Enhancement of Tendon-Bone Integration of Anterior Cruciate Ligament Grafts with Bone Morphogenetic Protein-2 Gene Transfer

Martinek, V.; Latterman, Christian; Ūsas, Arvydas; Abramowitch, Steven D.; Woo, Savio L‐Y.; Fu, Freddie H.; Huard, Johnny

doi:10.2106/00004623-200207000-00005

Cited by 244 publications

(180 citation statements)

References 26 publications

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“…Among them, BMP-2 is one of the strongest members (Riley et al 1996). Recently, gene transfer of BMP-2, 4, 7, and 9 was successfully used to achieve in vivo osteoinduction in athymic mice (Lou et al 1999, Krebsbach et al 2000, Musgrave et al 2000, Okubo et al 2001, Abe et al 2002, Zhu et al 2003, repair of radial, femoral, periodontal and cranial bone defects in mice, rats, and rabbits (Lieberman et al 1999, Baltzer et al 2000, Lee et al 2001, Xu et al 2002, Jin et al 2003, Tsuchida et al 2003, Chang et al 2003, spinal fusion in rats, rabbits, and pigs (Riew et al 1998, Dumont et al 2002, Wang et al 2003, and enhancement of tendon-bone integration (Martinek et al 2002). There has been no report, however, of BMP gene transfer inducing the repair of segmental diaphyseal defects in large animals.…”

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confidence: 99%

Immune response and effect of adenovirus-mediated human BMP-2 gene transfer on the repair of segmental tibial bone defects in goats

Tang

Dai

et al. 2005

Acta Orthopaedica

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(2005) Immune response and effect of adenovirus-mediated human BMP-2 gene transfer on the repair of segmental tibial bone defects in goats, Acta Orthopaedica, 76:5, 637-646, DOI: 10.1080 Background Tissue-engineered bone may be used for filling bone defects. There are, however, no reports on this technique used in large animals.Methods We evaluated the effectiveness of, and immune response in repairing diaphyseal bone defects by gene transfer using bone morphogenetic proteins (BMPs). We used adenovirus-mediated human BMP-2 (Adv-hBMP-2) gene-transduced bone marrow stromal cells (BMSCs) to repair 2.1-cm segmental tibial bone defects in goats (group I, n = 7). An Adv-ßgal-transduced BMSC group (group II, n = 5), a non-transduced BMSC group (group III, n = 5), and an untreated group (group IV, n = 2) were used as controls. Self-secreted extracellular matrix was used as cellular carrier.Results Radiographic and histomorphometric examination demonstrated more callus in the bone defects of group I compared to other groups.Week 24 after implantation, the defect healing rates of groups I, II, III, and IV were 6/7, 1/5, 2/5, and 0/2, respectively. The maximum compressive strength of new tissue in the bone defects of group I was higher than those of groups II and III. Temporary cellular and persistent humoral immune responses against adenovirus were detected after hBMP-2 gene transfer.Interpretation We found that Adv-hBMP-2 genetransduced BMSCs had superior osteoinductivity in the repair of tibial bone defects in goats, but it could cause temporary cellular and persistent humoral immune responses against adenovirus.

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confidence: 99%

Immune response and effect of adenovirus-mediated human BMP-2 gene transfer on the repair of segmental tibial bone defects in goats

Tang

Dai

et al. 2005

Acta Orthopaedica

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“…In reality, the regeneration or de novo formation of diseased or traumatized tissues requires the constitution of multiple cell lineages. Once two or more cell types are manipulated in a tissue regeneration process, an interface is created such as the osteochondral interface [6,25,34,35] or the present fibro-osseous interface [36,37]. In these increasingly complex systems, autologous stem cell delivery and/or controlled release of signaling cues may be necessary for engineering the needed interface that segregates two or more different tissue phenotypes [38].…”

Section: Discussionmentioning

confidence: 99%

Autologous stem cell regeneration in craniosynostosis

Moioli¹,

Clark²,

Sumner³

et al. 2008

Bone

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Craniosynostosis occurs in one of 2500 live human births and may manifest as craniofacial disfiguration, seizure, and blindness. Craniotomy is performed to reshape skull bones and resect synostosed cranial sutures. We demonstrate for the first time that autologous mesenchymal stem cells (MSCs) and controlled-released TGFβ3 reduced surgical trauma to localized osteotomy and minimized osteogenesis in a rat craniosynostosis model. Approximately 0.5mL tibial marrow content was aspirated to isolate mononucleated and adherent cells that were characterized as MSCs. Upon resecting the synostosed suture, autologous MSCs in collagen carriers with microencapsulated TGFβ3 (1ng/mL) generated cranial suture analogs characterized as bone-soft tissue-bone interface by quantitative histomorphometric and μCT analyses. Thus, surgical trauma in craniosynostosis can be minimized by a biologically viable implant. We speculate that proportionally larger amounts of human marrow aspirates participate in the healing of craniosynostosis defects in patients. The engineered soft tissue-bone interface may have implications in the repair of tendons, ligaments, periosteum and periodontal ligament.

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“…2004) (Rodeo SA et al 2004) silk fibers, (Altman et GH al. 2002) ) semitendinosus tendon (Martinek V 2002), fibronectin/fibrinogen fibres. (Ahmed Z et al 2000) In addition to these, historically, three major categories of scaffolding materials have been employed.…”

Section: Scaffolds Requirements For Tendon Tissue Engineeringmentioning

confidence: 99%

Regenerative Medicine for Tendon Regeneration and Repair: The Role of Bioscaffolds and Mechanical Loading

Bassetto¹,

Volpin²,

Vindigni³

2011

Biomaterials Science and Engineering

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Enhancement of Tendon-Bone Integration of Anterior Cruciate Ligament Grafts with Bone Morphogenetic Protein-2 Gene Transfer

Cited by 244 publications

References 26 publications

Immune response and effect of adenovirus-mediated human BMP-2 gene transfer on the repair of segmental tibial bone defects in goats

Immune response and effect of adenovirus-mediated human BMP-2 gene transfer on the repair of segmental tibial bone defects in goats

Autologous stem cell regeneration in craniosynostosis

Regenerative Medicine for Tendon Regeneration and Repair: The Role of Bioscaffolds and Mechanical Loading

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