surface-to-volume ratio, large penetration depth, and fast charge diffusion rate. The ZnO nanorods were synthesized by an aqueous solution method and coated with the noble metal Pt as a catalyst. Finally, the SAW sensor responses to humidity and hydrogen were tested. Results show that the sensor is not sensitive to humidity; moreover, the frequency shift for a hydrogen concentration variation of 6000 ppm is 26 kHz while operating at room temperature. It can be concluded that the Pt coated ZnO nanorod based SAW hydrogen sensor exhibits fast response, good sensitivity and short-term repeatability. It is worth noting that not only is the sensor sensitive enough to operate at room temperature, but also it can avoid the influence of humidity.
Signaling by the hedgehog (hh)-class gene pathway is essential for embryogenesis in organisms ranging from Drosophila to human. We have isolated a hh homolog (Hrohh) from a lophotrochozoan species, the glossiphoniid leech, Helobdella robusta, and examined its expression by reverse transcription polymerase chain reaction (RT-PCR) and whole-mount in situ hybridization. The peak of Hrohh expression occurs during organogenesis (stages 10-11). No patterned expression was detected within the segmented portion of the germinal plate during the early stages of segmentation. In stage 10-11 embryos, Hro-hh is expressed in body wall, foregut, anterior and posterior midgut, reproductive organs and in a subset of ganglionic neurons. Evidence that Hro-hh regulates gut formation was obtained using the steroidal alkaloid cyclopamine, which specifically blocks HH signaling. Cyclopamine induced malformation of both foregut and anterior midgut in Helobdella embryos, and no morphologically recognizable gonads were seen. In contrast, no gross abnormalities were observed in the posterior midgut. Segmental ectoderm developed normally, as did body wall musculature and some other mesodermal derivatives, but the mesenchymal cells that normally come to fill most of the coelomic cavities failed to develop. Taken with data from Drosophila and vertebrates, our data suggest that the role of hh-class genes in gut formation and/or neural differentiation is ancestral to the bilaterians, whereas their role in segmentation evolved secondarily within the Ecdysozoa.
Dramatic advances in understanding the development of selected "model" organisms, coupled with the realization that genes which regulate development are often conserved between diverse taxa, have renewed interest in comparative development and evolution. Recent molecular phylogenies seem to be converging on a new consensus "tree," according to which higher bilaterians fall into three major groups, Deuterostoma, Ecdysozoa, and Lophotrochozoa. Commonly studied model systems for development fall almost exclusively within the first two of these groups. Glossiphoniid leeches (phylum Annelida) offer certain advantages for descriptive and experimental embryology per se, and can also serve to represent the lophotrochozoan clade. We present an overview of the development of glossiphoniid leeches, highlighting some current research questions and the potential for comparative cellular and molecular studies.Résumé : Les progrès spectaculaires de la recherche sur le développement d'organismes « modèles » sélectionnés et la constatation que les gènes régulateurs du développement sont souvent conservés d'un taxon à un autre ont ranimé l'intérêt pour leur développement et leur évolution. Les phylogénies moléculaires récentes semblent converger vers un « arbre » concensus nouveau dans lequel les organismes bilatéraux supérieurs appartiennent à l'un ou l'autre de trois groupes principaux, les Deuterostoma, les Ecdysozoa et les Lophotrochozoa. Les systèmes modèles de développement étudiés couramment appartiennent presque exclusivement aux deux premiers de ces groupes. Les sangsues glossiphoniides (phylum Annelida) sont des sujets bien appropriés en embryologie descriptive ou expérimentale et elles peuvent également représenter le groupe des Lophotrochozoa. On trouvera ici une vision globale du développement des sangsues glossiphoniides, dans laquelle sont soulignées les questions courantes en recherche et leur potentiel dans des étu-des comparatives cellulaires et moléculaires. Reviews / Synthèses [Traduit par la Rédaction] 232
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