Enhancement of the Gibberella zeae growth inhibitory lipopeptides from a Bacillus subtilis mutant by ion beam implantation

Liu, J.; Liu, M.; Wang, J.; Yao, Jianming; Renrui, Pan; Zhang, Yu

doi:10.1007/s00253-005-1981-7

Cited by 31 publications

(16 citation statements)

References 22 publications

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“…In our work, the survival rate curve showed "saddle curve" characteristics, which accorded with the previous literatures [7,13,14]. This usually attributed to the result of the comprehensive effects of energy deposition, momentum transfer and mass deposition [3,4,9].…”

Section: Discussionsupporting

confidence: 78%

Genetic Modification of MEOR Bacterium Bacillus licheniformis H Strain by Low Energy Ion Beam Irradiation

Zhang¹,

Li²,

Ting-sheng³

2010

TOBIOTJ

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Abstract:The microbial enhanced oil recovery (MEOR) related microorganism, identified as Bacillus licheniformis by physiological and biochemical analyses and 16S rDNA sequence analyses, was irradiated with 15keV N + at the dose of 0~4 10 15 ions/cm 2 . The "saddle model" survival curve showed that the turning point is the dose of 2.5 10 15 ions/cm 2 , with the survival rate of 23.3% and the positive mutation rate of 36.7%. The single clone fermentation experiments show that with the rising of dose treated, the surface tension of the fermentation broth of each group had the tendency to decrease, while the group treated at the dose of 2.5 10 15 ions/cm 2 had the lowest average surface tension. Ultimately five mutants were screened from the colonies and two trains, M314 and M325 with a stable expression after the evaluation of five generations were screened out and ready for next analysis. TLC and FT-IR of fermentation broth components showed that the effective component was cyclic lipopeptide. These research results laid a basis for further study in low energy ion beam induced mutation breeding of petroleum microbes.

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Section: Discussionsupporting

confidence: 78%

Genetic Modification of MEOR Bacterium Bacillus licheniformis H Strain by Low Energy Ion Beam Irradiation

Zhang¹,

Li²,

Ting-sheng³

2010

TOBIOTJ

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“…The spore germination of G. etunicatum was 19 ± 3.98%, which was less than that of the treatment 1. This result indicated that in addition to the unknown volatile materials, soluble factors, for instance lipopeptides (Liu et al 2005), might also affect the spore germination of G. etunicatum. Furthermore, the hypha of germinated spores in the direct interaction also appeared short and abnormal, as compared with the control (data not shown).…”

Section: Results Inmentioning

confidence: 91%

“…However, in addition to evaluating the ability of microbial inoculants to inhibit pathogen growth and improve the plant resistance (Liu et al 2005), it is also important to assess the effect of these inoculants on other soil-borne beneficial fungi, especially the arbuscular mycorrhizal fungi (AMF). It is well known that AMF are obligate biotrophs, which form mycorrhizal symbioses with a large spectrum of plant species by improving their nutrition, growth and disease resistence (Smith and Read 1997).…”

Section: Introductionmentioning

confidence: 99%

Impact of Bacillus subtilis JA, a biocontrol strain of fungal plant pathogens, on arbuscular mycorrhiza formation in Zea mays

Xiao

Chen

et al. 2007

World J Microbiol Biotechnol

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In the present study, the influence of Bacillus subtilis JA on arbuscular mycorrhizal fungi (AMF) was evaluated by either pot culture or in vitro conditions, respectively. Under the pot culture conditions, the inoculation of B. subtilis JA decreased the frequency (% F) of the root colonization by indigenous arbuscular mycorrhizal fungi and the shoot dry weight of maize (Zea mays L.), but had no apparent effect on the intensity (% I) of AM fungal root colonization. The unknown volatile emitted from the B. subtilis JA in vitro significantly inhibited spore germination and the hyphal growth in the dual-compartment experiments. Moreover, the data from the direct interaction between B. subtilis JA and Glomus etunicatum showed that soluble antifungal lipopeptides influenced the development of AMF. Therefore, the application of antifungal Bacillus strains should take the compatibility with the indigenous beneficial fungi into consideration.

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“…Now, N ? implantation has been widely used to bombard microorganisms for obtaining industrial strain [7].…”

Section: Introductionmentioning

confidence: 99%

Mutation-Screening in l-(+)-Lactic Acid Producing Strains by Ion Implantation

Zhaoyang

et al. 2011

Indian J Microbiol

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In this paper, in order to obtain some industrial strains with high yield of L-(?)-lactic acid, the wild type strain Lactobacillus casei CICC6028 was mutated by nitrogen ions implantation. By study, it was found that the high positive mutation rate was obtained when the output power was 10 keV and the dose of N ? implantation was 50 9 2.6 9 10 13 ions/cm 2 . In addition, the initial screening methods were also studied, and it was found that the transparent halos method was unavailable, for some high yield strains of L-(?)-lactic acid were missed. Then a mutant strain which was named as N-2 was isolated, its optimum fermentation temperature was 40°C and the L-(?)-lactic acid yield was 136 g/l compared to the original strain whose optimum fermentation temperature was 34°C and L-(?)-lactic acid production was 98 g/l. Finally, High Performance Liquid Chromatography method was used to analyze the purity of L-(?)-lactic acid that was produced by the mutant N-2, and the result showed the main production of N-2 was L-(?)-lactic acid.

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Enhancement of the Gibberella zeae growth inhibitory lipopeptides from a Bacillus subtilis mutant by ion beam implantation

Cited by 31 publications

References 22 publications

Genetic Modification of MEOR Bacterium Bacillus licheniformis H Strain by Low Energy Ion Beam Irradiation

Genetic Modification of MEOR Bacterium Bacillus licheniformis H Strain by Low Energy Ion Beam Irradiation

Impact of Bacillus subtilis JA, a biocontrol strain of fungal plant pathogens, on arbuscular mycorrhiza formation in Zea mays

Mutation-Screening in l-(+)-Lactic Acid Producing Strains by Ion Implantation

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