Enhancement of the Sensitivity of a Lateral Flow Immunoassay by Using the Biotin–Streptavidin System

Serebrennikova, Kseniya V.; Samsonova, J.V.; Осипов, А. И.

doi:10.3103/s0027131418030070

Cited by 15 publications

(5 citation statements)

References 5 publications

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“…A PCT assay utilizing a biotin–streptavidin system enhanced transverse flow immunoassay. The amplification of the analysis signal resulted from the accumulation of AuNP clumps [ 101 ]. Another platinum staining method based on a test strip retained the original properties of colloidal gold while leveraging platinum nanoparticles (PTNPs) with good catalytic activity as a signal label, achieving sensitive and quantitative testing [ 102 ].…”

Section: Immune Colloidal Gold Technique For Pct Detectionmentioning

confidence: 99%

Progress in Procalcitonin Detection Based on Immunoassay

Huang,

Zu,

Zhang

et al. 2024

Research

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Procalcitonin (PCT) serves as a crucial biomarker utilized in diverse clinical contexts, including sepsis diagnosis and emergency departments. Its applications extend to identifying pathogens, assessing infection severity, guiding drug administration, and implementing theranostic strategies. However, current clinical deployed methods cannot meet the needs for accurate or real-time quantitative monitoring of PCT. This review aims to introduce these emerging PCT immunoassay technologies, focusing on analyzing their advantages in improving detection performances, such as easy operation and high precision. The fundamental principles and characteristics of state-of-the-art methods are first introduced, including chemiluminescence, immunofluorescence, latex-enhanced turbidity, enzyme-linked immunosorbent, colloidal gold immunochromatography, and radioimmunoassay. Then, improved methods using new materials and new technologies are briefly described, for instance, the combination with responsive nanomaterials, Raman spectroscopy, and digital microfluidics. Finally, the detection performance parameters of these methods and the clinical importance of PCT detection are also discussed.

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Section: Immune Colloidal Gold Technique For Pct Detectionmentioning

confidence: 99%

Progress in Procalcitonin Detection Based on Immunoassay

Huang,

Zu,

Zhang

et al. 2024

Research

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“…The successful functionalization of the nanoparticles with neutravidin was confirmed by testing them against the biotin printed across the membrane as the test line. Streptavidin molecules have four binding sites with an extraordinary affinity for biotin [52,53]. Therefore, when neutravidin is on the nanoparticle surface, the complex formed will become trapped in the biotin line.…”

Section: Biosensing Applicationmentioning

confidence: 99%

Double-Layer Fatty Acid Nanoparticles as a Multiplatform for Diagnostics and Therapy

et al. 2022

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Today, public health is one of the most important challenges in society. Cancer is the leading cause of death, so early diagnosis and localized treatments that minimize side effects are a priority. Magnetic nanoparticles have shown great potential as magnetic resonance imaging contrast agents, detection tags for in vitro biosensing, and mediators of heating in magnetic hyperthermia. One of the critical characteristics of nanoparticles to adjust to the biomedical needs of each application is their polymeric coating. Fatty acid coatings are known to contribute to colloidal stability and good surface crystalline quality. While monolayer coatings make the particles hydrophobic, a fatty acid double-layer renders them hydrophilic, and therefore suitable for use in body fluids. In addition, they provide the particles with functional chemical groups that allow their bioconjugation. This work analyzes three types of self-assembled bilayer fatty acid coatings of superparamagnetic iron oxide nanoparticles: oleic, lauric, and myristic acids. We characterize the particles magnetically and structurally and study their potential for resonance imaging, magnetic hyperthermia, and labeling for biosensing in lateral flow immunoassays. We found that the myristic acid sample reported a large r2 relaxivity, superior to existing iron-based commercial agents. For magnetic hyperthermia, a significant specific absorption rate value was obtained for the oleic sample. Finally, the lauric acid sample showed promising results for nanolabeling.

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“…The combination of the specificity and sensitivity in the detection method could lead to the multi-stage amplification of biological signals (Meyer, Sauerwein, & Mutayoba, 1990;Sai et al, 2010). Streptavidin-biotin system also used to combine with materials like nanoparticles in flow immunoassay (Serebrennikova, Samsonova, & Osipov, 2018), immunomagnetic beads (Ming et al, 2018;Shan et al, 2014) and sandwich immunosensor (Zhang et al, 2018) to improve the specificity and sensitivity of the detection methods.…”

Section: Specificitymentioning

confidence: 99%

High efficient chemiluminescent immunoassays for the detection of diclazuril in chicken muscle based on biotin–streptavidin system

Wang

et al. 2020

Food and Agricultural Immunology

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A simple, sensitive and specific chemiluminescence enzyme immunoassay based on biotin-streptavidin system (BASA-CLEIA) for diclazuril was established, which was about 2 and 4 times more sensitive than the traditional chemiluminescence enzyme immunoassay (CLEIA) and enzyme-linked immunosorbent assay. In the optimized generic assay, the half maximal inhibitory concentration (IC 50) of the BASA-CLEIA for diclazuril was 0.48 μg/kg. The limit of detection for diclazuril was 0.02 μg/kg. This method showed no cross reactivity (<0.01%) with toltrazuril, robenidine hydrochloride, nicarbazin, halofuginone, amprolium, monensin, and maduramycin. In chicken muscles, the average recoveries ranged from 90.3% to 120.4%. A further comparison between the developed BSAS-CLEIA and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for real samples showed good correlation (p < 0.01). The results indicated that the simple, sensitive and specific BASA-CLEIA method could be used for the analysis of diclazuril in real samples.

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Enhancement of the Sensitivity of a Lateral Flow Immunoassay by Using the Biotin–Streptavidin System

Cited by 15 publications

References 5 publications

Progress in Procalcitonin Detection Based on Immunoassay

Progress in Procalcitonin Detection Based on Immunoassay

Double-Layer Fatty Acid Nanoparticles as a Multiplatform for Diagnostics and Therapy

High efficient chemiluminescent immunoassays for the detection of diclazuril in chicken muscle based on biotin–streptavidin system

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